Mast Cells Density Positive to Tryptase Correlates with Angiogenesis in Pancreatic Ductal Adenocarcinoma Patients Having Undergone Surgery
Background. Literature data suggest that cells such as mast cells (MCs), are involved in angiogenesis. MCs can stimulate angiogenesis by releasing of several proangiogenic cytokines stored in their cytoplasm. In particular MCs can release tryptase, a potent in vivo and in vitro proangiogenic facto...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2014/951957 |
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| author | Michele Ammendola Rosario Sacco Giuseppe Sammarco Giuseppe Donato Valeria Zuccalà Maria Luposella Rosa Patruno Ilaria Marech Severino Montemurro Nicola Zizzo Cosmo Damiano Gadaleta Girolamo Ranieri |
| author_facet | Michele Ammendola Rosario Sacco Giuseppe Sammarco Giuseppe Donato Valeria Zuccalà Maria Luposella Rosa Patruno Ilaria Marech Severino Montemurro Nicola Zizzo Cosmo Damiano Gadaleta Girolamo Ranieri |
| author_sort | Michele Ammendola |
| collection | DOAJ |
| description | Background. Literature data suggest that cells such as mast cells (MCs), are involved in angiogenesis. MCs can stimulate angiogenesis by releasing of several proangiogenic cytokines stored in their cytoplasm. In particular MCs can release tryptase, a potent in vivo and in vitro proangiogenic factor. Nevertheless few data are available concerning the role of MCs positive to tryptase in primary pancreatic cancer angiogenesis. This study analyzed MCs and angiogenesis in primary tumour tissue from patients affected by pancreatic ductal adenocarcinoma (PDAC). Method. A series of 31 PDAC patients with stage T2-3N0-1M0 (by AJCC for Pancreas Cancer Staging 7th Edition) was selected and then underwent surgery. Tumour tissue samples were evaluated by means of immunohistochemistry and image analysis methods in terms of number of MCs positive to tryptase (MCDPT), area occupied by MCs positive to tryptase (MCAPT), microvascular density (MVD), and endothelial area (EA). The above parameters were related to each other and to the main clinicopathological features. Results. A significant correlation between MCDPT, MCAPT, MVD, and EA group was found by Pearson’s t-test analysis (r ranged from 0.69 to 0.81; P value ranged from 0.001 to 0.003). No other significant correlation was found. Conclusion. Our pilot data suggest that MCs positive to tryptase may play a role in PDAC angiogenesis and they could be further evaluated as a novel tumour biomarker and as a target of antiangiogenic therapy. |
| format | Article |
| id | doaj-art-8dd2dd1dc74343308a4c6735e932c06d |
| institution | OA Journals |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-8dd2dd1dc74343308a4c6735e932c06d2025-08-20T02:09:41ZengWileyGastroenterology Research and Practice1687-61211687-630X2014-01-01201410.1155/2014/951957951957Mast Cells Density Positive to Tryptase Correlates with Angiogenesis in Pancreatic Ductal Adenocarcinoma Patients Having Undergone SurgeryMichele Ammendola0Rosario Sacco1Giuseppe Sammarco2Giuseppe Donato3Valeria Zuccalà4Maria Luposella5Rosa Patruno6Ilaria Marech7Severino Montemurro8Nicola Zizzo9Cosmo Damiano Gadaleta10Girolamo Ranieri11Department of Medical and Surgery Sciences, Clinical Surgery Unit, University of Catanzaro “Magna Graecia” Medical School, Viale Europa, Germaneto, 88100 Catanzaro, ItalyDepartment of Medical and Surgery Sciences, Clinical Surgery Unit, University of Catanzaro “Magna Graecia” Medical School, Viale Europa, Germaneto, 88100 Catanzaro, ItalyDepartment of Medical and Surgery Sciences, Clinical Surgery Unit, University of Catanzaro “Magna Graecia” Medical School, Viale Europa, Germaneto, 88100 Catanzaro, ItalyHealth Science Department, Pathology Unit, University of Catanzaro “Magna Graecia” Medical School, 