Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis
The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. The tests were evaluated with use of 185...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/620580 |
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| author | K. T. Sultankulova O. V. Chervyakova N. S. Kozhabergenov K. A. Shorayeva V. M. Strochkov M. B. Orynbayev N. T. Sandybayev A. R. Sansyzbay A. V. Vasin |
| author_facet | K. T. Sultankulova O. V. Chervyakova N. S. Kozhabergenov K. A. Shorayeva V. M. Strochkov M. B. Orynbayev N. T. Sandybayev A. R. Sansyzbay A. V. Vasin |
| author_sort | K. T. Sultankulova |
| collection | DOAJ |
| description | The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. The tests were evaluated with use of 185 specimens from humans, animals, and birds. IAVchip DNA microarray demonstrates higher diagnostic effectiveness (99.45%) in early influenza A diagnosis as compared to the real-time PCR (98.38%) and RT-PCR (96.22%), thus showing its clear superiority. Diagnostic sensitivity of IAVchip DNA microarray (100%) exceeds the same of RT-PCR (95.95%) and real-time RT-PCR (97.96%) in the range of estimated confidence intervals. IAVchip DNA microarray and real-time RT-PCR displayed equal diagnostic specificity (98.85%), while diagnostic specificity of RT-PCR was 96.40%. IAVchip DNA microarray has an advantage over the other tests for influenza A diagnosis and virus identification as a more rapid method that allows performing simultaneous detection and subtyping of about tens of specimens within one experiment during 8–10 hours. The developed IAVchip DNA microarray is a general test tool that enables identifying simultaneously 16 hemagglutinin (HA) and 9 neuraminidase (NA) subtypes of influenza A virus and also to screen the influenza A viruses from humans, animals, and birds by M and NP genes. |
| format | Article |
| id | doaj-art-8dca779ce8674f73857332d98fda6bd2 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-8dca779ce8674f73857332d98fda6bd22025-02-03T05:50:43ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/620580620580Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A DiagnosisK. T. Sultankulova0O. V. Chervyakova1N. S. Kozhabergenov2K. A. Shorayeva3V. M. Strochkov4M. B. Orynbayev5N. T. Sandybayev6A. R. Sansyzbay7A. V. Vasin8Research Institute for Biological Safety Problems (RIBSP), Science Committee of Ministry of Education and Science of Republic of Kazakhstan, Gvardeiskiy, Kordaiskiy Rayon, Zhambylskaya Oblast 080409, KazakhstanResearch Institute for Biological Safety Problems (RIBSP), Science Committee of Ministry of Education and Science of Republic of Kazakhstan, Gvardeiskiy, Kordaiskiy Rayon, Zhambylskaya Oblast 080409, KazakhstanResearch Institute for Biological Safety Problems (RIBSP), Science Committee of Ministry of Education and Science of Republic of Kazakhstan, Gvardeiskiy, Kordaiskiy Rayon, Zhambylskaya Oblast 080409, KazakhstanResearch Institute for Biological Safety Problems (RIBSP), Science Committee of Ministry of Education and Science of Republic of Kazakhstan, Gvardeiskiy, Kordaiskiy Rayon, Zhambylskaya Oblast 080409, KazakhstanResearch Institute for Biological Safety Problems (RIBSP), Science Committee of Ministry of Education and Science of Republic of Kazakhstan, Gvardeiskiy, Kordaiskiy Rayon, Zhambylskaya Oblast 080409, KazakhstanResearch Institute for Biological Safety Problems (RIBSP), Science Committee of Ministry of Education and Science of Republic of Kazakhstan, Gvardeiskiy, Kordaiskiy Rayon, Zhambylskaya Oblast 080409, KazakhstanResearch Institute for Biological Safety Problems (RIBSP), Science Committee of Ministry of Education and Science of Republic of Kazakhstan, Gvardeiskiy, Kordaiskiy Rayon, Zhambylskaya Oblast 080409, KazakhstanResearch Institute for Biological Safety Problems (RIBSP), Science Committee of Ministry of Education and Science of Republic of Kazakhstan, Gvardeiskiy, Kordaiskiy Rayon, Zhambylskaya Oblast 080409, KazakhstanResearch Institute of Influenza, Ministry of Healthcare of the Russian Federation, Prof. Popov Street 15/17, Saint Petersburg 197376, RussiaThe paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. The tests were evaluated with use of 185 specimens from humans, animals, and birds. IAVchip DNA microarray demonstrates higher diagnostic effectiveness (99.45%) in early influenza A diagnosis as compared to the real-time PCR (98.38%) and RT-PCR (96.22%), thus showing its clear superiority. Diagnostic sensitivity of IAVchip DNA microarray (100%) exceeds the same of RT-PCR (95.95%) and real-time RT-PCR (97.96%) in the range of estimated confidence intervals. IAVchip DNA microarray and real-time RT-PCR displayed equal diagnostic specificity (98.85%), while diagnostic specificity of RT-PCR was 96.40%. IAVchip DNA microarray has an advantage over the other tests for influenza A diagnosis and virus identification as a more rapid method that allows performing simultaneous detection and subtyping of about tens of specimens within one experiment during 8–10 hours. The developed IAVchip DNA microarray is a general test tool that enables identifying simultaneously 16 hemagglutinin (HA) and 9 neuraminidase (NA) subtypes of influenza A virus and also to screen the influenza A viruses from humans, animals, and birds by M and NP genes.http://dx.doi.org/10.1155/2014/620580 |
| spellingShingle | K. T. Sultankulova O. V. Chervyakova N. S. Kozhabergenov K. A. Shorayeva V. M. Strochkov M. B. Orynbayev N. T. Sandybayev A. R. Sansyzbay A. V. Vasin Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis The Scientific World Journal |
| title | Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis |
| title_full | Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis |
| title_fullStr | Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis |
| title_full_unstemmed | Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis |
| title_short | Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis |
| title_sort | comparative evaluation of effectiveness of iavchip dna microarray in influenza a diagnosis |
| url | http://dx.doi.org/10.1155/2014/620580 |
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