Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR Analysis
Background: Shiga toxin-producing Escherichia coli (STEC) strains are bacteria that cause gastrointestinal illnesses in humans. The strain E. coli O157:H7 diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome in humans. Cattle and small ruminants are the main reservoirs of E. coli O157:H7. W...
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CIRAD
2024-11-01
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| Series: | Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux |
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| Online Access: | https://revues.cirad.fr/index.php/REMVT/article/view/37397 |
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| author | Sedigheh Mokhtari Yahya Tahamtan Mohammad Kargar Keyvan Tadayon Elham Moazamian |
| author_facet | Sedigheh Mokhtari Yahya Tahamtan Mohammad Kargar Keyvan Tadayon Elham Moazamian |
| author_sort | Sedigheh Mokhtari |
| collection | DOAJ |
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Background: Shiga toxin-producing Escherichia coli (STEC) strains are bacteria that cause gastrointestinal illnesses in humans. The strain E. coli O157:H7 diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome in humans. Cattle and small ruminants are the main reservoirs of E. coli O157:H7. When shed in animal faeces, the bacteria can contaminate soil, water and food. Aim: We genotyped bacterial isolates and identified their genetic associations and source of origin in order to reduce the spread of infections and improve disease control. Methods: An Enterobacterial Repetitive Intergenic Consensus (ERIC) polymerase chain reaction (PCR) was used to determine the genetic relationships and potential cross-contamination among isolates. The genetic fingerprint patterns obtained from the isolates were compared to determine their genetic associations. A total of twenty-two E. coli O157:H7 isolates were identified from samples collected in carcasses and rectums of cattle, sheep, goats, and from fruit juice samples. SMAC agar supplemented with specific nutrients was used to selectively and differentially isolate E. coli O157:H7. The isolates were then subjected to ERIC-PCR using ERIC1 and ERIC2 primers. Results: The genetic relationships and potential cross-contamination among isolates were evaluated using the fingerprint patterns obtained. Their similarities ranged from 46% to 100%. Isolates from different hosts and sources exhibited similar patterns, indicating a high prevalence of specific clones in various hosts. The study revealed the genetic diversity of E. coli O157:H7 collected from identical sources. Conclusion: This study highlights the importance of genotyping and identifying the sources of E. coli O157:H7 strains. Understanding the genetic relationships and potential cross-contamination among isolates can help prevent the spread of infections and improve the efficacy of disease control.
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| format | Article |
| id | doaj-art-8d7fd47861834e02a3475224e7c77e96 |
| institution | Kabale University |
| issn | 0035-1865 1951-6711 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | CIRAD |
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| series | Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux |
| spelling | doaj-art-8d7fd47861834e02a3475224e7c77e962025-01-23T13:28:01ZengCIRADRevue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux0035-18651951-67112024-11-017710.19182/remvt.37397Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR AnalysisSedigheh Mokhtari0Yahya Tahamtan1Mohammad Kargar2Keyvan Tadayon3Elham Moazamian4Department of Microbiology Shiraz Branch, Islamic Azad University, Shiraz, IranMicrobiology Department, Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, IranDepartment of Biology, Zand Institute of Higher Education, Shiraz, IranDepartment of Veterinary Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranDepartment of Microbiology Shiraz Branch, Islamic Azad University, Shiraz, Iran Background: Shiga toxin-producing Escherichia coli (STEC) strains are bacteria that cause gastrointestinal illnesses in humans. The strain E. coli O157:H7 diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome in humans. Cattle and small ruminants are the main reservoirs of E. coli O157:H7. When shed in animal faeces, the bacteria can contaminate soil, water and food. Aim: We genotyped bacterial isolates and identified their genetic associations and source of origin in order to reduce the spread of infections and improve disease control. Methods: An Enterobacterial Repetitive Intergenic Consensus (ERIC) polymerase chain reaction (PCR) was used to determine the genetic relationships and potential cross-contamination among isolates. The genetic fingerprint patterns obtained from the isolates were compared to determine their genetic associations. A total of twenty-two E. coli O157:H7 isolates were identified from samples collected in carcasses and rectums of cattle, sheep, goats, and from fruit juice samples. SMAC agar supplemented with specific nutrients was used to selectively and differentially isolate E. coli O157:H7. The isolates were then subjected to ERIC-PCR using ERIC1 and ERIC2 primers. Results: The genetic relationships and potential cross-contamination among isolates were evaluated using the fingerprint patterns obtained. Their similarities ranged from 46% to 100%. Isolates from different hosts and sources exhibited similar patterns, indicating a high prevalence of specific clones in various hosts. The study revealed the genetic diversity of E. coli O157:H7 collected from identical sources. Conclusion: This study highlights the importance of genotyping and identifying the sources of E. coli O157:H7 strains. Understanding the genetic relationships and potential cross-contamination among isolates can help prevent the spread of infections and improve the efficacy of disease control. https://revues.cirad.fr/index.php/REMVT/article/view/37397RuminantsE. coli O157: H7genetic techniquesPCRIran |
| spellingShingle | Sedigheh Mokhtari Yahya Tahamtan Mohammad Kargar Keyvan Tadayon Elham Moazamian Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR Analysis Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux Ruminants E. coli O157: H7 genetic techniques PCR Iran |
| title | Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR Analysis |
| title_full | Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR Analysis |
| title_fullStr | Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR Analysis |
| title_full_unstemmed | Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR Analysis |
| title_short | Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR Analysis |
| title_sort | genotyping em escherichia coli em o157 h7 strains isolated from cattle and small ruminants in fars province iran using enterobacterial repetitive intergenic consensus pcr analysis |
| topic | Ruminants E. coli O157: H7 genetic techniques PCR Iran |
| url | https://revues.cirad.fr/index.php/REMVT/article/view/37397 |
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