Isolation and Identification of Pyrene Mineralizing Mycobacterium spp. from Contaminated and Uncontaminated Sources
Mycobacterium isolates obtained from PAH-contaminated and uncontaminated matrices were evaluated for their ability to degrade three-, four- and five-ring PAHs. PAH enrichment studies were prepared using pyrene and inocula obtained from manufacturing gas plant (MGP) soil, uncontaminated agricultural...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Applied and Environmental Soil Science |
| Online Access: | http://dx.doi.org/10.1155/2011/409643 |
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| author | Christopher W. M. Lease Richard H. Bentham Sharyn E. Gaskin Albert L. Juhasz |
| author_facet | Christopher W. M. Lease Richard H. Bentham Sharyn E. Gaskin Albert L. Juhasz |
| author_sort | Christopher W. M. Lease |
| collection | DOAJ |
| description | Mycobacterium isolates obtained from PAH-contaminated and uncontaminated matrices were evaluated for their ability to degrade three-, four- and five-ring PAHs. PAH enrichment studies were prepared using pyrene and inocula obtained from manufacturing gas plant (MGP) soil, uncontaminated agricultural soil, and faeces from Macropus fuliginosus (Western Grey Kangaroo). Three pyrene-degrading microorganisms isolated from the corresponding enrichment cultures had broad substrate ranges, however, isolates could be differentiated based on surfactant, phenol, hydrocarbon and PAH utilisation. 16S rRNA analysis identified all three isolates as Mycobacterium sp. The Mycobacterium spp. could rapidly degrade phenanthrene and pyrene, however, no strain had the capacity to utilise fluorene or benzo[a]pyrene. When pyrene mineralisation experiments were performed, 70–79% of added 14C was evolved as 14CO2 after 10 days. The present study demonstrates that PAH degrading microorganisms may be isolated from a diverse range of environmental matrices. The present study demonstrates that prior exposure to PAHs was not a prerequisite for PAH catabolic activity for two of these Mycobacterium isolates. |
| format | Article |
| id | doaj-art-8e1494651f4848febe1d68dd29838e1f |
| institution | Kabale University |
| issn | 1687-7667 1687-7675 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applied and Environmental Soil Science |
| spelling | doaj-art-8e1494651f4848febe1d68dd29838e1f2025-02-03T01:00:37ZengWileyApplied and Environmental Soil Science1687-76671687-76752011-01-01201110.1155/2011/409643409643Isolation and Identification of Pyrene Mineralizing Mycobacterium spp. from Contaminated and Uncontaminated SourcesChristopher W. M. Lease0Richard H. Bentham1Sharyn E. Gaskin2Albert L. Juhasz3School of Biological Sciences, Flinders University of South Australia, GPO Box 2100, Adelaide, SA 5001, AustraliaEnvironmental Health, School of the Environment, Flinders University of South Australia, GPO Box 2100, Adelaide, SA 5001, AustraliaEnvironmental Health, School of the Environment, Flinders University of South Australia, GPO Box 2100, Adelaide, SA 5001, AustraliaCentre for Environmental Risk Assessment and Remediation, University of South Australia, SA 5095, AustraliaMycobacterium isolates obtained from PAH-contaminated and uncontaminated matrices were evaluated for their ability to degrade three-, four- and five-ring PAHs. PAH enrichment studies were prepared using pyrene and inocula obtained from manufacturing gas plant (MGP) soil, uncontaminated agricultural soil, and faeces from Macropus fuliginosus (Western Grey Kangaroo). Three pyrene-degrading microorganisms isolated from the corresponding enrichment cultures had broad substrate ranges, however, isolates could be differentiated based on surfactant, phenol, hydrocarbon and PAH utilisation. 16S rRNA analysis identified all three isolates as Mycobacterium sp. The Mycobacterium spp. could rapidly degrade phenanthrene and pyrene, however, no strain had the capacity to utilise fluorene or benzo[a]pyrene. When pyrene mineralisation experiments were performed, 70–79% of added 14C was evolved as 14CO2 after 10 days. The present study demonstrates that PAH degrading microorganisms may be isolated from a diverse range of environmental matrices. The present study demonstrates that prior exposure to PAHs was not a prerequisite for PAH catabolic activity for two of these Mycobacterium isolates.http://dx.doi.org/10.1155/2011/409643 |
| spellingShingle | Christopher W. M. Lease Richard H. Bentham Sharyn E. Gaskin Albert L. Juhasz Isolation and Identification of Pyrene Mineralizing Mycobacterium spp. from Contaminated and Uncontaminated Sources Applied and Environmental Soil Science |
| title | Isolation and Identification of Pyrene Mineralizing Mycobacterium spp. from Contaminated and Uncontaminated Sources |
| title_full | Isolation and Identification of Pyrene Mineralizing Mycobacterium spp. from Contaminated and Uncontaminated Sources |
| title_fullStr | Isolation and Identification of Pyrene Mineralizing Mycobacterium spp. from Contaminated and Uncontaminated Sources |
| title_full_unstemmed | Isolation and Identification of Pyrene Mineralizing Mycobacterium spp. from Contaminated and Uncontaminated Sources |
| title_short | Isolation and Identification of Pyrene Mineralizing Mycobacterium spp. from Contaminated and Uncontaminated Sources |
| title_sort | isolation and identification of pyrene mineralizing mycobacterium spp from contaminated and uncontaminated sources |
| url | http://dx.doi.org/10.1155/2011/409643 |
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