Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice
Summary: Activatable photoacoustic imaging probes offer a strategy to efficiently reduce background noise from endogenous chromophores. We present a protocol for tumor imaging in mice using an activatable covalent photoacoustic imaging probe, NOx-JS013. We describe steps for synthesizing NOx-JS013,...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725004526 |
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| _version_ | 1849226878199529472 |
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| author | Jiho Song Tianqu Zhai Janggun Jo Jae Won Chang |
| author_facet | Jiho Song Tianqu Zhai Janggun Jo Jae Won Chang |
| author_sort | Jiho Song |
| collection | DOAJ |
| description | Summary: Activatable photoacoustic imaging probes offer a strategy to efficiently reduce background noise from endogenous chromophores. We present a protocol for tumor imaging in mice using an activatable covalent photoacoustic imaging probe, NOx-JS013. We describe steps for synthesizing NOx-JS013, in vitro and in situ validation through gel-based activity-based protein profiling and cellular imaging, and tumor imaging of aggressive prostate cancer mouse models. This approach provides a strategy for mitigating background noise in the development of photoacoustic imaging probes.For complete details on the use and execution of this protocol, please refer to Song et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-8bd041ed66c644ceb24f97e541b6bfb7 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-8bd041ed66c644ceb24f97e541b6bfb72025-08-24T05:14:35ZengElsevierSTAR Protocols2666-16672025-09-016310404610.1016/j.xpro.2025.104046Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in miceJiho Song0Tianqu Zhai1Janggun Jo2Jae Won Chang3Department of Pharmacology and Chemical Biology, Emory University, Atlanta, GA 30322, USA; Corresponding authorDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USADepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Corresponding authorDepartment of Pharmacology and Chemical Biology, Emory University, Atlanta, GA 30322, USA; Department of Hematology and Medical Oncology, Emory University, Atlanta, GA 30322, USA; Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA; Corresponding authorSummary: Activatable photoacoustic imaging probes offer a strategy to efficiently reduce background noise from endogenous chromophores. We present a protocol for tumor imaging in mice using an activatable covalent photoacoustic imaging probe, NOx-JS013. We describe steps for synthesizing NOx-JS013, in vitro and in situ validation through gel-based activity-based protein profiling and cellular imaging, and tumor imaging of aggressive prostate cancer mouse models. This approach provides a strategy for mitigating background noise in the development of photoacoustic imaging probes.For complete details on the use and execution of this protocol, please refer to Song et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725004526cancermicroscopyMolecular/Chemical probes |
| spellingShingle | Jiho Song Tianqu Zhai Janggun Jo Jae Won Chang Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice STAR Protocols cancer microscopy Molecular/Chemical probes |
| title | Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice |
| title_full | Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice |
| title_fullStr | Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice |
| title_full_unstemmed | Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice |
| title_short | Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice |
| title_sort | protocol for covalent targeted and activatable photoacoustic imaging agent for tumor imaging in mice |
| topic | cancer microscopy Molecular/Chemical probes |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725004526 |
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