Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of Oxaliplatin
Considering that currently published oxaliplatin (OXPt) analytical methods require complex procedures and equipment, the objective of this paper was to present the validation of a simple, rapid, precise, and specific isocratic HPLC method for quantification of OXPt. Such method will be essential dur...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/812701 |
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| author | Breno Noronha Matos Paula Martins de Oliveira Thaiene Avila Reis Taís Gratieri Marcílio Cunha-Filho Guilherme Martins Gelfuso |
| author_facet | Breno Noronha Matos Paula Martins de Oliveira Thaiene Avila Reis Taís Gratieri Marcílio Cunha-Filho Guilherme Martins Gelfuso |
| author_sort | Breno Noronha Matos |
| collection | DOAJ |
| description | Considering that currently published oxaliplatin (OXPt) analytical methods require complex procedures and equipment, the objective of this paper was to present the validation of a simple, rapid, precise, and specific isocratic HPLC method for quantification of OXPt. Such method will be essential during the future development of innovative, topical drug formulations for the treatment of mucosal and skin cancers. Validated method demonstrated OXPt separation without interference from the solvents or polymers with the most relevance for developing of bioadhesive pharmaceutical drug carries (chitosan and poloxamer). Method was linear (r>0.999) over studied OXPt concentration range (0.5–15.0 µg/mL) with acceptable precision and accuracy. Limit of detection (LOD) and limit of quantification (LOQ) were 0.099 µg/mL and 0.331 µg/mL, respectively. |
| format | Article |
| id | doaj-art-8da01ef03c1d416195a8c161d9db2c22 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-8da01ef03c1d416195a8c161d9db2c222025-02-03T07:25:45ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/812701812701Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of OxaliplatinBreno Noronha Matos0Paula Martins de Oliveira1Thaiene Avila Reis2Taís Gratieri3Marcílio Cunha-Filho4Guilherme Martins Gelfuso5Laboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilLaboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilLaboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilLaboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilLaboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilLaboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilConsidering that currently published oxaliplatin (OXPt) analytical methods require complex procedures and equipment, the objective of this paper was to present the validation of a simple, rapid, precise, and specific isocratic HPLC method for quantification of OXPt. Such method will be essential during the future development of innovative, topical drug formulations for the treatment of mucosal and skin cancers. Validated method demonstrated OXPt separation without interference from the solvents or polymers with the most relevance for developing of bioadhesive pharmaceutical drug carries (chitosan and poloxamer). Method was linear (r>0.999) over studied OXPt concentration range (0.5–15.0 µg/mL) with acceptable precision and accuracy. Limit of detection (LOD) and limit of quantification (LOQ) were 0.099 µg/mL and 0.331 µg/mL, respectively.http://dx.doi.org/10.1155/2015/812701 |
| spellingShingle | Breno Noronha Matos Paula Martins de Oliveira Thaiene Avila Reis Taís Gratieri Marcílio Cunha-Filho Guilherme Martins Gelfuso Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of Oxaliplatin Journal of Chemistry |
| title | Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of Oxaliplatin |
| title_full | Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of Oxaliplatin |
| title_fullStr | Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of Oxaliplatin |
| title_full_unstemmed | Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of Oxaliplatin |
| title_short | Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of Oxaliplatin |
| title_sort | development and validation of a simple and selective analytical hplc method for the quantification of oxaliplatin |
| url | http://dx.doi.org/10.1155/2015/812701 |
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