circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression
Objective. This study is aimed at exploring the influence of circular RNA- (circRNA-) RANGAP1 targeting microRNA- (miR-) 542-3p/myosin regulatory light chain interacting protein (MYLIP) on the biological function of osteosarcoma (OS) cells. Methods. Tumor tissues and normal tissues were collected fr...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Applied Bionics and Biomechanics |
| Online Access: | http://dx.doi.org/10.1155/2022/4247670 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850213072462938112 |
|---|---|
| author | Jundong Sheng Jin Liu Junwang Du Yongping Wang |
| author_facet | Jundong Sheng Jin Liu Junwang Du Yongping Wang |
| author_sort | Jundong Sheng |
| collection | DOAJ |
| description | Objective. This study is aimed at exploring the influence of circular RNA- (circRNA-) RANGAP1 targeting microRNA- (miR-) 542-3p/myosin regulatory light chain interacting protein (MYLIP) on the biological function of osteosarcoma (OS) cells. Methods. Tumor tissues and normal tissues were collected from OS patients and circ-RANGAP1, miR-542-3p, and MYLIP expression was tested by RT-qPCR. The correlation between the clinicopathology/prognosis of patients with OS and circ-RANGAP1 expression was observed. Human OS cell line MG-63 was screened to determine the influences of circ-RANGAP1 and miR-542-3p on OS cell progression. The targeting relation of circ-RANGAP1, miR-542-3p, and MYLIP was probed. Results. circ-RANGAP1 expression was elevated in tumor tissues from OS patients, which was correlated to the poor clinicopathology. circ-RANGAP1 expression was augmented in males or patients younger than 20 years old or patients with advanced OS. Higher circ-RANGAP1 expression indicated a poor prognosis in OS patients. After silencing circ-RANGAP1 or elevating miR-542-3p in MG63 cells, cell progression was limited. miR-542-3p downregulation reduced the therapeutic efficacy of silenced circ-RANGAP1. circ-RANGAP1 bound with miR-542-3p to target MYLIP. Conclusion. Silenced circ-RANGAP1 boosts MYLIP expression via competitive binding of miR-542-3p to facilitate OS cell progression. |
| format | Article |
| id | doaj-art-8c2f5b84d03f4e7ebfcd2fe11f6fb1c4 |
| institution | OA Journals |
| issn | 1754-2103 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applied Bionics and Biomechanics |
| spelling | doaj-art-8c2f5b84d03f4e7ebfcd2fe11f6fb1c42025-08-20T02:09:12ZengWileyApplied Bionics and Biomechanics1754-21032022-01-01202210.1155/2022/4247670circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell ProgressionJundong Sheng0Jin Liu1Junwang Du2Yongping Wang3Department of OrthopedicsDepartment of OrthopedicsDepartment of AnesthesiologyDepartment of OrthopedicsObjective. This study is aimed at exploring the influence of circular RNA- (circRNA-) RANGAP1 targeting microRNA- (miR-) 542-3p/myosin regulatory light chain interacting protein (MYLIP) on the biological function of osteosarcoma (OS) cells. Methods. Tumor tissues and normal tissues were collected from OS patients and circ-RANGAP1, miR-542-3p, and MYLIP expression was tested by RT-qPCR. The correlation between the clinicopathology/prognosis of patients with OS and circ-RANGAP1 expression was observed. Human OS cell line MG-63 was screened to determine the influences of circ-RANGAP1 and miR-542-3p on OS cell progression. The targeting relation of circ-RANGAP1, miR-542-3p, and MYLIP was probed. Results. circ-RANGAP1 expression was elevated in tumor tissues from OS patients, which was correlated to the poor clinicopathology. circ-RANGAP1 expression was augmented in males or patients younger than 20 years old or patients with advanced OS. Higher circ-RANGAP1 expression indicated a poor prognosis in OS patients. After silencing circ-RANGAP1 or elevating miR-542-3p in MG63 cells, cell progression was limited. miR-542-3p downregulation reduced the therapeutic efficacy of silenced circ-RANGAP1. circ-RANGAP1 bound with miR-542-3p to target MYLIP. Conclusion. Silenced circ-RANGAP1 boosts MYLIP expression via competitive binding of miR-542-3p to facilitate OS cell progression.http://dx.doi.org/10.1155/2022/4247670 |
| spellingShingle | Jundong Sheng Jin Liu Junwang Du Yongping Wang circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression Applied Bionics and Biomechanics |
| title | circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression |
| title_full | circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression |
| title_fullStr | circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression |
| title_full_unstemmed | circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression |
| title_short | circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression |
| title_sort | circ rangap1 microrna 542 3p myosin regulatory light chain interacting protein axis modulates the osteosarcoma cell progression |
| url | http://dx.doi.org/10.1155/2022/4247670 |
| work_keys_str_mv | AT jundongsheng circrangap1microrna5423pmyosinregulatorylightchaininteractingproteinaxismodulatestheosteosarcomacellprogression AT jinliu circrangap1microrna5423pmyosinregulatorylightchaininteractingproteinaxismodulatestheosteosarcomacellprogression AT junwangdu circrangap1microrna5423pmyosinregulatorylightchaininteractingproteinaxismodulatestheosteosarcomacellprogression AT yongpingwang circrangap1microrna5423pmyosinregulatorylightchaininteractingproteinaxismodulatestheosteosarcomacellprogression |