Genome-wide characterization and expression analysis of the ADF gene family in response to salt and drought stress in alfalfa (Medicago sativa)
The microfilament cytoskeleton, formed by the process of actin polymerization, serves not only to support the morphology of the cell, but also to regulate a number of cellular activities. Actin-depolymerizing factors (ADFs) represent a significant class of actin-binding proteins that regulate the dy...
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| Format: | Article |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1520267/full |
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| author | Mengmeng Shi Yike Wang Peng Lv Yujie Gong Qi Sha Xinyan Zhao Wen Zhou Lingtao Meng Zegang Han Lingxiao Zhang Lingxiao Zhang Yongwang Sun Yongwang Sun |
| author_facet | Mengmeng Shi Yike Wang Peng Lv Yujie Gong Qi Sha Xinyan Zhao Wen Zhou Lingtao Meng Zegang Han Lingxiao Zhang Lingxiao Zhang Yongwang Sun Yongwang Sun |
| author_sort | Mengmeng Shi |
| collection | DOAJ |
| description | The microfilament cytoskeleton, formed by the process of actin polymerization, serves not only to support the morphology of the cell, but also to regulate a number of cellular activities. Actin-depolymerizing factors (ADFs) represent a significant class of actin-binding proteins that regulate the dynamic alterations in the microfilament framework, thereby playing a pivotal role in plant growth and development. Additionally, they are instrumental in modulating stress responses in plants. The ADF gene family has been explored in various plants, but there was a paucity of knowledge regarding the ADF gene family in alfalfa (Medicago sativa), which is one of the most significant leguminous forage crops globally. In this study, a total of nine ADF genes (designated MsADF1 through MsADF9) were identified in the alfalfa genome and mapped to five different chromosomes. A phylogenetic analysis indicated that the MsADF genes could be classified into four distinct groups, with members within the same group exhibiting comparable gene structures and conserved motifs. The analysis of the Ka/Ks ratios indicated that the MsADF genes underwent purity-based selection during its evolutionary expansion. The promoter region of these genes was found to contain multiple cis-acting elements related to hormone responses, defence, and stress, indicating that they may respond to a variety of developmental and environmental stimuli. Gene expression profiles analyzed by RT-qPCR experiments demonstrated that MsADF genes exhibited distinct expression patterns among different organs. Furthermore, the majority of MsADF genes were induced by salt and drought stress by more than two-fold, with MsADF1, 2/3, 6, and 9 being highly induced, suggesting their critical role in resistance to abiotic stress. These results provide comprehensive information on the MsADF gene family in alfalfa and lay a solid foundation for elucidating their biological function. |
| format | Article |
| id | doaj-art-8de57bca04fb4e01a02ddc89108d8894 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-8de57bca04fb4e01a02ddc89108d88942025-01-30T06:23:02ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15202671520267Genome-wide characterization and expression analysis of the ADF gene family in response to salt and drought stress in alfalfa (Medicago sativa)Mengmeng Shi0Yike Wang1Peng Lv2Yujie Gong3Qi Sha4Xinyan Zhao5Wen Zhou6Lingtao Meng7Zegang Han8Lingxiao Zhang9Lingxiao Zhang10Yongwang Sun11Yongwang Sun12College of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaShandong Binnong Technology Co., Ltd., Binzhou, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaShandong Binnong Technology Co., Ltd., Binzhou, ChinaThe microfilament cytoskeleton, formed by the process of actin polymerization, serves not only to support the morphology of the cell, but also to regulate a number of cellular activities. Actin-depolymerizing factors (ADFs) represent a significant class of actin-binding proteins that regulate the dynamic alterations in the microfilament framework, thereby playing a pivotal role in plant growth and development. Additionally, they are instrumental in modulating stress responses in plants. The ADF gene family has been explored in various plants, but there was a paucity of knowledge regarding the ADF gene family in alfalfa (Medicago sativa), which is one of the most significant leguminous forage crops globally. In this study, a total of nine ADF genes (designated MsADF1 through MsADF9) were identified in the alfalfa genome and mapped to five different chromosomes. A phylogenetic analysis indicated that the MsADF genes could be classified into four distinct groups, with members within the same group exhibiting comparable gene structures and conserved motifs. The analysis of the Ka/Ks ratios indicated that the MsADF genes underwent purity-based selection during its evolutionary expansion. The promoter region of these genes was found to contain multiple cis-acting elements related to hormone responses, defence, and stress, indicating that they may respond to a variety of developmental and environmental stimuli. Gene expression profiles analyzed by RT-qPCR experiments demonstrated that MsADF genes exhibited distinct expression patterns among different organs. Furthermore, the majority of MsADF genes were induced by salt and drought stress by more than two-fold, with MsADF1, 2/3, 6, and 9 being highly induced, suggesting their critical role in resistance to abiotic stress. These results provide comprehensive information on the MsADF gene family in alfalfa and lay a solid foundation for elucidating their biological function.https://www.frontiersin.org/articles/10.3389/fpls.2024.1520267/fullactin-depolymerizing factorgene familyalfalfaabiotic stressexpression profile |
| spellingShingle | Mengmeng Shi Yike Wang Peng Lv Yujie Gong Qi Sha Xinyan Zhao Wen Zhou Lingtao Meng Zegang Han Lingxiao Zhang Lingxiao Zhang Yongwang Sun Yongwang Sun Genome-wide characterization and expression analysis of the ADF gene family in response to salt and drought stress in alfalfa (Medicago sativa) Frontiers in Plant Science actin-depolymerizing factor gene family alfalfa abiotic stress expression profile |
| title | Genome-wide characterization and expression analysis of the ADF gene family in response to salt and drought stress in alfalfa (Medicago sativa) |
| title_full | Genome-wide characterization and expression analysis of the ADF gene family in response to salt and drought stress in alfalfa (Medicago sativa) |
| title_fullStr | Genome-wide characterization and expression analysis of the ADF gene family in response to salt and drought stress in alfalfa (Medicago sativa) |
| title_full_unstemmed | Genome-wide characterization and expression analysis of the ADF gene family in response to salt and drought stress in alfalfa (Medicago sativa) |
| title_short | Genome-wide characterization and expression analysis of the ADF gene family in response to salt and drought stress in alfalfa (Medicago sativa) |
| title_sort | genome wide characterization and expression analysis of the adf gene family in response to salt and drought stress in alfalfa medicago sativa |
| topic | actin-depolymerizing factor gene family alfalfa abiotic stress expression profile |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1520267/full |
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