Taenia asiatica: Mitochondrial signatures based analysis of an emerging public health threat in India
Background: Taenia asiatica is a zoonotic tapeworm, commonly known as Asian Taenia. It is an emerging sister species of T. saginata with pigs as intermediate hosts. The present study aimed at genetic characterization and population structure analysis of T. asiatica metacestodes in slaughtered pigs i...
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Elsevier
2025-02-01
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| Series: | New Microbes and New Infections |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2052297524003469 |
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| author | Aman D. Moudgil Anil K. Nehra Pallavi Moudgil |
| author_facet | Aman D. Moudgil Anil K. Nehra Pallavi Moudgil |
| author_sort | Aman D. Moudgil |
| collection | DOAJ |
| description | Background: Taenia asiatica is a zoonotic tapeworm, commonly known as Asian Taenia. It is an emerging sister species of T. saginata with pigs as intermediate hosts. The present study aimed at genetic characterization and population structure analysis of T. asiatica metacestodes in slaughtered pigs in Haryana, north India. Methods: In total, the vital organs of 253 slaughtered pigs were screened for the presence of T. asiatica metacestodes. The molecular identification and phylogenetics were performed targeting the mitochondrial NADH dehydrogenase subunit 1 (nad1) and cytochrome C oxidase subunit 1 (cox1) genes. The median-joining haplotype network and population structure analyses were performed with the sequences generated herein and GenBank-archived T. asiatica sequences for both mitochondrial signatures. Results: Out of 253 pigs screened, the liver of only one animal showed the presence of T. asiatica metacestodes. The sequences generated herein exhibited 99.60 % and 98.85 % similarity to the GenBank-archived sequences of T. asiatica corresponding to the nad1 and cox1 genes, respectively. Overall, 2 and 6 haplotypes for the overall data set with low nucleotide (0.00399 ± 0.00237 and 0.00095 ± 0.00042) and low haplotype (0.400 ± 0.237 and 0.131 ± 0.054) diversities were recorded for the nad1 and cox1 genes, respectively. The negative values recorded for the neutrality indices exhibited deviations from neutrality and hence, propounded recent population expansion or purifying selection or selective sweep. Conclusions: The findings of the present study are of significant medical importance considering an emerging global public health threat of the neglected tapeworm T. asiatica. |
| format | Article |
| id | doaj-art-8d21f73cf3364ccd80a2490e42bad819 |
| institution | Kabale University |
| issn | 2052-2975 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | New Microbes and New Infections |
| spelling | doaj-art-8d21f73cf3364ccd80a2490e42bad8192025-01-24T04:44:56ZengElsevierNew Microbes and New Infections2052-29752025-02-0163101562Taenia asiatica: Mitochondrial signatures based analysis of an emerging public health threat in IndiaAman D. Moudgil0Anil K. Nehra1Pallavi Moudgil2Department of Veterinary Parasitology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, 125004, IndiaDepartment of Veterinary Parasitology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, 125004, IndiaDepartment of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, 125004, India; Corresponding author. Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana India.Background: Taenia asiatica is a zoonotic tapeworm, commonly known as Asian Taenia. It is an emerging sister species of T. saginata with pigs as intermediate hosts. The present study aimed at genetic characterization and population structure analysis of T. asiatica metacestodes in slaughtered pigs in Haryana, north India. Methods: In total, the vital organs of 253 slaughtered pigs were screened for the presence of T. asiatica metacestodes. The molecular identification and phylogenetics were performed targeting the mitochondrial NADH dehydrogenase subunit 1 (nad1) and cytochrome C oxidase subunit 1 (cox1) genes. The median-joining haplotype network and population structure analyses were performed with the sequences generated herein and GenBank-archived T. asiatica sequences for both mitochondrial signatures. Results: Out of 253 pigs screened, the liver of only one animal showed the presence of T. asiatica metacestodes. The sequences generated herein exhibited 99.60 % and 98.85 % similarity to the GenBank-archived sequences of T. asiatica corresponding to the nad1 and cox1 genes, respectively. Overall, 2 and 6 haplotypes for the overall data set with low nucleotide (0.00399 ± 0.00237 and 0.00095 ± 0.00042) and low haplotype (0.400 ± 0.237 and 0.131 ± 0.054) diversities were recorded for the nad1 and cox1 genes, respectively. The negative values recorded for the neutrality indices exhibited deviations from neutrality and hence, propounded recent population expansion or purifying selection or selective sweep. Conclusions: The findings of the present study are of significant medical importance considering an emerging global public health threat of the neglected tapeworm T. asiatica.http://www.sciencedirect.com/science/article/pii/S2052297524003469Molecular characterizationMitochondrial genesPhylogenyPigsTaenia asiatica |
| spellingShingle | Aman D. Moudgil Anil K. Nehra Pallavi Moudgil Taenia asiatica: Mitochondrial signatures based analysis of an emerging public health threat in India New Microbes and New Infections Molecular characterization Mitochondrial genes Phylogeny Pigs Taenia asiatica |
| title | Taenia asiatica: Mitochondrial signatures based analysis of an emerging public health threat in India |
| title_full | Taenia asiatica: Mitochondrial signatures based analysis of an emerging public health threat in India |
| title_fullStr | Taenia asiatica: Mitochondrial signatures based analysis of an emerging public health threat in India |
| title_full_unstemmed | Taenia asiatica: Mitochondrial signatures based analysis of an emerging public health threat in India |
| title_short | Taenia asiatica: Mitochondrial signatures based analysis of an emerging public health threat in India |
| title_sort | taenia asiatica mitochondrial signatures based analysis of an emerging public health threat in india |
| topic | Molecular characterization Mitochondrial genes Phylogeny Pigs Taenia asiatica |
| url | http://www.sciencedirect.com/science/article/pii/S2052297524003469 |
| work_keys_str_mv | AT amandmoudgil taeniaasiaticamitochondrialsignaturesbasedanalysisofanemergingpublichealththreatinindia AT anilknehra taeniaasiaticamitochondrialsignaturesbasedanalysisofanemergingpublichealththreatinindia AT pallavimoudgil taeniaasiaticamitochondrialsignaturesbasedanalysisofanemergingpublichealththreatinindia |