Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretome
Improvements in the treatment of cartilage require insights into the secretory profile of mesenchymal stem cells (MSCs). Apart from their differentiation potential, MSCs secrete a multitude of molecules with therapeutic properties that benefit chondrogenesis and immunomodulation. Previously, we empl...
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| Language: | English |
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Elsevier
2025-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025006140 |
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| author | Yann D. Ladner Ursula Menzel Keith Thompson Angela R. Armiento Martin J. Stoddart |
| author_facet | Yann D. Ladner Ursula Menzel Keith Thompson Angela R. Armiento Martin J. Stoddart |
| author_sort | Yann D. Ladner |
| collection | DOAJ |
| description | Improvements in the treatment of cartilage require insights into the secretory profile of mesenchymal stem cells (MSCs). Apart from their differentiation potential, MSCs secrete a multitude of molecules with therapeutic properties that benefit chondrogenesis and immunomodulation. Previously, we employed a small-panel microarray to demonstrate differences within conditioned medium (CM) of MSCs that were mechanically stimulated within a joint-mimicking bioreactor and their unloaded controls.This study analyzed the proteomic content within CM from 4 week mechanically loaded MSCs with a larger protein microarray. We examined the chondrogenic effect of CM by administration to MSC and chondrocyte pellet cultures, as well as functional changes in T cell proliferation.CM from mechanically loaded samples shows a promising push towards chondrogenic phenotypes within both pellet cultures. Inhibition of T cell proliferation was also observed. This in vitro model could enhance our understanding how mechanical load induces changes in MSC secretome benefitting cartilage healing. |
| format | Article |
| id | doaj-art-8d22c51090b04daa88e25e71d8b0f485 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-8d22c51090b04daa88e25e71d8b0f4852025-02-02T05:29:03ZengElsevierHeliyon2405-84402025-02-01113e42234Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretomeYann D. Ladner0Ursula Menzel1Keith Thompson2Angela R. Armiento3Martin J. Stoddart4AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos Platz, Switzerland; Institute for Biomechanics, ETH Zurich, Lengghalde 5, CH-8008, Zurich, SwitzerlandAO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos Platz, SwitzerlandUCB Pharma, Slough, UKUCB Pharma, Slough, UKAO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos Platz, Switzerland; Department of Orthopedics and Trauma Surgery, Medical Center-Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, 79106, Freiburg, Germany; Corresponding author. AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos Platz, Switzerland.Improvements in the treatment of cartilage require insights into the secretory profile of mesenchymal stem cells (MSCs). Apart from their differentiation potential, MSCs secrete a multitude of molecules with therapeutic properties that benefit chondrogenesis and immunomodulation. Previously, we employed a small-panel microarray to demonstrate differences within conditioned medium (CM) of MSCs that were mechanically stimulated within a joint-mimicking bioreactor and their unloaded controls.This study analyzed the proteomic content within CM from 4 week mechanically loaded MSCs with a larger protein microarray. We examined the chondrogenic effect of CM by administration to MSC and chondrocyte pellet cultures, as well as functional changes in T cell proliferation.CM from mechanically loaded samples shows a promising push towards chondrogenic phenotypes within both pellet cultures. Inhibition of T cell proliferation was also observed. This in vitro model could enhance our understanding how mechanical load induces changes in MSC secretome benefitting cartilage healing.http://www.sciencedirect.com/science/article/pii/S2405844025006140SecretomeBioreactorMechanical loadingTissue engineeringCartilageMesenchymal stem cells |
| spellingShingle | Yann D. Ladner Ursula Menzel Keith Thompson Angela R. Armiento Martin J. Stoddart Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretome Heliyon Secretome Bioreactor Mechanical loading Tissue engineering Cartilage Mesenchymal stem cells |
| title | Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretome |
| title_full | Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretome |
| title_fullStr | Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretome |
| title_full_unstemmed | Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretome |
| title_short | Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretome |
| title_sort | phenotypic alterations in articulating joint cells role of mechanically loaded msc secretome |
| topic | Secretome Bioreactor Mechanical loading Tissue engineering Cartilage Mesenchymal stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025006140 |
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