Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR
Introduction. Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genus Plasmodium. Misidentification of human malaria parasites especially P. knowlesi based on microscopic examination is very common. The objectives of this paper were to accurately...
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| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2011/104284 |
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| author | Wan Fen Joveen-Neoh Ka Lung Chong Clemente Michael Vui Ling Wong Tiek Ying Lau |
| author_facet | Wan Fen Joveen-Neoh Ka Lung Chong Clemente Michael Vui Ling Wong Tiek Ying Lau |
| author_sort | Wan Fen Joveen-Neoh |
| collection | DOAJ |
| description | Introduction. Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genus Plasmodium. Misidentification of human malaria parasites especially P. knowlesi based on microscopic examination is very common. The objectives of this paper were to accurately identify the incidence of human malaria parasites in the interior division of Sabah, Malaysian Borneo, based on small subunit ribosomal RNA (ssrRNA) and to determine the misidentification rate in human malaria parasites. Methods. Nested PCR was used to detect the presence of human malaria parasites. A total of 243 blood spot samples from patients who had requested for blood film for malaria parasite (BFMP) analyses were used in this study. Results. Nested PCR findings showed that there was no P. malariae infection while the highest prevalent malaria parasite was P. knowlesi, followed by P. vivax, P. falciparum, and mixed infection. Only 69.5% of the 243 samples giving consistent nested PCR and microscopic results. Conclusion. The preliminary findings from molecular detection of malaria showed that P. knowlesi was the most prevalent Plasmodium species in the interior division of Sabah. The findings from this paper may provide a clearer picture on the actual transmission of different Plasmodium species in this region. |
| format | Article |
| id | doaj-art-8e00b70731e64772a5385607493320e1 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-8e00b70731e64772a5385607493320e12025-02-03T06:44:47ZengWileyJournal of Parasitology Research2090-00232090-00312011-01-01201110.1155/2011/104284104284Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCRWan Fen Joveen-Neoh0Ka Lung Chong1Clemente Michael Vui Ling Wong2Tiek Ying Lau3Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaBiotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaBiotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaBiotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaIntroduction. Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genus Plasmodium. Misidentification of human malaria parasites especially P. knowlesi based on microscopic examination is very common. The objectives of this paper were to accurately identify the incidence of human malaria parasites in the interior division of Sabah, Malaysian Borneo, based on small subunit ribosomal RNA (ssrRNA) and to determine the misidentification rate in human malaria parasites. Methods. Nested PCR was used to detect the presence of human malaria parasites. A total of 243 blood spot samples from patients who had requested for blood film for malaria parasite (BFMP) analyses were used in this study. Results. Nested PCR findings showed that there was no P. malariae infection while the highest prevalent malaria parasite was P. knowlesi, followed by P. vivax, P. falciparum, and mixed infection. Only 69.5% of the 243 samples giving consistent nested PCR and microscopic results. Conclusion. The preliminary findings from molecular detection of malaria showed that P. knowlesi was the most prevalent Plasmodium species in the interior division of Sabah. The findings from this paper may provide a clearer picture on the actual transmission of different Plasmodium species in this region.http://dx.doi.org/10.1155/2011/104284 |
| spellingShingle | Wan Fen Joveen-Neoh Ka Lung Chong Clemente Michael Vui Ling Wong Tiek Ying Lau Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR Journal of Parasitology Research |
| title | Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR |
| title_full | Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR |
| title_fullStr | Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR |
| title_full_unstemmed | Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR |
| title_short | Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR |
| title_sort | incidence of malaria in the interior division of sabah malaysian borneo based on nested pcr |
| url | http://dx.doi.org/10.1155/2011/104284 |
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