HPLC Method Development and Validation for Simultaneous Analysis of Diclofenac Sodium and Rabeprazole Sodium
A stable, simple, rapid, precise, accurate RP-HPLC method for simultaneous analysis of diclofenac sodium and rabeprazole sodium was developed and validated as per ICH guidelines without need of any internal standard. Separation was carried out using C8 column with triethyl amine buffer (pH 5): aceto...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/873187 |
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| author | A. A. Heda D. D. Gadade J. M. Kathiriya P. K. Puranik |
| author_facet | A. A. Heda D. D. Gadade J. M. Kathiriya P. K. Puranik |
| author_sort | A. A. Heda |
| collection | DOAJ |
| description | A stable, simple, rapid, precise, accurate RP-HPLC method for simultaneous analysis of diclofenac sodium and rabeprazole sodium was developed and validated as per ICH guidelines without need of any internal standard. Separation was carried out using C8 column with triethyl amine buffer (pH 5): acetonitrile (50:50 v/v) as mobile phase with flow rate 2 mL min-1. The detection was carried out at 284 nm. The parameters studied were retention time, linearity and range, accuracy, precision, detection limit, quantitation limit and bench top stability. The proposed method can be used for simultaneous estimation of diclofenac sodium and rabeprazole sodium in bulk drugs and pharmaceutical dosage forms. |
| format | Article |
| id | doaj-art-8d11f9459464405b96cfd89b2b99c0ad |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-8d11f9459464405b96cfd89b2b99c0ad2025-02-03T05:44:22ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017S1S386S39010.1155/2010/873187HPLC Method Development and Validation for Simultaneous Analysis of Diclofenac Sodium and Rabeprazole SodiumA. A. Heda0D. D. Gadade1J. M. Kathiriya2P. K. Puranik3Department of Pharmaceutics, Government College of Pharmacy Vedant Road, Osmanpura, Auragabad-431005, IndiaDepartment of Pharmaceutics, Government College of Pharmacy Vedant Road, Osmanpura, Auragabad-431005, IndiaDepartment of Pharmaceutics, Government College of Pharmacy Vedant Road, Osmanpura, Auragabad-431005, IndiaDepartment of Pharmaceutics, Government College of Pharmacy Vedant Road, Osmanpura, Auragabad-431005, IndiaA stable, simple, rapid, precise, accurate RP-HPLC method for simultaneous analysis of diclofenac sodium and rabeprazole sodium was developed and validated as per ICH guidelines without need of any internal standard. Separation was carried out using C8 column with triethyl amine buffer (pH 5): acetonitrile (50:50 v/v) as mobile phase with flow rate 2 mL min-1. The detection was carried out at 284 nm. The parameters studied were retention time, linearity and range, accuracy, precision, detection limit, quantitation limit and bench top stability. The proposed method can be used for simultaneous estimation of diclofenac sodium and rabeprazole sodium in bulk drugs and pharmaceutical dosage forms.http://dx.doi.org/10.1155/2010/873187 |
| spellingShingle | A. A. Heda D. D. Gadade J. M. Kathiriya P. K. Puranik HPLC Method Development and Validation for Simultaneous Analysis of Diclofenac Sodium and Rabeprazole Sodium E-Journal of Chemistry |
| title | HPLC Method Development and Validation for Simultaneous Analysis of Diclofenac Sodium and Rabeprazole Sodium |
| title_full | HPLC Method Development and Validation for Simultaneous Analysis of Diclofenac Sodium and Rabeprazole Sodium |
| title_fullStr | HPLC Method Development and Validation for Simultaneous Analysis of Diclofenac Sodium and Rabeprazole Sodium |
| title_full_unstemmed | HPLC Method Development and Validation for Simultaneous Analysis of Diclofenac Sodium and Rabeprazole Sodium |
| title_short | HPLC Method Development and Validation for Simultaneous Analysis of Diclofenac Sodium and Rabeprazole Sodium |
| title_sort | hplc method development and validation for simultaneous analysis of diclofenac sodium and rabeprazole sodium |
| url | http://dx.doi.org/10.1155/2010/873187 |
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