Specific detection of pigeon parvovirus with TaqMan real-time PCR technology
The viruses of the Parvoviridae family can infect both vertebrate and invertebrate animals. Recently, pigeon parvovirus (PiPV) was detected in the feces of wild urban pigeons. Owing to no specific detection platform for PiPV, studies on the epidemiology of PiPV are still a research gap. To achieve t...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011192 |
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| author | Cuiteng Chen Chunhua Zhu Shuyu Chen Zhen Chen Huanru Fu YuYi Chen Mengyan Zhang Wenyu Zhang Yu Huang Longfei Cheng Chunhe Wan |
| author_facet | Cuiteng Chen Chunhua Zhu Shuyu Chen Zhen Chen Huanru Fu YuYi Chen Mengyan Zhang Wenyu Zhang Yu Huang Longfei Cheng Chunhe Wan |
| author_sort | Cuiteng Chen |
| collection | DOAJ |
| description | The viruses of the Parvoviridae family can infect both vertebrate and invertebrate animals. Recently, pigeon parvovirus (PiPV) was detected in the feces of wild urban pigeons. Owing to no specific detection platform for PiPV, studies on the epidemiology of PiPV are still a research gap. To achieve this goal, in this study, a TaqMan-based fluorescence quantitative PCR (TaqMan‒PCR) technique was established. The specific primers and probes used were designed on the basis of the NS gene characterization of PiPV downloaded from GenBank. After optimization, the established TaqMan‒PCR assay provides a sensitive, accurate, reliable and cost-effective platform for PiPV detection. We found that both YDPS and healthy birds can be found to have PiPV infection through field sample investigations, and we also investigated the presence of PiPV in Fujian, mainland China. Owing to the failure to propagate PiPV in embryos and cells, knowledge of PiPV replication mechanisms in birds still needs further study. In conclusion, a TaqMan-based fluorescence quantitative PCR method was developed, with the advantages of sensitivity, specificity, and reproducibility. This method can be used for further epidemiological monitoring of PiPV infection. |
| format | Article |
| id | doaj-art-8ca197aa032c48478e452e1484fd5a48 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-8ca197aa032c48478e452e1484fd5a482025-01-22T05:40:24ZengElsevierPoultry Science0032-57912025-01-011041104541Specific detection of pigeon parvovirus with TaqMan real-time PCR technologyCuiteng Chen0Chunhua Zhu1Shuyu Chen2Zhen Chen3Huanru Fu4YuYi Chen5Mengyan Zhang6Wenyu Zhang7Yu Huang8Longfei Cheng9Chunhe Wan10Institute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Disease Control and Prevention, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; Corresponding author at: Xi-feng Road No.104, Jiantian village, Jin'an district, Fuzhou city, 350013, China.The viruses of the Parvoviridae family can infect both vertebrate and invertebrate animals. Recently, pigeon parvovirus (PiPV) was detected in the feces of wild urban pigeons. Owing to no specific detection platform for PiPV, studies on the epidemiology of PiPV are still a research gap. To achieve this goal, in this study, a TaqMan-based fluorescence quantitative PCR (TaqMan‒PCR) technique was established. The specific primers and probes used were designed on the basis of the NS gene characterization of PiPV downloaded from GenBank. After optimization, the established TaqMan‒PCR assay provides a sensitive, accurate, reliable and cost-effective platform for PiPV detection. We found that both YDPS and healthy birds can be found to have PiPV infection through field sample investigations, and we also investigated the presence of PiPV in Fujian, mainland China. Owing to the failure to propagate PiPV in embryos and cells, knowledge of PiPV replication mechanisms in birds still needs further study. In conclusion, a TaqMan-based fluorescence quantitative PCR method was developed, with the advantages of sensitivity, specificity, and reproducibility. This method can be used for further epidemiological monitoring of PiPV infection.http://www.sciencedirect.com/science/article/pii/S0032579124011192Pigeon parvovirus (PiPV)NSTaqMan-PCREpidemiological investigation |
| spellingShingle | Cuiteng Chen Chunhua Zhu Shuyu Chen Zhen Chen Huanru Fu YuYi Chen Mengyan Zhang Wenyu Zhang Yu Huang Longfei Cheng Chunhe Wan Specific detection of pigeon parvovirus with TaqMan real-time PCR technology Poultry Science Pigeon parvovirus (PiPV) NS TaqMan-PCR Epidemiological investigation |
| title | Specific detection of pigeon parvovirus with TaqMan real-time PCR technology |
| title_full | Specific detection of pigeon parvovirus with TaqMan real-time PCR technology |
| title_fullStr | Specific detection of pigeon parvovirus with TaqMan real-time PCR technology |
| title_full_unstemmed | Specific detection of pigeon parvovirus with TaqMan real-time PCR technology |
| title_short | Specific detection of pigeon parvovirus with TaqMan real-time PCR technology |
| title_sort | specific detection of pigeon parvovirus with taqman real time pcr technology |
| topic | Pigeon parvovirus (PiPV) NS TaqMan-PCR Epidemiological investigation |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011192 |
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