88100 Catanzaro, ItalyHealth Science Department, Pathology Unit, University of Catanzaro “Magna Graecia” Medical School, 88100 Catanzaro, ItalyDepartment of Medical and Surgery Sciences, Cardiovascular Disease Unit, University of Catanzaro “Magna Graecia” Medical School, 88100 Catanzaro, ItalyInterventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Giovanni Paolo II, 70100 Bari, ItalyInterventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Giovanni Paolo II, 70100 Bari, ItalySurgery Unit, National Cancer Research Centre, Giovanni Paolo II, 70100 Bari, ItalyChair of Pathology, “Aldo Moro” University of Bari, 70100 Bari, ItalyInterventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Giovanni Paolo II, 70100 Bari, ItalyInterventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Giovanni Paolo II, 70100 Bari, ItalyBackground. Literature data suggest that cells such as mast cells (MCs), are involved in angiogenesis. MCs can stimulate angiogenesis by releasing of several proangiogenic cytokines stored in their cytoplasm. In particular MCs can release tryptase, a potent in vivo and in vitro proangiogenic factor. Nevertheless few data are available concerning the role of MCs positive to tryptase in primary pancreatic cancer angiogenesis. This study analyzed MCs and angiogenesis in primary tumour tissue from patients affected by pancreatic ductal adenocarcinoma (PDAC). Method. A series of 31 PDAC patients with stage T2-3N0-1M0 (by AJCC for Pancreas Cancer Staging 7th Edition) was selected and then underwent surgery. Tumour tissue samples were evaluated by means of immunohistochemistry and image analysis methods in terms of number of MCs positive to tryptase (MCDPT), area occupied by MCs positive to tryptase (MCAPT), microvascular density (MVD), and endothelial area (EA). The above parameters were related to each other and to the main clinicopathological features. Results. A significant correlation between MCDPT, MCAPT, MVD, and EA group was found by Pearson’s t-test analysis (r ranged from 0.69 to 0.81; P value ranged from 0.001 to 0.003). No other significant correlation was found. Conclusion. Our pilot data suggest that MCs positive to tryptase may play a role in PDAC angiogenesis and they could be further evaluated as a novel tumour biomarker and as a target of antiangiogenic therapy.http://dx.doi.org/10.1155/2014/951957 |
| spellingShingle | Michele Ammendola Rosario Sacco Giuseppe Sammarco Giuseppe Donato Valeria Zuccalà Maria Luposella Rosa Patruno Ilaria Marech Severino Montemurro Nicola Zizzo Cosmo Damiano Gadaleta Girolamo Ranieri Mast Cells Density Positive to Tryptase Correlates with Angiogenesis in Pancreatic Ductal Adenocarcinoma Patients Having Undergone Surgery Gastroenterology Research and Practice |
| title | Mast Cells Density Positive to Tryptase Correlates with Angiogenesis in Pancreatic Ductal Adenocarcinoma Patients Having Undergone Surgery |
| title_full | Mast Cells Density Positive to Tryptase Correlates with Angiogenesis in Pancreatic Ductal Adenocarcinoma Patients Having Undergone Surgery |
| title_fullStr | Mast Cells Density Positive to Tryptase Correlates with Angiogenesis in Pancreatic Ductal Adenocarcinoma Patients Having Undergone Surgery |
| title_full_unstemmed | Mast Cells Density Positive to Tryptase Correlates with Angiogenesis in Pancreatic Ductal Adenocarcinoma Patients Having Undergone Surgery |
| title_short | Mast Cells Density Positive to Tryptase Correlates with Angiogenesis in Pancreatic Ductal Adenocarcinoma Patients Having Undergone Surgery |
| title_sort | mast cells density positive to tryptase correlates with angiogenesis in pancreatic ductal adenocarcinoma patients having undergone surgery |
| url | http://dx.doi.org/10.1155/2014/951957 |
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