Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study
Purpose. To introduce additional methods to detect and to quantify single pathogens in the complex biofilm formation on an antibacterial dental material. Materials and Methods. A conventional (ST) and an antibacterial dental composite (B) were manufactured. In vitro: specimens were incubated with a...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Advances in Materials Science and Engineering |
| Online Access: | http://dx.doi.org/10.1155/2016/6367234 |
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| author | Birgit Henrich Ilka Hermann Mara Di Giulio Karl Köhrer René Deenen Sugi Sivalingam Ulrike Peters Thomas Beikler Ralf Janda Stefan Rüttermann |
| author_facet | Birgit Henrich Ilka Hermann Mara Di Giulio Karl Köhrer René Deenen Sugi Sivalingam Ulrike Peters Thomas Beikler Ralf Janda Stefan Rüttermann |
| author_sort | Birgit Henrich |
| collection | DOAJ |
| description | Purpose. To introduce additional methods to detect and to quantify single pathogens in the complex biofilm formation on an antibacterial dental material. Materials and Methods. A conventional (ST) and an antibacterial dental composite (B) were manufactured. In vitro: specimens were incubated with a mixture of early colonizers. Bacterial adhesion was analyzed by TaqMan PCR after 8/24 h. In situ: TaqMan PCR and 16S rRNA Next Generation Sequencing (NGS) were performed. Results. In vitro: after 8 h incubation, B was covered by 58.6% of the bacterial amount that was attached to ST. After 24 h, the amount of attached bacteria to ST remained constant on ST only slightly lower on B. In situ: after 8 h the amount of adhering A. viscosus and S. mitis was prominent on ST and reduced on B. NGS revealed that S. sanguinis, S. parasanguinis, and Gemella sanguinis were the mainly attached species with S. sanguinis dominant on ST and S. parasanguinis and G. sanguinis dominant on B. Conclusions. Initial biofilm formation was altered by B. A shift between actinomycetes and streptococci was observed in situ. TaqMan PCR and 16S rRNA NGS revealed comparable results in situ and demonstrated the usefulness of NGS to characterize complex bacterial communities. |
| format | Article |
| id | doaj-art-8ca3844512124b1fb829dee8296ca610 |
| institution | Kabale University |
| issn | 1687-8434 1687-8442 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Materials Science and Engineering |
| spelling | doaj-art-8ca3844512124b1fb829dee8296ca6102025-02-03T01:26:11ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422016-01-01201610.1155/2016/63672346367234Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot StudyBirgit Henrich0Ilka Hermann1Mara Di Giulio2Karl Köhrer3René Deenen4Sugi Sivalingam5Ulrike Peters6Thomas Beikler7Ralf Janda8Stefan Rüttermann9Institute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Heinrich-Heine-University, Universitätsstrasse 1, 40225 Düsseldorf, GermanyInstitute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Heinrich-Heine-University, Universitätsstrasse 1, 40225 Düsseldorf, GermanyLaboratory Bacteriology, Department of Pharmacy, University of “G. D’Annunzio”, Via dei Vestini 31, 66100 Chieti, ItalyBiological and Medical Research Centre (BMFZ), Heinrich-Heine-University, Universitätsstrasse 1, 40225 Düsseldorf, GermanyBiological and Medical Research Centre (BMFZ), Heinrich-Heine-University, Universitätsstrasse 1, 40225 Düsseldorf, GermanyBiological and Medical Research Centre (BMFZ), Heinrich-Heine-University, Universitätsstrasse 1, 40225 Düsseldorf, GermanyCentre of Dentistry, Section of Periodontics, Heinrich-Heine-University, Moorenstrasse 5, 40225 Düsseldorf, GermanyCentre of Dentistry, Section of Periodontics, Heinrich-Heine-University, Moorenstrasse 5, 40225 Düsseldorf, GermanyCentre of Dentistry, Heinrich-Heine-University, Moorenstrasse 5, 40225 Düsseldorf, GermanyCenter for Dentistry and Oral Medicine (Carolinum), Department of Operative Dentistry, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60596 Frankfurt am Main, GermanyPurpose. To introduce additional methods to detect and to quantify single pathogens in the complex biofilm formation on an antibacterial dental material. Materials and Methods. A conventional (ST) and an antibacterial dental composite (B) were manufactured. In vitro: specimens were incubated with a mixture of early colonizers. Bacterial adhesion was analyzed by TaqMan PCR after 8/24 h. In situ: TaqMan PCR and 16S rRNA Next Generation Sequencing (NGS) were performed. Results. In vitro: after 8 h incubation, B was covered by 58.6% of the bacterial amount that was attached to ST. After 24 h, the amount of attached bacteria to ST remained constant on ST only slightly lower on B. In situ: after 8 h the amount of adhering A. viscosus and S. mitis was prominent on ST and reduced on B. NGS revealed that S. sanguinis, S. parasanguinis, and Gemella sanguinis were the mainly attached species with S. sanguinis dominant on ST and S. parasanguinis and G. sanguinis dominant on B. Conclusions. Initial biofilm formation was altered by B. A shift between actinomycetes and streptococci was observed in situ. TaqMan PCR and 16S rRNA NGS revealed comparable results in situ and demonstrated the usefulness of NGS to characterize complex bacterial communities.http://dx.doi.org/10.1155/2016/6367234 |
| spellingShingle | Birgit Henrich Ilka Hermann Mara Di Giulio Karl Köhrer René Deenen Sugi Sivalingam Ulrike Peters Thomas Beikler Ralf Janda Stefan Rüttermann Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study Advances in Materials Science and Engineering |
| title | Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study |
| title_full | Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study |
| title_fullStr | Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study |
| title_full_unstemmed | Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study |
| title_short | Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study |
| title_sort | reexamination in vitro and in situ of an antibacterially modified experimental dental resin composite with molecular methods a pilot study |
| url | http://dx.doi.org/10.1155/2016/6367234 |
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