Genetic Structure of Endangered Species <i>Anagallis foemina</i> Mill. and Abundant Weed <i>Anagalis arvensis</i> L. Occurring in Segetal Habitats in South-Eastern Poland
In Poland, two species of the genus <i>Anagallis</i> can be found in segetal communities: scarlet pimpernel (<i>Anagallis arvensis</i> L.) and blue pimpernel (<i>Anagallis foemina</i> Mill.). <i>A. arvensis</i> usually has brick-red flowers and is a co...
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2024-12-01
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| author | Ewa Kwiecińska-Poppe Sylwia Sowa Joanna Lech Małgorzata Haliniarz Edyta Paczos-Grzęda |
| author_facet | Ewa Kwiecińska-Poppe Sylwia Sowa Joanna Lech Małgorzata Haliniarz Edyta Paczos-Grzęda |
| author_sort | Ewa Kwiecińska-Poppe |
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| description | In Poland, two species of the genus <i>Anagallis</i> can be found in segetal communities: scarlet pimpernel (<i>Anagallis arvensis</i> L.) and blue pimpernel (<i>Anagallis foemina</i> Mill.). <i>A. arvensis</i> usually has brick-red flowers and is a common weed in arable crops. Meanwhile, <i>A. foemina</i>, with blue flowers, is considered a species at risk of extinction in Poland. Flower colour is not a determinant of species affiliation, as there is a form of <i>Anagallis arvensis</i> f. <i>azurea</i> with blue flowers; thus, it is very difficult to specify the species identity of plants with blue flowers based on the negligible differences in morphology. Therefore, for the determination of species affiliation, the presence of two deletions within the intron of the chloroplastic gene <i>trnL</i> in <i>A. arvensis</i> and their absence in <i>A. foemina</i> were confirmed. The genetic similarity and population structure were established based on DNA polymorphism markers identified via the ISSR (inter simple sequence repeat) and SRAP (sequence-related amplified polymorphism) methods. UPGMA (unweighted pair group method with arithmetic mean) analyses revealed that red-flowered (<i>A. arvensis</i>) and blue-flowered (<i>A. foemina</i>) plants were grouped into two separate groups. Within the <i>A. foemina</i> group, two subgroups were distinguished: the first subgroup included genotypes from the Lublin Upland (LU) and Volhynian Polesie (VP), while the second subgroup consisted of genotypes from Western Volhynian Upland (VU). The within-group genetic diversity of <i>A. arvensis</i> was greater than the diversity within the <i>A. foemina</i> subpopulations. Principal coordinate analysis (PCoA) and STRUCTURE were also used to group samples according to species affiliation and collection site. The results obtained confirm that <i>A. foemina</i> populations in the study area are fragmented and isolated, which may lead to a decrease in their adaptability to environmental changes, reduced reproductive rates, and increased mortality. |
| format | Article |
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| institution | Kabale University |
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| publishDate | 2024-12-01 |
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| spelling | doaj-art-8d1c19694eb2481fbb41b5264d2c1ce52025-01-24T13:16:17ZengMDPI AGAgronomy2073-43952024-12-01151310.3390/agronomy15010003Genetic Structure of Endangered Species <i>Anagallis foemina</i> Mill. and Abundant Weed <i>Anagalis arvensis</i> L. Occurring in Segetal Habitats in South-Eastern PolandEwa Kwiecińska-Poppe0Sylwia Sowa1Joanna Lech2Małgorzata Haliniarz3Edyta Paczos-Grzęda4Department of Herbology and Plant Cultivation Techniques, University of Life Sciences in Lublin, Akademicka Str. 13, 20-950 Lublin, PolandInstitute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Akademicka Str. 15, 20-950 Lublin, PolandInstitute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Akademicka Str. 15, 20-950 Lublin, PolandDepartment of Herbology and Plant Cultivation Techniques, University of Life Sciences in Lublin, Akademicka Str. 13, 