Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples
Microbiological contamination of process waters is a current issue for pharmaceutical industries. Traditional methods require several days to obtain results; therefore, rapid microbiological methods are widely requested to shorten time-to-result. Milliflex Quantum was developed for the rapid detecti...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/234858 |
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| _version_ | 1832548409930678272 |
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| author | Hervé Meder Anne Baumstummler Renaud Chollet Sophie Barrier Monika Kukuczka Frédéric Olivieri Esther Welterlin Vincent Beguin Sébastien Ribault |
| author_facet | Hervé Meder Anne Baumstummler Renaud Chollet Sophie Barrier Monika Kukuczka Frédéric Olivieri Esther Welterlin Vincent Beguin Sébastien Ribault |
| author_sort | Hervé Meder |
| collection | DOAJ |
| description | Microbiological contamination of process waters is a current issue for pharmaceutical industries. Traditional methods require several days to obtain results; therefore, rapid microbiological methods are widely requested to shorten time-to-result. Milliflex Quantum was developed for the rapid detection and enumeration of microorganisms in filterable samples. It combines membrane filtration to universal fluorescent staining of viable microorganisms. This new alternative method was validated using European and United States Pharmacopeia definitions, with sterile water and/or sterile water artificially contaminated with microorganisms. The Milliflex Quantum method was demonstrated to be reliable, robust, specific, accurate, and linear over the whole range of assays following these guidelines. The Milliflex Quantum system was challenged to detect natural contaminants in different types of pharmaceutical purified process waters. Milliflex Quantum was demonstrated to detect accurately contaminants 3- to 7-fold faster than traditional membrane filtration method. The staining procedure is nondestructive allowing downstream identification following a positive result. The Milliflex Quantum offers a fast, sensitive, and robust alternative to the compendial membrane filtration method. |
| format | Article |
| id | doaj-art-8ce4a568370c40cfa34045de55f341a4 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-8ce4a568370c40cfa34045de55f341a42025-02-03T06:14:08ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/234858234858Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water SamplesHervé Meder0Anne Baumstummler1Renaud Chollet2Sophie Barrier3Monika Kukuczka4Frédéric Olivieri5Esther Welterlin6Vincent Beguin7Sébastien Ribault8Merck Millipore, Lab Solutions, BioMonitoring, Research & Development, Applications Group, 39, Route industrielle de la Hardt, 67120 Molsheim, FranceMerck Millipore, Lab Solutions, BioMonitoring, Research & Development, Applications Group, 39, Route industrielle de la Hardt, 67120 Molsheim, FranceMerck Millipore, Lab Solutions, BioMonitoring, Research & Development, Applications Group, 39, Route industrielle de la Hardt, 67120 Molsheim, FranceMerck Millipore, Lab Solutions, BioMonitoring, Research & Development, Applications Group, 39, Route industrielle de la Hardt, 67120 Molsheim, FranceMerck Millipore, Lab Solutions, BioMonitoring, Research & Development, Applications Group, 39, Route industrielle de la Hardt, 67120 Molsheim, FranceMerck Millipore, Lab Solutions, BioMonitoring, Research & Development, Applications Group, 39, Route industrielle de la Hardt, 67120 Molsheim, FranceMerck Millipore, Lab Solutions, BioMonitoring, Research & Development, Applications Group, 39, Route industrielle de la Hardt, 67120 Molsheim, FranceMerck Millipore, Lab Solutions, BioMonitoring, Research & Development, Applications Group, 39, Route industrielle de la Hardt, 67120 Molsheim, FranceMerck Millipore, Lab Solutions, BioMonitoring, Research & Development, Applications Group, 39, Route industrielle de la Hardt, 67120 Molsheim, FranceMicrobiological contamination of process waters is a current issue for pharmaceutical industries. Traditional methods require several days to obtain results; therefore, rapid microbiological methods are widely requested to shorten time-to-result. Milliflex Quantum was developed for the rapid detection and enumeration of microorganisms in filterable samples. It combines membrane filtration to universal fluorescent staining of viable microorganisms. This new alternative method was validated using European and United States Pharmacopeia definitions, with sterile water and/or sterile water artificially contaminated with microorganisms. The Milliflex Quantum method was demonstrated to be reliable, robust, specific, accurate, and linear over the whole range of assays following these guidelines. The Milliflex Quantum system was challenged to detect natural contaminants in different types of pharmaceutical purified process waters. Milliflex Quantum was demonstrated to detect accurately contaminants 3- to 7-fold faster than traditional membrane filtration method. The staining procedure is nondestructive allowing downstream identification following a positive result. The Milliflex Quantum offers a fast, sensitive, and robust alternative to the compendial membrane filtration method.http://dx.doi.org/10.1100/2012/234858 |
| spellingShingle | Hervé Meder Anne Baumstummler Renaud Chollet Sophie Barrier Monika Kukuczka Frédéric Olivieri Esther Welterlin Vincent Beguin Sébastien Ribault Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples The Scientific World Journal |
| title | Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples |
| title_full | Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples |
| title_fullStr | Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples |
| title_full_unstemmed | Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples |
| title_short | Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples |
| title_sort | fluorescence based rapid detection of microbiological contaminants in water samples |
| url | http://dx.doi.org/10.1100/2012/234858 |
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