Galectin-10 Is Released in the Nasal Lavage Fluid of Patients with Aspirin-Sensitive Respiratory Disease
The aim of this work was to determine the presence of galectin-10 in nasal lavage fluid (NLF) of patients with aspirin-sensitive respiratory disease (ASRD) before and after challenge with L-ASA (aspirin) by ELISA. Fifteen ASRD patients, ten aspirin-tolerant asthmatics (ATA), and fifteen healthy con...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/474020 |
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| author | Ma. Cristina Negrete-Garcia Carla Yoneli Jiménez-Torres Noe Alvarado-Vásquez A. Rosalía Montes-Vizuet J. R. Velázquez-Rodriguez M. Carmen Jimenez-Martinez Luis Manuel Teran-Juárez |
| author_facet | Ma. Cristina Negrete-Garcia Carla Yoneli Jiménez-Torres Noe Alvarado-Vásquez A. Rosalía Montes-Vizuet J. R. Velázquez-Rodriguez M. Carmen Jimenez-Martinez Luis Manuel Teran-Juárez |
| author_sort | Ma. Cristina Negrete-Garcia |
| collection | DOAJ |
| description | The aim of this work was to determine the presence of galectin-10 in nasal lavage fluid (NLF) of patients with aspirin-sensitive respiratory disease (ASRD) before and after challenge with L-ASA (aspirin) by ELISA. Fifteen ASRD patients, ten aspirin-tolerant asthmatics (ATA), and fifteen healthy controls (HC) were studied. The baseline presence of Galectin-10 in PBMC was determined using real time PCR. Galectin-10 was evaluated in tissue of nasal polyps by western blot. Our results showed a lower expression in PBMC of ASRD patients than in ATA and healthy controls. However, a higher concentration of galectin-10 in NLF was found in ASRD patients before and after L-ASA challenge; western blot confirmed a high expression of galectin-10 in tissue from nasal polyps obtained from ASRD patients. Our results suggest a probable role of galectin-10 in the inflammatory response observed in ASRD patients; however, confirmatory studies are needed. |
| format | Article |
| id | doaj-art-8bca24563b744810b2a4417489ba4e6e |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-8bca24563b744810b2a4417489ba4e6e2025-02-03T01:30:45ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/474020474020Galectin-10 Is Released in the Nasal Lavage Fluid of Patients with Aspirin-Sensitive Respiratory DiseaseMa. Cristina Negrete-Garcia0Carla Yoneli Jiménez-Torres1Noe Alvarado-Vásquez2A. Rosalía Montes-Vizuet3J. R. Velázquez-Rodriguez4M. Carmen Jimenez-Martinez5Luis Manuel Teran-Juárez6Department of Immunogenetics and Allergy, Instituto Nacional de Enfermedades Respiratorias, Calzada Tlalpan 4502, 14080 Mexico, DF, MexicoDepartment of Immunogenetics and Allergy, Instituto Nacional de Enfermedades Respiratorias, Calzada Tlalpan 4502, 14080 Mexico, DF, MexicoDepartment of Biochemistry, Instituto Nacional de Enfermedades Respiratorias, Calzada Tlalpan 4502, 14080 Mexico, DF, MexicoDepartment of Immunogenetics and Allergy, Instituto Nacional de Enfermedades Respiratorias, Calzada Tlalpan 4502, 14080 Mexico, DF, MexicoDepartment of Immunogenetics and Allergy, Instituto Nacional de Enfermedades Respiratorias, Calzada Tlalpan 4502, 14080 Mexico, DF, MexicoDepartment of Immunology, Research Unit, Institute of Ophthalmology, Foundation Conde de Valenciana, 06800 Mexico, DF, MexicoDepartment of Immunogenetics and Allergy, Instituto Nacional de Enfermedades Respiratorias, Calzada Tlalpan 4502, 14080 Mexico, DF, MexicoThe aim of this work was to determine the presence of galectin-10 in nasal lavage fluid (NLF) of patients with aspirin-sensitive respiratory disease (ASRD) before and after challenge with L-ASA (aspirin) by ELISA. Fifteen ASRD patients, ten aspirin-tolerant asthmatics (ATA), and fifteen healthy controls (HC) were studied. The baseline presence of Galectin-10 in PBMC was determined using real time PCR. Galectin-10 was evaluated in tissue of nasal polyps by western blot. Our results showed a lower expression in PBMC of ASRD patients than in ATA and healthy controls. However, a higher concentration of galectin-10 in NLF was found in ASRD patients before and after L-ASA challenge; western blot confirmed a high expression of galectin-10 in tissue from nasal polyps obtained from ASRD patients. Our results suggest a probable role of galectin-10 in the inflammatory response observed in ASRD patients; however, confirmatory studies are needed.http://dx.doi.org/10.1100/2012/474020 |
| spellingShingle | Ma. Cristina Negrete-Garcia Carla Yoneli Jiménez-Torres Noe Alvarado-Vásquez A. Rosalía Montes-Vizuet J. R. Velázquez-Rodriguez M. Carmen Jimenez-Martinez Luis Manuel Teran-Juárez Galectin-10 Is Released in the Nasal Lavage Fluid of Patients with Aspirin-Sensitive Respiratory Disease The Scientific World Journal |
| title | Galectin-10 Is Released in the Nasal Lavage Fluid of Patients with Aspirin-Sensitive Respiratory Disease |
| title_full | Galectin-10 Is Released in the Nasal Lavage Fluid of Patients with Aspirin-Sensitive Respiratory Disease |
| title_fullStr | Galectin-10 Is Released in the Nasal Lavage Fluid of Patients with Aspirin-Sensitive Respiratory Disease |
| title_full_unstemmed | Galectin-10 Is Released in the Nasal Lavage Fluid of Patients with Aspirin-Sensitive Respiratory Disease |
| title_short | Galectin-10 Is Released in the Nasal Lavage Fluid of Patients with Aspirin-Sensitive Respiratory Disease |
| title_sort | galectin 10 is released in the nasal lavage fluid of patients with aspirin sensitive respiratory disease |
| url | http://dx.doi.org/10.1100/2012/474020 |
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