Fabrication of pH-Responsive Chitosan/Polyvinylpyrrolidone Hydrogels for Controlled Release of Metronidazole and Antibacterial Properties
This research focused on preparing hydrogels with controlled drug release properties to control gastrointestinal tract bacterial infection. Chitosan (CS) and polyvinylpyrrolidone (PVP) were used as the base polymers, with the CS component crosslinked by glutaraldehyde for hydrogel preparation using...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2023/1205092 |
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| author | Zerihun Feyissa Gemechu Deressa Edossa Tariku Bayisa Bedasa Leta Guta Inki |
| author_facet | Zerihun Feyissa Gemechu Deressa Edossa Tariku Bayisa Bedasa Leta Guta Inki |
| author_sort | Zerihun Feyissa |
| collection | DOAJ |
| description | This research focused on preparing hydrogels with controlled drug release properties to control gastrointestinal tract bacterial infection. Chitosan (CS) and polyvinylpyrrolidone (PVP) were used as the base polymers, with the CS component crosslinked by glutaraldehyde for hydrogel preparation using the solution casting technique. The effect of varying glutaraldehyde content in the hydrogels was characterized by the extent of swelling in simulated physiological fluids of pH 1.2, 6.8, and 7.4; the development of porosity; and gel fraction. Functional groups and covalent and hydrogen bonds formed, thermal stability, phase structure, and morphology were characterized by Fourier-transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, and scanning electron microscopy. The results show that the components in the hydrogels have good compatibility and formed honeycomb-like structures. In vitro studies confirmed that the hydrogels have good biodegradability at pH 7.4. Based on these properties, a CS/PVP hydrogel of the ratio of 60 : 40 crosslinked with 600 μL glutaraldehyde was selected for the in-situ loading of 200 mg of the drug metronidazole (MTZ). The hydrogel was characterized for cumulative drug release in the simulated physiological fluids and drug release kinetics using different models and for its antibacterial activity. The best-fit Korsmeyer–Peppas model suggests that MTZ release followed diffusion and swelling-controlled time-dependent non-Fickian transport related to hydrogel erosion. This hydrogel displays enhanced antimicrobial activity against Staphylococcus aureus, and Escherichia coli showed substantial inhibition zones indicating the produced CS/PVP hydrogels are promising candidates for controlled drug release applications. |
| format | Article |
| id | doaj-art-8cbaebf182af4edca4d6cac80b65058a |
| institution | Kabale University |
| issn | 1687-9430 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-8cbaebf182af4edca4d6cac80b65058a2025-02-03T06:42:37ZengWileyInternational Journal of Polymer Science1687-94302023-01-01202310.1155/2023/1205092Fabrication of pH-Responsive Chitosan/Polyvinylpyrrolidone Hydrogels for Controlled Release of Metronidazole and Antibacterial PropertiesZerihun Feyissa0Gemechu Deressa Edossa1Tariku Bayisa Bedasa2Leta Guta Inki3Department of Applied ChemistryDepartment of Applied ChemistryDepartment of Applied ChemistryDepartment of Applied BiologyThis research focused on preparing hydrogels with controlled drug release properties to control gastrointestinal tract bacterial infection. Chitosan (CS) and polyvinylpyrrolidone (PVP) were used as the base polymers, with the CS component crosslinked by glutaraldehyde for hydrogel preparation using the solution casting technique. The effect of varying glutaraldehyde content in the hydrogels was characterized by the extent of swelling in simulated physiological fluids of pH 1.2, 6.8, and 7.4; the development of porosity; and gel fraction. Functional groups and covalent and hydrogen bonds formed, thermal stability, phase structure, and morphology were characterized by Fourier-transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, and scanning electron microscopy. The results show that the components in the hydrogels have good compatibility and formed honeycomb-like structures. In vitro studies confirmed that the hydrogels have good biodegradability at pH 7.4. Based on these properties, a CS/PVP hydrogel of the ratio of 60 : 40 crosslinked with 600 μL glutaraldehyde was selected for the in-situ loading of 200 mg of the drug metronidazole (MTZ). The hydrogel was characterized for cumulative drug release in the simulated physiological fluids and drug release kinetics using different models and for its antibacterial activity. The best-fit Korsmeyer–Peppas model suggests that MTZ release followed diffusion and swelling-controlled time-dependent non-Fickian transport related to hydrogel erosion. This hydrogel displays enhanced antimicrobial activity against Staphylococcus aureus, and Escherichia coli showed substantial inhibition zones indicating the produced CS/PVP hydrogels are promising candidates for controlled drug release applications.http://dx.doi.org/10.1155/2023/1205092 |
| spellingShingle | Zerihun Feyissa Gemechu Deressa Edossa Tariku Bayisa Bedasa Leta Guta Inki Fabrication of pH-Responsive Chitosan/Polyvinylpyrrolidone Hydrogels for Controlled Release of Metronidazole and Antibacterial Properties International Journal of Polymer Science |
| title | Fabrication of pH-Responsive Chitosan/Polyvinylpyrrolidone Hydrogels for Controlled Release of Metronidazole and Antibacterial Properties |
| title_full | Fabrication of pH-Responsive Chitosan/Polyvinylpyrrolidone Hydrogels for Controlled Release of Metronidazole and Antibacterial Properties |
| title_fullStr | Fabrication of pH-Responsive Chitosan/Polyvinylpyrrolidone Hydrogels for Controlled Release of Metronidazole and Antibacterial Properties |
| title_full_unstemmed | Fabrication of pH-Responsive Chitosan/Polyvinylpyrrolidone Hydrogels for Controlled Release of Metronidazole and Antibacterial Properties |
| title_short | Fabrication of pH-Responsive Chitosan/Polyvinylpyrrolidone Hydrogels for Controlled Release of Metronidazole and Antibacterial Properties |
| title_sort | fabrication of ph responsive chitosan polyvinylpyrrolidone hydrogels for controlled release of metronidazole and antibacterial properties |
| url | http://dx.doi.org/10.1155/2023/1205092 |
| work_keys_str_mv | AT zerihunfeyissa fabricationofphresponsivechitosanpolyvinylpyrrolidonehydrogelsforcontrolledreleaseofmetronidazoleandantibacterialproperties AT gemechuderessaedossa fabricationofphresponsivechitosanpolyvinylpyrrolidonehydrogelsforcontrolledreleaseofmetronidazoleandantibacterialproperties AT tarikubayisabedasa fabricationofphresponsivechitosanpolyvinylpyrrolidonehydrogelsforcontrolledreleaseofmetronidazoleandantibacterialproperties AT letagutainki fabricationofphresponsivechitosanpolyvinylpyrrolidonehydrogelsforcontrolledreleaseofmetronidazoleandantibacterialproperties |