In vitro inhibition mechanisms of Trichoderma yunnanense TM10 against Pyricularia oryzae and Rhizoctonia solani causing blast and sheath blight diseases in rice (Oryza sativa L.)
Blast and sheath blight diseases, caused by Pyricularia oryzae and Rhizoctonia solani, respectively, are major threats to rice production worldwide. Efforts should be made to limit the spread of these phytopathogens, preferably through sustainable methods. In this study, we investigated the antagoni...
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Taylor & Francis Group
2024-12-01
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| Online Access: | http://dx.doi.org/10.1080/26895293.2024.2422845 |
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| author | Dedat Prismantoro Thomas Argyarich Jefferson Sulistya Ika Akbari Mia Miranti Muhamad Shakirin Mispan Febri Doni |
| author_facet | Dedat Prismantoro Thomas Argyarich Jefferson Sulistya Ika Akbari Mia Miranti Muhamad Shakirin Mispan Febri Doni |
| author_sort | Dedat Prismantoro |
| collection | DOAJ |
| description | Blast and sheath blight diseases, caused by Pyricularia oryzae and Rhizoctonia solani, respectively, are major threats to rice production worldwide. Efforts should be made to limit the spread of these phytopathogens, preferably through sustainable methods. In this study, we investigated the antagonistic potential of the local isolate Trichoderma yunnanense TM10 against the pathogenic fungi P. oryzae and R. solani. The results demonstrated that T. yunnanense TM10 inhibited the growth of P. oryzae and R. solani through mechanisms such as competition, mycoparasitism, and antibiosis. Dual culture, filtrate culture, and double plate assays showed that T. yunnanense TM10 could inhibit the growth of both P. oryzae and R. solani. Slide culture and scanning electron microscope (SEM) tests confirmed that T. yunnanense TM10 exhibited mycoparasitic activity, including attachment, coiling, penetration, lysis, and destruction of pathogen hyphae. Additionally, T. yunnanense TM10 was capable of producing cell wall-degrading enzymes (CWDEs) such as chitinase and cellulase, as well as volatile organic compounds (VOCs) such as hydrogen cyanide (HCN). The multifaceted abilities of T. yunnanense TM10 for limiting the growth of P. oryzae and R. solani underscore its potential as an effective biocontrol agent for enhancing productivity and sustainability in rice cultivation. |
| format | Article |
| id | doaj-art-8d6ceae45e274ca68ed11a6ab596f8d4 |
| institution | Kabale University |
| issn | 2689-5307 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| spelling | doaj-art-8d6ceae45e274ca68ed11a6ab596f8d42025-01-20T14:38:00ZengTaylor & Francis GroupAll Life2689-53072024-12-01170110.1080/26895293.2024.24228452422845In vitro inhibition mechanisms of Trichoderma yunnanense TM10 against Pyricularia oryzae and Rhizoctonia solani causing blast and sheath blight diseases in rice (Oryza sativa L.)Dedat Prismantoro0Thomas Argyarich Jefferson1Sulistya Ika Akbari2Mia Miranti3Muhamad Shakirin Mispan4Febri Doni5Universitas PadjadjaranUniversitas PadjadjaranUniversitas PadjadjaranUniversitas PadjadjaranUniversiti MalayaUniversitas PadjadjaranBlast and sheath blight diseases, caused by Pyricularia oryzae and Rhizoctonia solani, respectively, are major threats to rice production worldwide. Efforts should be made to limit the spread of these phytopathogens, preferably through sustainable methods. In this study, we investigated the antagonistic potential of the local isolate Trichoderma yunnanense TM10 against the pathogenic fungi P. oryzae and R. solani. The results demonstrated that T. yunnanense TM10 inhibited the growth of P. oryzae and R. solani through mechanisms such as competition, mycoparasitism, and antibiosis. Dual culture, filtrate culture, and double plate assays showed that T. yunnanense TM10 could inhibit the growth of both P. oryzae and R. solani. Slide culture and scanning electron microscope (SEM) tests confirmed that T. yunnanense TM10 exhibited mycoparasitic activity, including attachment, coiling, penetration, lysis, and destruction of pathogen hyphae. Additionally, T. yunnanense TM10 was capable of producing cell wall-degrading enzymes (CWDEs) such as chitinase and cellulase, as well as volatile organic compounds (VOCs) such as hydrogen cyanide (HCN). The multifaceted abilities of T. yunnanense TM10 for limiting the growth of P. oryzae and R. solani underscore its potential as an effective biocontrol agent for enhancing productivity and sustainability in rice cultivation.http://dx.doi.org/10.1080/26895293.2024.2422845biological controlpyricularia oryzaerhizoctonia solanirice diseasessymbiotic lifestyletrichoderma |
| spellingShingle | Dedat Prismantoro Thomas Argyarich Jefferson Sulistya Ika Akbari Mia Miranti Muhamad Shakirin Mispan Febri Doni In vitro inhibition mechanisms of Trichoderma yunnanense TM10 against Pyricularia oryzae and Rhizoctonia solani causing blast and sheath blight diseases in rice (Oryza sativa L.) All Life biological control pyricularia oryzae rhizoctonia solani rice diseases symbiotic lifestyle trichoderma |
| title | In vitro inhibition mechanisms of Trichoderma yunnanense TM10 against Pyricularia oryzae and Rhizoctonia solani causing blast and sheath blight diseases in rice (Oryza sativa L.) |
| title_full | In vitro inhibition mechanisms of Trichoderma yunnanense TM10 against Pyricularia oryzae and Rhizoctonia solani causing blast and sheath blight diseases in rice (Oryza sativa L.) |
| title_fullStr | In vitro inhibition mechanisms of Trichoderma yunnanense TM10 against Pyricularia oryzae and Rhizoctonia solani causing blast and sheath blight diseases in rice (Oryza sativa L.) |
| title_full_unstemmed | In vitro inhibition mechanisms of Trichoderma yunnanense TM10 against Pyricularia oryzae and Rhizoctonia solani causing blast and sheath blight diseases in rice (Oryza sativa L.) |
| title_short | In vitro inhibition mechanisms of Trichoderma yunnanense TM10 against Pyricularia oryzae and Rhizoctonia solani causing blast and sheath blight diseases in rice (Oryza sativa L.) |
| title_sort | in vitro inhibition mechanisms of trichoderma yunnanense tm10 against pyricularia oryzae and rhizoctonia solani causing blast and sheath blight diseases in rice oryza sativa l |
| topic | biological control pyricularia oryzae rhizoctonia solani rice diseases symbiotic lifestyle trichoderma |
| url | http://dx.doi.org/10.1080/26895293.2024.2422845 |
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