Transcriptional profile features in patients with early and late preeclampsia
Aim: to assess the molecular mechanisms in developing various clinical phenotypes of preeclampsia (PE) by analyzing specific placental tissue transcriptome patterns. Materials and Methods. The prospective observational comparative study in parallel groups enrolled 43 pregnant women divided into 2...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
IRBIS LLC
2024-05-01
|
| Series: | Акушерство, гинекология и репродукция |
| Subjects: | |
| Online Access: | https://www.gynecology.su/jour/article/view/1966 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849395164070543360 |
|---|---|
| author | V. E.A. Kotelnikova D. E. Pantyukhova F. D. Ablyamitova S. N. Vikinskaya Kh. U. Khalilova L. F. Mustafaeva D. A. Barieva D. V. Yarovaya N. D. Chopik M. S. Ermakova L. E. Sorokina |
| author_facet | V. E.A. Kotelnikova D. E. Pantyukhova F. D. Ablyamitova S. N. Vikinskaya Kh. U. Khalilova L. F. Mustafaeva D. A. Barieva D. V. Yarovaya N. D. Chopik M. S. Ermakova L. E. Sorokina |
| author_sort | V. E.A. Kotelnikova |
| collection | DOAJ |
| description | Aim: to assess the molecular mechanisms in developing various clinical phenotypes of preeclampsia (PE) by analyzing specific placental tissue transcriptome patterns. Materials and Methods. The prospective observational comparative study in parallel groups enrolled 43 pregnant women divided into 2 groups: main group – 23 pregnant women with diagnosed PE and control group – 20 apparently healthy women with uncomplicated pregnancy course, delivery and the postpartum period. To examine PE phenotypic features, the main group of pregnant women with PE was subsequently divided into 2 subgroups according to the date of pathology onset: early (n = 10) and late (n = 13) PE. Using the whole-genome next-generation sequencing (NGS), a comparative analysis of altered 18 microRNA level in placental tissue was carried out. Results. Pregnant women with early PE compared to the control group were characterized by significantly low expression level for hsa-miR-656-3p (p < 0.001), hsa-miR-323a-5p (p = 0.017), hsa-miR-519c-3p (p = 0.019), hsa-let-7i-5p (p = 0.019), hsa-miR-433-3p (p = 0.019), hsa-let-7g-5p (p = 0.030), hsa-miR-214-5p (p = 0.030), hsa-miR-27a-5p (p = 0.031), hsa-miR-339-5p (p = 0.041), hsa-miR-524-5p (p = 0.045), hsa-miR-1283 (p = 0.049) and high expression for hsa-miR-151a-5p (p = 0.007), hsa-miR-4521 (p = 0.018), hsa-miR-30d-5p (p = 0.026), hsa-miR-548l (p = 0.027), hsa-miR-133b (p = 0.034), hsa-miR-424-5p (p = 0.042), hsa-miR-211-5p (p = 0.049). Patients with late PE had significantly decreased expression for hsa-miR-656-3p (p = 0.050) andhsa-miR-574-3p (p = 0.017) as well as a significantly higher for hsa-miR-211-5p (p = 0.001) compared to the control group. Subgroup of women with early vs. late onset PE was characterized by significantly decreased expression level for hsa-miR-323-5p (p = 0.007) and overexpressed hsa-miR-30d-5p (p = 0.002), hsa-miR-5481 (p = 0.027). Conclusion. The noted multidirectional expression for some microRNAs in subgroups of PE patients confirms the validity for stratification of such pathology based on two distinct phenotypic manifestations (early and late forms) and indicates the existence of different pathophysiological vectors in PE formation. |
| format | Article |
| id | doaj-art-8c8365f0f8d44cb5a2c26e030d429d90 |
| institution | Kabale University |
| issn | 2313-7347 2500-3194 |
| language | Russian |
| publishDate | 2024-05-01 |
| publisher | IRBIS LLC |
| record_format | Article |
| series | Акушерство, гинекология и репродукция |
| spelling | doaj-art-8c8365f0f8d44cb5a2c26e030d429d902025-08-20T03:39:44ZrusIRBIS LLCАкушерство, гинекология и репродукция2313-73472500-31942024-05-0118216717910.17749/2313-7347/ob.gyn.rep.2024.483870Transcriptional profile features in patients with early and late preeclampsiaV. E.A. Kotelnikova0D. E. Pantyukhova1F. D. Ablyamitova2S. N. Vikinskaya3Kh. U. Khalilova4L. F. Mustafaeva5D. A. Barieva6D. V. Yarovaya7N. D. Chopik8M. S. Ermakova9L. E. Sorokina10Vernadsky Crimean Federal UniversityVernadsky Crimean Federal UniversityVernadsky Crimean Federal UniversityVernadsky Crimean Federal UniversityVernadsky Crimean Federal UniversityVernadsky Crimean Federal UniversityVernadsky Crimean Federal UniversityVernadsky Crimean Federal UniversityVernadsky Crimean Federal UniversityVernadsky Crimean Federal UniversityVernadsky Crimean Federal University; Medical center in Kolomenskoye CJSCAim: to assess the molecular mechanisms in developing various clinical phenotypes of preeclampsia (PE) by analyzing specific placental tissue transcriptome patterns. Materials and Methods. The prospective observational comparative study in parallel groups enrolled 43 pregnant women divided into 2 groups: main group – 23 pregnant women with diagnosed PE and control group – 20 apparently healthy women with uncomplicated pregnancy course, delivery and the postpartum period. To examine PE phenotypic features, the main group of pregnant women with PE was subsequently divided into 2 subgroups according to the date of pathology onset: early (n = 10) and late (n = 13) PE. Using the whole-genome next-generation sequencing (NGS), a comparative analysis of altered 18 microRNA level in placental tissue was carried out. Results. Pregnant women with early PE compared to the control group were characterized by significantly low expression level for hsa-miR-656-3p (p < 0.001), hsa-miR-323a-5p (p = 0.017), hsa-miR-519c-3p (p = 0.019), hsa-let-7i-5p (p = 0.019), hsa-miR-433-3p (p = 0.019), hsa-let-7g-5p (p = 0.030), hsa-miR-214-5p (p = 0.030), hsa-miR-27a-5p (p = 0.031), hsa-miR-339-5p (p = 0.041), hsa-miR-524-5p (p = 0.045), hsa-miR-1283 (p = 0.049) and high expression for hsa-miR-151a-5p (p = 0.007), hsa-miR-4521 (p = 0.018), hsa-miR-30d-5p (p = 0.026), hsa-miR-548l (p = 0.027), hsa-miR-133b (p = 0.034), hsa-miR-424-5p (p = 0.042), hsa-miR-211-5p (p = 0.049). Patients with late PE had significantly decreased expression for hsa-miR-656-3p (p = 0.050) andhsa-miR-574-3p (p = 0.017) as well as a significantly higher for hsa-miR-211-5p (p = 0.001) compared to the control group. Subgroup of women with early vs. late onset PE was characterized by significantly decreased expression level for hsa-miR-323-5p (p = 0.007) and overexpressed hsa-miR-30d-5p (p = 0.002), hsa-miR-5481 (p = 0.027). Conclusion. The noted multidirectional expression for some microRNAs in subgroups of PE patients confirms the validity for stratification of such pathology based on two distinct phenotypic manifestations (early and late forms) and indicates the existence of different pathophysiological vectors in PE formation.https://www.gynecology.su/jour/article/view/1966early and late preeclampsiaреplacentatranscriptomemicrorna |
| spellingShingle | V. E.A. Kotelnikova D. E. Pantyukhova F. D. Ablyamitova S. N. Vikinskaya Kh. U. Khalilova L. F. Mustafaeva D. A. Barieva D. V. Yarovaya N. D. Chopik M. S. Ermakova L. E. Sorokina Transcriptional profile features in patients with early and late preeclampsia Акушерство, гинекология и репродукция early and late preeclampsia ре placenta transcriptome microrna |
| title | Transcriptional profile features in patients with early and late preeclampsia |
| title_full | Transcriptional profile features in patients with early and late preeclampsia |
| title_fullStr | Transcriptional profile features in patients with early and late preeclampsia |
| title_full_unstemmed | Transcriptional profile features in patients with early and late preeclampsia |
| title_short | Transcriptional profile features in patients with early and late preeclampsia |
| title_sort | transcriptional profile features in patients with early and late preeclampsia |
| topic | early and late preeclampsia ре placenta transcriptome microrna |
| url | https://www.gynecology.su/jour/article/view/1966 |
| work_keys_str_mv | AT veakotelnikova transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia AT depantyukhova transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia AT fdablyamitova transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia AT snvikinskaya transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia AT khukhalilova transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia AT lfmustafaeva transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia AT dabarieva transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia AT dvyarovaya transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia AT ndchopik transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia AT msermakova transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia AT lesorokina transcriptionalprofilefeaturesinpatientswithearlyandlatepreeclampsia |