Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton
Abstract Bacterial transcription activator-like effectors (TALEs) promote pathogenicity by activating host susceptibility (S) genes. To understand the pathogenicity and host adaptation of Xanthomonas citri pv. malvacearum (Xcm), we assemble the genome and the TALE repertoire of three recent Xcm Texa...
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-55926-7 |
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| author | Brendan W. Mormile Yan Yan Taran Bauer Li Wang Rachel C. Rivero Sara C. D. Carpenter Catherine Danmaigona Clement Kevin L. Cox Lin Zhang Xiyu Ma Terry A. Wheeler Jane K. Dever Ping He Adam J. Bogdanove Libo Shan |
| author_facet | Brendan W. Mormile Yan Yan Taran Bauer Li Wang Rachel C. Rivero Sara C. D. Carpenter Catherine Danmaigona Clement Kevin L. Cox Lin Zhang Xiyu Ma Terry A. Wheeler Jane K. Dever Ping He Adam J. Bogdanove Libo Shan |
| author_sort | Brendan W. Mormile |
| collection | DOAJ |
| description | Abstract Bacterial transcription activator-like effectors (TALEs) promote pathogenicity by activating host susceptibility (S) genes. To understand the pathogenicity and host adaptation of Xanthomonas citri pv. malvacearum (Xcm), we assemble the genome and the TALE repertoire of three recent Xcm Texas isolates. A newly evolved TALE, Tal7b, activates GhSWEET14a and GhSWEET14b, different from GhSWEET10 targeted by a TALE in an early Xcm isolate. Activation of GhSWEET14a and GhSWEET14b results in water-soaked lesions. Transcriptome profiling coupled with TALE-binding element prediction identify a pectin lyase gene as an additional Tal7b target, quantitatively contributing to Xcm virulence alongside GhSWEET14a/b. CRISPR-Cas9 gene editing supports the function of GhSWEETs in cotton bacterial blight and the promise of disrupting the TALE-binding site in S genes for disease management. Collectively, our findings elucidate the rapid evolution of TALEs in Xanthomonas field isolates and highlight the virulence mechanism wherein TALEs induce multiple S genes to promote pathogenicity. |
| format | Article |
| id | doaj-art-8be68930dcbd4c37b0e788df5fa121c8 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-8be68930dcbd4c37b0e788df5fa121c82025-01-19T12:30:02ZengNature PortfolioNature Communications2041-17232025-01-0116111710.1038/s41467-025-55926-7Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cottonBrendan W. Mormile0Yan Yan1Taran Bauer2Li Wang3Rachel C. Rivero4Sara C. D. Carpenter5Catherine Danmaigona Clement6Kevin L. Cox7Lin Zhang8Xiyu Ma9Terry A. Wheeler10Jane K. Dever11Ping He12Adam J. Bogdanove13Libo Shan14Department of Molecular, Cellular, and Developmental Biology, University of MichiganDepartment of Molecular, Cellular, and Developmental Biology, University of MichiganPlant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell UniversityPlant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell UniversityDepartment of Molecular, Cellular, and Developmental Biology, University of MichiganPlant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell UniversityDepartment of Plant Pathology and Microbiology, Texas A&M UniversityDepartment of Plant Pathology and Microbiology, Texas A&M UniversityDepartment of Plant Pathology and Microbiology, Texas A&M UniversityDepartment of Plant Pathology and Microbiology, Texas A&M UniversityTexas A&M AgriLife ResearchTexas A&M AgriLife ResearchDepartment of Molecular, Cellular, and Developmental Biology, University of MichiganPlant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell UniversityDepartment of Molecular, Cellular, and Developmental Biology, University of MichiganAbstract Bacterial transcription activator-like effectors (TALEs) promote pathogenicity by activating host susceptibility (S) genes. To understand the pathogenicity and host adaptation of Xanthomonas citri pv. malvacearum (Xcm), we assemble the genome and the TALE repertoire of three recent Xcm Texas isolates. A newly evolved TALE, Tal7b, activates GhSWEET14a and GhSWEET14b, different from GhSWEET10 targeted by a TALE in an early Xcm isolate. Activation of GhSWEET14a and GhSWEET14b results in water-soaked lesions. Transcriptome profiling coupled with TALE-binding element prediction identify a pectin lyase gene as an additional Tal7b target, quantitatively contributing to Xcm virulence alongside GhSWEET14a/b. CRISPR-Cas9 gene editing supports the function of GhSWEETs in cotton bacterial blight and the promise of disrupting the TALE-binding site in S genes for disease management. Collectively, our findings elucidate the rapid evolution of TALEs in Xanthomonas field isolates and highlight the virulence mechanism wherein TALEs induce multiple S genes to promote pathogenicity.https://doi.org/10.1038/s41467-025-55926-7 |
| spellingShingle | Brendan W. Mormile Yan Yan Taran Bauer Li Wang Rachel C. Rivero Sara C. D. Carpenter Catherine Danmaigona Clement Kevin L. Cox Lin Zhang Xiyu Ma Terry A. Wheeler Jane K. Dever Ping He Adam J. Bogdanove Libo Shan Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton Nature Communications |
| title | Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton |
| title_full | Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton |
| title_fullStr | Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton |
| title_full_unstemmed | Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton |
| title_short | Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton |
| title_sort | activation of three targets by a tal effector confers susceptibility to bacterial blight of cotton |
| url | https://doi.org/10.1038/s41467-025-55926-7 |
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