20-950 Lublin, PolandInstitute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Akademicka Str. 15, 20-950 Lublin, PolandIn Poland, two species of the genus <i>Anagallis</i> can be found in segetal communities: scarlet pimpernel (<i>Anagallis arvensis</i> L.) and blue pimpernel (<i>Anagallis foemina</i> Mill.). <i>A. arvensis</i> usually has brick-red flowers and is a common weed in arable crops. Meanwhile, <i>A. foemina</i>, with blue flowers, is considered a species at risk of extinction in Poland. Flower colour is not a determinant of species affiliation, as there is a form of <i>Anagallis arvensis</i> f. <i>azurea</i> with blue flowers; thus, it is very difficult to specify the species identity of plants with blue flowers based on the negligible differences in morphology. Therefore, for the determination of species affiliation, the presence of two deletions within the intron of the chloroplastic gene <i>trnL</i> in <i>A. arvensis</i> and their absence in <i>A. foemina</i> were confirmed. The genetic similarity and population structure were established based on DNA polymorphism markers identified via the ISSR (inter simple sequence repeat) and SRAP (sequence-related amplified polymorphism) methods. UPGMA (unweighted pair group method with arithmetic mean) analyses revealed that red-flowered (<i>A. arvensis</i>) and blue-flowered (<i>A. foemina</i>) plants were grouped into two separate groups. Within the <i>A. foemina</i> group, two subgroups were distinguished: the first subgroup included genotypes from the Lublin Upland (LU) and Volhynian Polesie (VP), while the second subgroup consisted of genotypes from Western Volhynian Upland (VU). The within-group genetic diversity of <i>A. arvensis</i> was greater than the diversity within the <i>A. foemina</i> subpopulations. Principal coordinate analysis (PCoA) and STRUCTURE were also used to group samples according to species affiliation and collection site. The results obtained confirm that <i>A. foemina</i> populations in the study area are fragmented and isolated, which may lead to a decrease in their adaptability to environmental changes, reduced reproductive rates, and increased mortality.https://www.mdpi.com/2073-4395/15/1/3<i>Anagallis foemina</i><i>Anagalis arvensis</i><i>Primulaceae</i>ISSRSRAP<i>trnL</i> |
| spellingShingle | Ewa Kwiecińska-Poppe Sylwia Sowa Joanna Lech Małgorzata Haliniarz Edyta Paczos-Grzęda Genetic Structure of Endangered Species <i>Anagallis foemina</i> Mill. and Abundant Weed <i>Anagalis arvensis</i> L. Occurring in Segetal Habitats in South-Eastern Poland Agronomy <i>Anagallis foemina</i> <i>Anagalis arvensis</i> <i>Primulaceae</i> ISSR SRAP <i>trnL</i> |
| title | Genetic Structure of Endangered Species <i>Anagallis foemina</i> Mill. and Abundant Weed <i>Anagalis arvensis</i> L. Occurring in Segetal Habitats in South-Eastern Poland |
| title_full | Genetic Structure of Endangered Species <i>Anagallis foemina</i> Mill. and Abundant Weed <i>Anagalis arvensis</i> L. Occurring in Segetal Habitats in South-Eastern Poland |
| title_fullStr | Genetic Structure of Endangered Species <i>Anagallis foemina</i> Mill. and Abundant Weed <i>Anagalis arvensis</i> L. Occurring in Segetal Habitats in South-Eastern Poland |
| title_full_unstemmed | Genetic Structure of Endangered Species <i>Anagallis foemina</i> Mill. and Abundant Weed <i>Anagalis arvensis</i> L. Occurring in Segetal Habitats in South-Eastern Poland |
| title_short | Genetic Structure of Endangered Species <i>Anagallis foemina</i> Mill. and Abundant Weed <i>Anagalis arvensis</i> L. Occurring in Segetal Habitats in South-Eastern Poland |
| title_sort | genetic structure of endangered species i anagallis foemina i mill and abundant weed i anagalis arvensis i l occurring in segetal habitats in south eastern poland |
| topic | <i>Anagallis foemina</i> <i>Anagalis arvensis</i> <i>Primulaceae</i> ISSR SRAP <i>trnL</i> |
| url | https://www.mdpi.com/2073-4395/15/1/3 |
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