Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis
Abstract Proteus mirabilis (P. mirabilis) is one of the most important causative pathogens associated with complicated urinary tract infections with a 20% incidence. For epidemiological determinations, several phenotypic and molecular typing methods have been implicated. Sixty P. mirabilis isolated...
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BMC
2025-01-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
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| Online Access: | https://doi.org/10.1186/s12941-024-00770-8 |
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| author | Lamiaa A. Salama Hazem Hamed Saleh Shaymaa H. Abdel-Rhman Rasha Barwa Ramadan Hassan |
| author_facet | Lamiaa A. Salama Hazem Hamed Saleh Shaymaa H. Abdel-Rhman Rasha Barwa Ramadan Hassan |
| author_sort | Lamiaa A. Salama |
| collection | DOAJ |
| description | Abstract Proteus mirabilis (P. mirabilis) is one of the most important causative pathogens associated with complicated urinary tract infections with a 20% incidence. For epidemiological determinations, several phenotypic and molecular typing methods have been implicated. Sixty P. mirabilis isolated undergo antibiotic susceptibility test by standard Kirby Bauer method. They showed high resistance to nitrofurantoin and trimethoprim/sulfamethoxazole that appear mainly in 3rd age group. The 2nd age group comprised most of the resistant isolates to the tested antibiotics. A total of 73.33% of isolates were classified as multi drug resistance (MDR) and 78.3% of isolates were distributed in several antibiotypes with MAR index over 0.2. Twenty-one isolates were strong biofilm-producers and they were significantly related to MDR. Different virulence factors as protease, urease and hemolysin production are detected. Detection of several virulence genes by PCR; zapA and ureC were harbored by all isolates, followed by rsbA (95%), ureA and flaA (93%), hpmA (91.7%) and mrpA (73.3%). Determination of genetic diversity between isolates was performed by different methods (RAPD, ISSR, ERIC, BOX-AIR and REP-PCR) by using several parameters as typeability and discriminatory power indicating that ERIC-PCR was the best method followed by REP-PCR 1R. Rand’s & Wallace coefficients were used for calculating the congruence among typing methods. Conclusions: The results obtained from both conventional and molecular typing methods indicated that molecular methods are superior to conventional methods in the discrimination of isolates. ERIC-PCR and Rep-PCR provide high discrimination ability among P. mirabilis clinical isolates contributing to epidemiological studies. |
| format | Article |
| id | doaj-art-8e2d55298163427395a73a1a614a3f6d |
| institution | Kabale University |
| issn | 1476-0711 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj-art-8e2d55298163427395a73a1a614a3f6d2025-01-19T12:13:26ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112025-01-0124111710.1186/s12941-024-00770-8Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilisLamiaa A. Salama0Hazem Hamed Saleh1Shaymaa H. Abdel-Rhman2Rasha Barwa3Ramadan Hassan4Microbiology and Immunology Department, Faculty of Pharmacy, Horus UniversityUrology and Nephrology Center, Mansoura UniversityMicrobiology and Immunology Department, Faculty of Pharmacy, Mansoura UniversityMicrobiology and Immunology Department, Faculty of Pharmacy, Mansoura UniversityMicrobiology and Immunology Department, Faculty of Pharmacy, Mansoura UniversityAbstract Proteus mirabilis (P. mirabilis) is one of the most important causative pathogens associated with complicated urinary tract infections with a 20% incidence. For epidemiological determinations, several phenotypic and molecular typing methods have been implicated. Sixty P. mirabilis isolated undergo antibiotic susceptibility test by standard Kirby Bauer method. They showed high resistance to nitrofurantoin and trimethoprim/sulfamethoxazole that appear mainly in 3rd age group. The 2nd age group comprised most of the resistant isolates to the tested antibiotics. A total of 73.33% of isolates were classified as multi drug resistance (MDR) and 78.3% of isolates were distributed in several antibiotypes with MAR index over 0.2. Twenty-one isolates were strong biofilm-producers and they were significantly related to MDR. Different virulence factors as protease, urease and hemolysin production are detected. Detection of several virulence genes by PCR; zapA and ureC were harbored by all isolates, followed by rsbA (95%), ureA and flaA (93%), hpmA (91.7%) and mrpA (73.3%). Determination of genetic diversity between isolates was performed by different methods (RAPD, ISSR, ERIC, BOX-AIR and REP-PCR) by using several parameters as typeability and discriminatory power indicating that ERIC-PCR was the best method followed by REP-PCR 1R. Rand’s & Wallace coefficients were used for calculating the congruence among typing methods. Conclusions: The results obtained from both conventional and molecular typing methods indicated that molecular methods are superior to conventional methods in the discrimination of isolates. ERIC-PCR and Rep-PCR provide high discrimination ability among P. mirabilis clinical isolates contributing to epidemiological studies.https://doi.org/10.1186/s12941-024-00770-8P. mirabilisAntimicrobial resistanceVirulencePCR-based molecular typingDiscrimination powerCongruence |
| spellingShingle | Lamiaa A. Salama Hazem Hamed Saleh Shaymaa H. Abdel-Rhman Rasha Barwa Ramadan Hassan Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis Annals of Clinical Microbiology and Antimicrobials P. mirabilis Antimicrobial resistance Virulence PCR-based molecular typing Discrimination power Congruence |
| title | Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis |
| title_full | Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis |
| title_fullStr | Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis |
| title_full_unstemmed | Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis |
| title_short | Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis |
| title_sort | assessment of typing methods virulence genes profile and antimicrobial susceptibility for clinical isolates of proteus mirabilis |
| topic | P. mirabilis Antimicrobial resistance Virulence PCR-based molecular typing Discrimination power Congruence |
| url | https://doi.org/10.1186/s12941-024-00770-8 |
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