Intravenous Administration of Adipose-Derived Stem Cell Protein Extracts Improves Neurological Deficits in a Rat Model of Stroke
Treatment of adipose-derived stem cell (ADSC) substantially improves the neurological deficits during stroke by reducing neuronal injury, limiting proinflammatory immune responses, and promoting neuronal repair, which makes ADSC-based therapy an attractive approach for treating stroke. However, the...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/2153629 |
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| author | Kai Zhao Rui Li Changcong Gu Long Liu Yulong Jia Xize Guo Wanping Zhang Chunying Pei Linlu Tian Bo Li Jianrong Jia Huakun Cheng Hongwei Xu Lixian Li |
| author_facet | Kai Zhao Rui Li Changcong Gu Long Liu Yulong Jia Xize Guo Wanping Zhang Chunying Pei Linlu Tian Bo Li Jianrong Jia Huakun Cheng Hongwei Xu Lixian Li |
| author_sort | Kai Zhao |
| collection | DOAJ |
| description | Treatment of adipose-derived stem cell (ADSC) substantially improves the neurological deficits during stroke by reducing neuronal injury, limiting proinflammatory immune responses, and promoting neuronal repair, which makes ADSC-based therapy an attractive approach for treating stroke. However, the potential risk of tumorigenicity and low survival rate of the implanted cells limit the clinical use of ADSC. Cell-free extracts from ADSC (ADSC-E) may be a feasible approach that could overcome these limitations. Here, we aim to explore the potential usage of ADSC-E in treating rat transient middle cerebral artery occlusion (tMCAO). We demonstrated that intravenous (IV) injection of ADSC-E remarkably reduces the ischemic lesion and number of apoptotic neurons as compared to other control groups. Although ADSC and ADSC-E treatment results in a similar degree of a long-term clinical beneficial outcome, the dynamics between two ADSC-based therapies are different. While the injection of ADSC leads to a relatively mild but prolonged therapeutic effect, the administration of ADSC-E results in a fast and pronounced clinical improvement which was associated with a unique change in the molecular signature suggesting that potential mechanisms underlying different therapeutic approach may be different. Together these data provide translational evidence for using protein extracts from ADSC for treating stroke. |
| format | Article |
| id | doaj-art-8da28bfb6bb94c2f9c712fc1411ef9d2 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-8da28bfb6bb94c2f9c712fc1411ef9d22025-02-03T05:44:28ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/21536292153629Intravenous Administration of Adipose-Derived Stem Cell Protein Extracts Improves Neurological Deficits in a Rat Model of StrokeKai Zhao0Rui Li1Changcong Gu2Long Liu3Yulong Jia4Xize Guo5Wanping Zhang6Chunying Pei7Linlu Tian8Bo Li9Jianrong Jia10Huakun Cheng11Hongwei Xu12Lixian Li13Department of Neurosurgery, The First Affiliate Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, ChinaNeuroinflammation Unit, Montreal Neurological Institute, McGill University, Montreal, QC, H3A 2B4, CanadaDepartment of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, ChinaDepartment of Neurosurgery, The First Affiliate Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, ChinaDepartment of Neurosurgery, The First Affiliate Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, ChinaDepartment of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, ChinaDepartment of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, ChinaDepartment of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, ChinaDepartment of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, ChinaDepartment of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, ChinaDepartment of Neurosurgery, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150001, ChinaDepartment of Neurosurgery, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150001, ChinaDepartment of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, ChinaDepartment of Neurosurgery, The First Affiliate Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, ChinaTreatment of adipose-derived stem cell (ADSC) substantially improves the neurological deficits during stroke by reducing neuronal injury, limiting proinflammatory immune responses, and promoting neuronal repair, which makes ADSC-based therapy an attractive approach for treating stroke. However, the potential risk of tumorigenicity and low survival rate of the implanted cells limit the clinical use of ADSC. Cell-free extracts from ADSC (ADSC-E) may be a feasible approach that could overcome these limitations. Here, we aim to explore the potential usage of ADSC-E in treating rat transient middle cerebral artery occlusion (tMCAO). We demonstrated that intravenous (IV) injection of ADSC-E remarkably reduces the ischemic lesion and number of apoptotic neurons as compared to other control groups. Although ADSC and ADSC-E treatment results in a similar degree of a long-term clinical beneficial outcome, the dynamics between two ADSC-based therapies are different. While the injection of ADSC leads to a relatively mild but prolonged therapeutic effect, the administration of ADSC-E results in a fast and pronounced clinical improvement which was associated with a unique change in the molecular signature suggesting that potential mechanisms underlying different therapeutic approach may be different. Together these data provide translational evidence for using protein extracts from ADSC for treating stroke.http://dx.doi.org/10.1155/2017/2153629 |
| spellingShingle | Kai Zhao Rui Li Changcong Gu Long Liu Yulong Jia Xize Guo Wanping Zhang Chunying Pei Linlu Tian Bo Li Jianrong Jia Huakun Cheng Hongwei Xu Lixian Li Intravenous Administration of Adipose-Derived Stem Cell Protein Extracts Improves Neurological Deficits in a Rat Model of Stroke Stem Cells International |
| title | Intravenous Administration of Adipose-Derived Stem Cell Protein Extracts Improves Neurological Deficits in a Rat Model of Stroke |
| title_full | Intravenous Administration of Adipose-Derived Stem Cell Protein Extracts Improves Neurological Deficits in a Rat Model of Stroke |
| title_fullStr | Intravenous Administration of Adipose-Derived Stem Cell Protein Extracts Improves Neurological Deficits in a Rat Model of Stroke |
| title_full_unstemmed | Intravenous Administration of Adipose-Derived Stem Cell Protein Extracts Improves Neurological Deficits in a Rat Model of Stroke |
| title_short | Intravenous Administration of Adipose-Derived Stem Cell Protein Extracts Improves Neurological Deficits in a Rat Model of Stroke |
| title_sort | intravenous administration of adipose derived stem cell protein extracts improves neurological deficits in a rat model of stroke |
| url | http://dx.doi.org/10.1155/2017/2153629 |
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