Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin
A conundrum has long lingered over association of cytosol elongation factor Tu (EF-Tu) with bacterial surface. Here we investigated it with Acinetobacter baumannii, an emerging opportunistic pathogen associated with a wide spectrum of infectious diseases. The gene for A. baumannii EF-Tu was sequence...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/128705 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550570862313472 |
|---|---|
| author | Shatha F. Dallo Bailin Zhang James Denno Soonbae Hong Anyu Tsai Williams Haskins Jing Yong Ye Tao Weitao |
| author_facet | Shatha F. Dallo Bailin Zhang James Denno Soonbae Hong Anyu Tsai Williams Haskins Jing Yong Ye Tao Weitao |
| author_sort | Shatha F. Dallo |
| collection | DOAJ |
| description | A conundrum has long lingered over association of cytosol elongation factor Tu (EF-Tu) with bacterial surface. Here we investigated it with Acinetobacter baumannii, an emerging opportunistic pathogen associated with a wide spectrum of infectious diseases. The gene for A. baumannii EF-Tu was sequenced, and recombinant EF-Tu was purified for antibody development. EF-Tu on the bacterial surface and the outer membrane vesicles (OMVs) was revealed by immune electron microscopy, and its presence in the outer membrane (OM) and the OMV subproteomes was verified by Western blotting with the EF-Tu antibodies and confirmed by proteomic analyses. EF-Tu in the OM and the OMV subproteomes bound to fibronectin as detected by Western blot and confirmed by a label-free real-time optical sensor. The sensor that originates from photonic crystal structure in a total-Internal-reflection (PC-TIR) configuration was functionalized with fibronectin for characterizing EF-Tu binding. Altogether, with a novel combination of immunological, proteomical, and biophysical assays, these results suggest association of A. baumannii EF-Tu with the bacterial cell surface, OMVs, and fibronectin. |
| format | Article |
| id | doaj-art-8ca4611da85a4ef0a0967dee8f8963e2 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-8ca4611da85a4ef0a0967dee8f8963e22025-02-03T06:06:23ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/128705128705Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and FibronectinShatha F. Dallo0Bailin Zhang1James Denno2Soonbae Hong3Anyu Tsai4Williams Haskins5Jing Yong Ye6Tao Weitao7Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biomedical Engineering, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at Austin, 1 University Station, Austin, TX 78712, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biomedical Engineering, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USAA conundrum has long lingered over association of cytosol elongation factor Tu (EF-Tu) with bacterial surface. Here we investigated it with Acinetobacter baumannii, an emerging opportunistic pathogen associated with a wide spectrum of infectious diseases. The gene for A. baumannii EF-Tu was sequenced, and recombinant EF-Tu was purified for antibody development. EF-Tu on the bacterial surface and the outer membrane vesicles (OMVs) was revealed by immune electron microscopy, and its presence in the outer membrane (OM) and the OMV subproteomes was verified by Western blotting with the EF-Tu antibodies and confirmed by proteomic analyses. EF-Tu in the OM and the OMV subproteomes bound to fibronectin as detected by Western blot and confirmed by a label-free real-time optical sensor. The sensor that originates from photonic crystal structure in a total-Internal-reflection (PC-TIR) configuration was functionalized with fibronectin for characterizing EF-Tu binding. Altogether, with a novel combination of immunological, proteomical, and biophysical assays, these results suggest association of A. baumannii EF-Tu with the bacterial cell surface, OMVs, and fibronectin.http://dx.doi.org/10.1100/2012/128705 |
| spellingShingle | Shatha F. Dallo Bailin Zhang James Denno Soonbae Hong Anyu Tsai Williams Haskins Jing Yong Ye Tao Weitao Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin The Scientific World Journal |
| title | Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin |
| title_full | Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin |
| title_fullStr | Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin |
| title_full_unstemmed | Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin |
| title_short | Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin |
| title_sort | association of acinetobacter baumannii ef tu with cell surface outer membrane vesicles and fibronectin |
| url | http://dx.doi.org/10.1100/2012/128705 |
| work_keys_str_mv | AT shathafdallo associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin AT bailinzhang associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin AT jamesdenno associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin AT soonbaehong associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin AT anyutsai associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin AT williamshaskins associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin AT jingyongye associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin AT taoweitao associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin |