Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential?
Background. A new trend in the treatment for alveolar clefts in patients with cleft lip and palate involves the use of bone tissue engineering strategies to reduce or eliminate the morbidity associated with autologous bone grafting. The use of mesenchymal stem cells—autologous cells obtained from ti...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/7951696 |
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| author | Carla C. G. Pinheiro Alessander Leyendecker Junior Daniela Y. S. Tanikawa José Ricardo Muniz Ferreira Reza Jarrahy Daniela F. Bueno |
| author_facet | Carla C. G. Pinheiro Alessander Leyendecker Junior Daniela Y. S. Tanikawa José Ricardo Muniz Ferreira Reza Jarrahy Daniela F. Bueno |
| author_sort | Carla C. G. Pinheiro |
| collection | DOAJ |
| description | Background. A new trend in the treatment for alveolar clefts in patients with cleft lip and palate involves the use of bone tissue engineering strategies to reduce or eliminate the morbidity associated with autologous bone grafting. The use of mesenchymal stem cells—autologous cells obtained from tissues such as bone marrow and fat—combined with various biomaterials has been proposed as a viable option for use in cleft patients. However, invasive procedures are necessary to obtain the mesenchymal stem cells from these two sources. To eliminate donor site morbidity, noninvasive stem cell sources such as the umbilical cord, orbicularis oris muscle, and deciduous dental pulp have been studied for use in alveolar cleft bone tissue engineering. In this study, we evaluate the osteogenic potential of these various stem cell types. Methods. Ten cellular strains obtained from each different source (umbilical cord, orbicularis oris muscle, or deciduous dental pulp) were induced to osteogenic differentiation in vitro, and the bone matrix deposition of each primary culture was quantified. To evaluate whether greater osteogenic potential of the established mesenchymal stem cell strains was associated with an increase in the expression profile of neural crest genes, real-time qPCR was performed on the following genes: SRY-box 9, SRY-box 10, nerve growth factor receptor, transcription factor AP-2 alpha, and paired box 3. Results. The mesenchymal stem cells obtained from deciduous dental pulp and orbicularis oris muscle demonstrated increased osteogenic potential with significantly more extracellular bone matrix deposition when compared to primary cultures obtained from the umbilical cord after twenty-one days in culture (p=0.007 and p=0.005, respectively). The paired box 3 gene was more highly expressed in the MSCs obtained from deciduous dental pulp and orbicularis oris muscle than in those obtained from the umbilical cord. Conclusion. These results suggest that deciduous dental pulp and orbicularis oris muscle stem cells demonstrate superior osteogenic differentiation potential relative to umbilical cord-derived stem cells and that this increased potential is related to their neural crest origins. Based on these observations, and the distinct translational advantage of incorporating stem cells from noninvasive tissue sources into tissue engineering protocols, greater study of these specific cell lines in the setting of alveolar cleft repair is indicated. |
| format | Article |
| id | doaj-art-8d28b698db99412281488dd9b987c6f1 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
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| series | Stem Cells International |
| spelling | doaj-art-8d28b698db99412281488dd9b987c6f12025-02-03T01:01:22ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/79516967951696Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential?Carla C. G. Pinheiro0Alessander Leyendecker Junior1Daniela Y. S. Tanikawa2José Ricardo Muniz Ferreira3Reza Jarrahy4Daniela F. Bueno5Hospital Sírio-Libanês-Instituto de Ensino e Pesquisa, São Paulo, SP 01308-050, BrazilHospital Sírio-Libanês-Instituto de Ensino e Pesquisa, São Paulo, SP 01308-050, BrazilHospital Sírio-Libanês-Instituto de Ensino e Pesquisa, São Paulo, SP 01308-050, BrazilInstituto Militar de Engenharia (IME), Departamento de Ciências de Materiais, Programa de Pós Graduação em Ciências de Materiais, Rio de Janeiro, RJ 22290-270, BrazilDavid Geffen School of Medicine, Division of Plastic and Reconstructive Surgery, University of California Los Angeles (UCLA), Los Angeles, CA, USAHospital Sírio-Libanês-Instituto de Ensino e Pesquisa, São Paulo, SP 01308-050, BrazilBackground. A new trend in the treatment for alveolar clefts in patients with cleft lip and palate involves the use of bone tissue engineering strategies to reduce or eliminate the morbidity associated with autologous bone grafting. The use of mesenchymal stem cells—autologous cells obtained from tissues such as bone marrow and fat—combined with various biomaterials has been proposed as a viable option for use in cleft patients. However, invasive procedures are necessary to obtain the mesenchymal stem cells from these two sources. To eliminate donor site morbidity, noninvasive stem cell sources such as the umbilical cord, orbicularis oris muscle, and deciduous dental pulp have been studied for use in alveolar cleft bone tissue engineering. In this study, we evaluate the osteogenic potential of these various stem cell types. Methods. Ten cellular strains obtained from each different source (umbilical cord, orbicularis oris muscle, or deciduous dental pulp) were induced to osteogenic differentiation in vitro, and the bone matrix deposition of each primary culture was quantified. To evaluate whether greater osteogenic potential of the established mesenchymal stem cell strains was associated with an increase in the expression profile of neural crest genes, real-time qPCR was performed on the following genes: SRY-box 9, SRY-box 10, nerve growth factor receptor, transcription factor AP-2 alpha, and paired box 3. Results. The mesenchymal stem cells obtained from deciduous dental pulp and orbicularis oris muscle demonstrated increased osteogenic potential with significantly more extracellular bone matrix deposition when compared to primary cultures obtained from the umbilical cord after twenty-one days in culture (p=0.007 and p=0.005, respectively). The paired box 3 gene was more highly expressed in the MSCs obtained from deciduous dental pulp and orbicularis oris muscle than in those obtained from the umbilical cord. Conclusion. These results suggest that deciduous dental pulp and orbicularis oris muscle stem cells demonstrate superior osteogenic differentiation potential relative to umbilical cord-derived stem cells and that this increased potential is related to their neural crest origins. Based on these observations, and the distinct translational advantage of incorporating stem cells from noninvasive tissue sources into tissue engineering protocols, greater study of these specific cell lines in the setting of alveolar cleft repair is indicated.http://dx.doi.org/10.1155/2019/7951696 |
| spellingShingle | Carla C. G. Pinheiro Alessander Leyendecker Junior Daniela Y. S. Tanikawa José Ricardo Muniz Ferreira Reza Jarrahy Daniela F. Bueno Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential? Stem Cells International |
| title | Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential? |
| title_full | Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential? |
| title_fullStr | Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential? |
| title_full_unstemmed | Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential? |
| title_short | Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential? |
| title_sort | is there a noninvasive source of mscs isolated with gmp methods with better osteogenic potential |
| url | http://dx.doi.org/10.1155/2019/7951696 |
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