Identification of candidate genes for leaf size by QTL mapping and transcriptome sequencing in Brassica napus L
Abstract Leaf is the main photosynthetic organ at the seedling stage of rapeseed and leaf size is a crucial agronomic trait affecting rapeseed yield. Understanding the genetic mechanisms underlying leaf size is therefore important for rapeseed breeding. In this study, QTL mapping for three traits re...
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2025-01-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-025-11205-9 |
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| author | Fengjie Cheng Yuwen Wang Aoyi Peng Shuyu Li Jun Chen Xiaoxiao Zheng Jie Xiong Ge Ding Bingchao Zhang Wen Zhai Laiqiang Song Wenliang Wei Lunlin Chen |
| author_facet | Fengjie Cheng Yuwen Wang Aoyi Peng Shuyu Li Jun Chen Xiaoxiao Zheng Jie Xiong Ge Ding Bingchao Zhang Wen Zhai Laiqiang Song Wenliang Wei Lunlin Chen |
| author_sort | Fengjie Cheng |
| collection | DOAJ |
| description | Abstract Leaf is the main photosynthetic organ at the seedling stage of rapeseed and leaf size is a crucial agronomic trait affecting rapeseed yield. Understanding the genetic mechanisms underlying leaf size is therefore important for rapeseed breeding. In this study, QTL mapping for three traits related to leaf size, i.e., leaf width (LW), leaf length (LL) and leaf area (LA), was performed using a recombinant inbred line (RIL) population and four QTLs for LW, two QTLs for LL and four QTLs for LA were detected. Transcriptome analysis revealed that differentially expressed genes (DEGs) were enriched in the GO terms related to microtubules, and the expression level of several genes involved in cell division also showed significant differences. Microscopic analysis suggested that the cell number was the main factor regulating leaf size. Combining QTL mapping and RNA sequencing, four promising candidate genes, including BnaA10G0085600ZS, BnaA10G0156900ZS, BnaC03G0441700ZS, and BnaC08G0410600ZS, were proposed to control leaf size by regulating cell division. The results of QTL, transcriptome analysis, and anatomical observation were highly consistent, collectively revealing that genes related to cell division played a crucial role in regulating the leaf size traits in rapeseed. These findings provided further insights into the genetic mechanism of leaf size and built fundamental theory basis for high-density tolerance breeding in rapeseed. |
| format | Article |
| id | doaj-art-8bf69a447c4145969d255e9babf27a04 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-8bf69a447c4145969d255e9babf27a042025-01-19T12:11:29ZengBMCBMC Genomics1471-21642025-01-0126111110.1186/s12864-025-11205-9Identification of candidate genes for leaf size by QTL mapping and transcriptome sequencing in Brassica napus LFengjie Cheng0Yuwen Wang1Aoyi Peng2Shuyu Li3Jun Chen4Xiaoxiao Zheng5Jie Xiong6Ge Ding7Bingchao Zhang8Wen Zhai9Laiqiang Song10Wenliang Wei11Lunlin Chen12College of Agriculture, Yangtze UniversityCrop Institute, Jiangxi Academy of Agricultural SciencesCollege of Agriculture, Yangtze UniversityCrop Institute, Jiangxi Academy of Agricultural SciencesEast China University of TechnologyCrop Institute, Jiangxi Academy of Agricultural SciencesCrop Institute, Jiangxi Academy of Agricultural SciencesCrop Institute, Jiangxi Academy of Agricultural SciencesCrop Institute, Jiangxi Academy of Agricultural SciencesEast China University of TechnologyCrop Institute, Jiangxi Academy of Agricultural SciencesCollege of Agriculture, Yangtze UniversityCrop Institute, Jiangxi Academy of Agricultural SciencesAbstract Leaf is the main photosynthetic organ at the seedling stage of rapeseed and leaf size is a crucial agronomic trait affecting rapeseed yield. Understanding the genetic mechanisms underlying leaf size is therefore important for rapeseed breeding. In this study, QTL mapping for three traits related to leaf size, i.e., leaf width (LW), leaf length (LL) and leaf area (LA), was performed using a recombinant inbred line (RIL) population and four QTLs for LW, two QTLs for LL and four QTLs for LA were detected. Transcriptome analysis revealed that differentially expressed genes (DEGs) were enriched in the GO terms related to microtubules, and the expression level of several genes involved in cell division also showed significant differences. Microscopic analysis suggested that the cell number was the main factor regulating leaf size. Combining QTL mapping and RNA sequencing, four promising candidate genes, including BnaA10G0085600ZS, BnaA10G0156900ZS, BnaC03G0441700ZS, and BnaC08G0410600ZS, were proposed to control leaf size by regulating cell division. The results of QTL, transcriptome analysis, and anatomical observation were highly consistent, collectively revealing that genes related to cell division played a crucial role in regulating the leaf size traits in rapeseed. These findings provided further insights into the genetic mechanism of leaf size and built fundamental theory basis for high-density tolerance breeding in rapeseed.https://doi.org/10.1186/s12864-025-11205-9Leaf widthLeaf lengthLeaf areaQTL mappingTranscriptome sequencingBrassica napus L |
| spellingShingle | Fengjie Cheng Yuwen Wang Aoyi Peng Shuyu Li Jun Chen Xiaoxiao Zheng Jie Xiong Ge Ding Bingchao Zhang Wen Zhai Laiqiang Song Wenliang Wei Lunlin Chen Identification of candidate genes for leaf size by QTL mapping and transcriptome sequencing in Brassica napus L BMC Genomics Leaf width Leaf length Leaf area QTL mapping Transcriptome sequencing Brassica napus L |
| title | Identification of candidate genes for leaf size by QTL mapping and transcriptome sequencing in Brassica napus L |
| title_full | Identification of candidate genes for leaf size by QTL mapping and transcriptome sequencing in Brassica napus L |
| title_fullStr | Identification of candidate genes for leaf size by QTL mapping and transcriptome sequencing in Brassica napus L |
| title_full_unstemmed | Identification of candidate genes for leaf size by QTL mapping and transcriptome sequencing in Brassica napus L |
| title_short | Identification of candidate genes for leaf size by QTL mapping and transcriptome sequencing in Brassica napus L |
| title_sort | identification of candidate genes for leaf size by qtl mapping and transcriptome sequencing in brassica napus l |
| topic | Leaf width Leaf length Leaf area QTL mapping Transcriptome sequencing Brassica napus L |
| url | https://doi.org/10.1186/s12864-025-11205-9 |
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