Frugal method for extracting DNA from saliva suitable for low-budget labs and usable in PCR assays
Blood is the preferred source of DNA as it yields large quantities of high-quality DNA. However, there are challenges associated with this process, including difficulty in finding small and thin veins, rigid veins due to aging, and individuals with phlebotomy aversion, especially children. Therefore...
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| Format: | Article |
| Language: | English |
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Plovdiv University Press
2025-01-01
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| Series: | Journal of BioScience and Biotechnology |
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| Online Access: | https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/519/378 |
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| author | Kamar Shayah Joseph Shenekji Lana Karnoub Sana Wohbeh Hagar Hamsho |
| author_facet | Kamar Shayah Joseph Shenekji Lana Karnoub Sana Wohbeh Hagar Hamsho |
| author_sort | Kamar Shayah |
| collection | DOAJ |
| description | Blood is the preferred source of DNA as it yields large quantities of high-quality DNA. However, there are challenges associated with this process, including difficulty in finding small and thin veins, rigid veins due to aging, and individuals with phlebotomy aversion, especially children. Therefore, obtaining DNA from saliva is considered a suitable alternative that requires minimal resources and is usable in DNA assays without the need for a phlebotomist, at a lower cost compared to most DNA extraction kits that require expensive materials. The suggested frugal method relies on providing a new solution for extracting DNA from human saliva without the need for enzymes or high grade reagents or columns, which makes suitable for low budget laboratories in developing countries to manufacture it locally, it uses a phase separation with technical skills to extract the DNA from the saliva achieving safety by not using hazardous reagents, and it is a sustainable eco-friendly method that gives decent yield of good quality DNA in ~20 minutes to be used in PCR applications with a cost that is estimated to be 10 times cheaper than current used methods. |
| format | Article |
| id | doaj-art-8c5f6176de894f2a958b63de161048a2 |
| institution | Kabale University |
| issn | 1314-6238 1314-6246 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Plovdiv University Press |
| record_format | Article |
| series | Journal of BioScience and Biotechnology |
| spelling | doaj-art-8c5f6176de894f2a958b63de161048a22025-01-28T07:24:36ZengPlovdiv University PressJournal of BioScience and Biotechnology1314-62381314-62462025-01-0113212513110.69085/jbb20242125Frugal method for extracting DNA from saliva suitable for low-budget labs and usable in PCR assaysKamar Shayah0Joseph Shenekji1Lana Karnoub2Sana Wohbeh3Hagar Hamsho4University of Aleppo, Faculty of technical Engineering, Dept. of Biotechnology EngineeringUniversity of Aleppo, Faculty of technical Engineering, Dept. of Biotechnology EngineeringUniversity of Aleppo, Faculty of technical Engineering, Dept. of Biotechnology EngineeringUniversity of Aleppo, Faculty of technical Engineering, Dept. of Biotechnology EngineeringUniversity of Aleppo, Faculty of technical Engineering, Dept. of Biotechnology EngineeringBlood is the preferred source of DNA as it yields large quantities of high-quality DNA. However, there are challenges associated with this process, including difficulty in finding small and thin veins, rigid veins due to aging, and individuals with phlebotomy aversion, especially children. Therefore, obtaining DNA from saliva is considered a suitable alternative that requires minimal resources and is usable in DNA assays without the need for a phlebotomist, at a lower cost compared to most DNA extraction kits that require expensive materials. The suggested frugal method relies on providing a new solution for extracting DNA from human saliva without the need for enzymes or high grade reagents or columns, which makes suitable for low budget laboratories in developing countries to manufacture it locally, it uses a phase separation with technical skills to extract the DNA from the saliva achieving safety by not using hazardous reagents, and it is a sustainable eco-friendly method that gives decent yield of good quality DNA in ~20 minutes to be used in PCR applications with a cost that is estimated to be 10 times cheaper than current used methods.https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/519/378dna extractionsalivasustainablefrugaleconomicpcr |
| spellingShingle | Kamar Shayah Joseph Shenekji Lana Karnoub Sana Wohbeh Hagar Hamsho Frugal method for extracting DNA from saliva suitable for low-budget labs and usable in PCR assays Journal of BioScience and Biotechnology dna extraction saliva sustainable frugal economic pcr |
| title | Frugal method for extracting DNA from saliva suitable for low-budget labs and usable in PCR assays |
| title_full | Frugal method for extracting DNA from saliva suitable for low-budget labs and usable in PCR assays |
| title_fullStr | Frugal method for extracting DNA from saliva suitable for low-budget labs and usable in PCR assays |
| title_full_unstemmed | Frugal method for extracting DNA from saliva suitable for low-budget labs and usable in PCR assays |
| title_short | Frugal method for extracting DNA from saliva suitable for low-budget labs and usable in PCR assays |
| title_sort | frugal method for extracting dna from saliva suitable for low budget labs and usable in pcr assays |
| topic | dna extraction saliva sustainable frugal economic pcr |
| url | https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/519/378 |
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