Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis?
Objectives. The aim of this study was to determine the level of five different pro- and anti-inflammatory cytokines to study the inflammatory response of leptospirosis. Materials and methods. The serum cytokine levels of IL-10, IL-17A, IL-21, IL-23, and TNF-α were investigated in 57 patients with le...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/9704532 |
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| _version_ | 1832561440600358912 |
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| author | Kanchana Bandara Chinthika Gunasekara Manjula Weerasekera Chamil Marasinghe Nilantha Ranasinghe Neluka Fernando |
| author_facet | Kanchana Bandara Chinthika Gunasekara Manjula Weerasekera Chamil Marasinghe Nilantha Ranasinghe Neluka Fernando |
| author_sort | Kanchana Bandara |
| collection | DOAJ |
| description | Objectives. The aim of this study was to determine the level of five different pro- and anti-inflammatory cytokines to study the inflammatory response of leptospirosis. Materials and methods. The serum cytokine levels of IL-10, IL-17A, IL-21, IL-23, and TNF-α were investigated in 57 patients with leptospirosis and 12 healthy controls using a commercially available ELISA kit (Mabtech, Sweden). Statistical analysis was done using Graphpad Prism. Results. Elevation of serum IL-10 and IL-17A levels and significant elevation of serum IL-21 (p=0.002), IL-23 (p=0.002), and TNF-α (p=0.039) were observed among leptospirosis patients compared to the healthy control group. The two major complications observed among these patients were renal failure and liver involvement. Renal failure was significantly associated with elevation of IL-21 and IL-23, while patients with liver involvement had a significant elevation of IL-21, IL-23, and TNF-α. Conclusion. Elevation of IL-17A together with the significant elevation of IL-21 and IL-23 suggests a possible involvement of Th17 cells in the immunopathogenesis of leptospirosis. |
| format | Article |
| id | doaj-art-8c73ed73d94c4095814723fe57649d2b |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-8c73ed73d94c4095814723fe57649d2b2025-02-03T01:24:58ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932018-01-01201810.1155/2018/97045329704532Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis?Kanchana Bandara0Chinthika Gunasekara1Manjula Weerasekera2Chamil Marasinghe3Nilantha Ranasinghe4Neluka Fernando5Department of Basic Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Ratmalana, Sri LankaDepartment of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri LankaDepartment of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri LankaDepartment of Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri LankaOut Patients Department, Base Hospital, Horana, Sri LankaDepartment of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri LankaObjectives. The aim of this study was to determine the level of five different pro- and anti-inflammatory cytokines to study the inflammatory response of leptospirosis. Materials and methods. The serum cytokine levels of IL-10, IL-17A, IL-21, IL-23, and TNF-α were investigated in 57 patients with leptospirosis and 12 healthy controls using a commercially available ELISA kit (Mabtech, Sweden). Statistical analysis was done using Graphpad Prism. Results. Elevation of serum IL-10 and IL-17A levels and significant elevation of serum IL-21 (p=0.002), IL-23 (p=0.002), and TNF-α (p=0.039) were observed among leptospirosis patients compared to the healthy control group. The two major complications observed among these patients were renal failure and liver involvement. Renal failure was significantly associated with elevation of IL-21 and IL-23, while patients with liver involvement had a significant elevation of IL-21, IL-23, and TNF-α. Conclusion. Elevation of IL-17A together with the significant elevation of IL-21 and IL-23 suggests a possible involvement of Th17 cells in the immunopathogenesis of leptospirosis.http://dx.doi.org/10.1155/2018/9704532 |
| spellingShingle | Kanchana Bandara Chinthika Gunasekara Manjula Weerasekera Chamil Marasinghe Nilantha Ranasinghe Neluka Fernando Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis? Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis? |
| title_full | Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis? |
| title_fullStr | Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis? |
| title_full_unstemmed | Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis? |
| title_short | Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis? |
| title_sort | do the th17 cells play a role in the pathogenesis of leptospirosis |
| url | http://dx.doi.org/10.1155/2018/9704532 |
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