The complete chloroplast genome and phylogenetic analysis of Cyananthus macrocalyx Franch. 1887 Campanulaceae

Cyananthus macrocalyx Franch. belongs to the genus Cyananthus in the family Campanulaceae. In this study, we sequenced and analyzed its chloroplast genome then constructed a phylogenetic tree to determine its phylogenetic position. The results show: the total length of the chloroplast genome of C. m...

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Bibliographic Details
Main Authors: Hai-Tao Ma, Dan Lei, Qi-Yin Chen, Bei Jiang, Yong-Zeng Zhang
Format: Article
Language:English
Published: Taylor & Francis Group 2025-06-01
Series:Mitochondrial DNA. Part B. Resources
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Online Access:https://www.tandfonline.com/doi/10.1080/23802359.2025.2503403
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Summary:Cyananthus macrocalyx Franch. belongs to the genus Cyananthus in the family Campanulaceae. In this study, we sequenced and analyzed its chloroplast genome then constructed a phylogenetic tree to determine its phylogenetic position. The results show: the total length of the chloroplast genome of C. macrocalyx was 167,964 bp, with a GC content of 37.95%, and the chloroplast genome exhibited a standard quadripartite structure, consisting of a large single-copy (LSC) region (82,884 bp), a small single-copy (SSC) region (8118 bp), and a pair of inverted repeats (IRs) regions (76,962 bp). A total of 112 genes were annotated from C. macrocalyx, including 78 protein-coding genes, 30 transfer RNA (tRNA) genes, and four ribosomal RNA (rRNA) genes. Additionally, 54 simple sequence repeats (SSRs) were detected, most of which were A/T monomeric sequences. Phylogenetic analyses showed that C. macrocalyx is clustered with C. flavus, suggesting a close relationship between the two species. This study provides a valuable resource for the genetics and evolution study of the Cyananthus.
ISSN:2380-2359