Investigating the Pulmonary Host Response of <i>Acinetobacter baumannii</i> Infection-Associated Pneumonia by Metagenomic Next-Generation Sequencing
<b>Background:</b> For investigating the host response in <i>Acinetobacter baumannii</i> associated pneumonia, we analyzed the host genetic sequences obtained from metagenomic next-generation sequencing (mNGS). <b>Methods:</b> The samples for mNGS were bronchoalve...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/13/1/142 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588988800565248 |
|---|---|
| author | Mu-Jung Chou Chih-Hung Cheng Hui-Ching Wang Ming-Ju Tsai Chau-Chyun Sheu Wei-An Chang |
| author_facet | Mu-Jung Chou Chih-Hung Cheng Hui-Ching Wang Ming-Ju Tsai Chau-Chyun Sheu Wei-An Chang |
| author_sort | Mu-Jung Chou |
| collection | DOAJ |
| description | <b>Background:</b> For investigating the host response in <i>Acinetobacter baumannii</i> associated pneumonia, we analyzed the host genetic sequences obtained from metagenomic next-generation sequencing (mNGS). <b>Methods:</b> The samples for mNGS were bronchoalveolar lavage fluid (BALF) collected from the lungs of patients infected with <i>A. baumannii</i> and from patients without bacterial infections. BALF samples from patients with pneumonia were collected from the lungs of patients infected with <i>A. baumannii</i> with New Delhi metallo-β-lactamase (NDM, before treatment), A. baumannii with NDM (post-treatment), <i>A. baumannii</i> without resistant genes, and those without bacterial infection. Partek was used for investigating enriched functions and pathways related to the pulmonary host response to pneumonia caused by <i>A. baumannii</i> with NDM infection and <i>A. baumannii</i> without antimicrobial-resistant genes. The STRING was employed for identifying protein interaction pathways related to the pulmonary host response to pneumonia caused by <i>A. baumannii</i> without antimicrobial-resistant genes. <b>Results:</b> In pulmonary host response to pneumonia caused by <i>A. baumannii</i> with NDM, five immune system-related pathways and five pathways related to signal transduction were identified. No significant differences were observed in the immune system and signal transduction pathways in the pulmonary host response to pneumonia caused by <i>A. baumannii</i> without antimicrobial-resistant genes. However, significant differences were noted in the phagosome, ferroptosis, and regulation of the actin cytoskeleton in cellular processes. <b>Conclusions:</b> mNGS provides information not only on pathogen gene expression but also on host gene expression. In this study, we found that pneumonia with <i>A. baumannii</i> carrying the NDM resistance gene triggers stronger immune responses in the lung, while pneumonia with <i>A. baumannii</i> lacking antimicrobial resistance genes is more linked to iron-related pathways. |
| format | Article |
| id | doaj-art-8bbaf6b0ba464b079d8cd598f5b5bc1e |
| institution | Kabale University |
| issn | 2227-9059 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj-art-8bbaf6b0ba464b079d8cd598f5b5bc1e2025-01-24T13:24:09ZengMDPI AGBiomedicines2227-90592025-01-0113114210.3390/biomedicines13010142Investigating the Pulmonary Host Response of <i>Acinetobacter baumannii</i> Infection-Associated Pneumonia by Metagenomic Next-Generation SequencingMu-Jung Chou0Chih-Hung Cheng1Hui-Ching Wang2Ming-Ju Tsai3Chau-Chyun Sheu4Wei-An Chang5Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Nursing, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan<b>Background:</b> For investigating the host response in <i>Acinetobacter baumannii</i> associated pneumonia, we analyzed the host genetic sequences obtained from metagenomic next-generation sequencing (mNGS). <b>Methods:</b> The samples for mNGS were bronchoalveolar lavage fluid (BALF) collected from the lungs of patients infected with <i>A. baumannii</i> and from patients without bacterial infections. BALF samples from patients with pneumonia were collected from the lungs of patients infected with <i>A. baumannii</i> with New Delhi metallo-β-lactamase (NDM, before treatment), A. baumannii with NDM (post-treatment), <i>A. baumannii</i> without resistant genes, and those without bacterial infection. Partek was used for investigating enriched functions and pathways related to the pulmonary host response to pneumonia caused by <i>A. baumannii</i> with NDM infection and <i>A. baumannii</i> without antimicrobial-resistant genes. The STRING was employed for identifying protein interaction pathways related to the pulmonary host response to pneumonia caused by <i>A. baumannii</i> without antimicrobial-resistant genes. <b>Results:</b> In pulmonary host response to pneumonia caused by <i>A. baumannii</i> with NDM, five immune system-related pathways and five pathways related to signal transduction were identified. No significant differences were observed in the immune system and signal transduction pathways in the pulmonary host response to pneumonia caused by <i>A. baumannii</i> without antimicrobial-resistant genes. However, significant differences were noted in the phagosome, ferroptosis, and regulation of the actin cytoskeleton in cellular processes. <b>Conclusions:</b> mNGS provides information not only on pathogen gene expression but also on host gene expression. In this study, we found that pneumonia with <i>A. baumannii</i> carrying the NDM resistance gene triggers stronger immune responses in the lung, while pneumonia with <i>A. baumannii</i> lacking antimicrobial resistance genes is more linked to iron-related pathways.https://www.mdpi.com/2227-9059/13/1/142mNGS<i>Acinetobacter baumannii</i>NDMimmunepulmonary host responseferroptosis |
| spellingShingle | Mu-Jung Chou Chih-Hung Cheng Hui-Ching Wang Ming-Ju Tsai Chau-Chyun Sheu Wei-An Chang Investigating the Pulmonary Host Response of <i>Acinetobacter baumannii</i> Infection-Associated Pneumonia by Metagenomic Next-Generation Sequencing Biomedicines mNGS <i>Acinetobacter baumannii</i> NDM immune pulmonary host response ferroptosis |
| title | Investigating the Pulmonary Host Response of <i>Acinetobacter baumannii</i> Infection-Associated Pneumonia by Metagenomic Next-Generation Sequencing |
| title_full | Investigating the Pulmonary Host Response of <i>Acinetobacter baumannii</i> Infection-Associated Pneumonia by Metagenomic Next-Generation Sequencing |
| title_fullStr | Investigating the Pulmonary Host Response of <i>Acinetobacter baumannii</i> Infection-Associated Pneumonia by Metagenomic Next-Generation Sequencing |
| title_full_unstemmed | Investigating the Pulmonary Host Response of <i>Acinetobacter baumannii</i> Infection-Associated Pneumonia by Metagenomic Next-Generation Sequencing |
| title_short | Investigating the Pulmonary Host Response of <i>Acinetobacter baumannii</i> Infection-Associated Pneumonia by Metagenomic Next-Generation Sequencing |
| title_sort | investigating the pulmonary host response of i acinetobacter baumannii i infection associated pneumonia by metagenomic next generation sequencing |
| topic | mNGS <i>Acinetobacter baumannii</i> NDM immune pulmonary host response ferroptosis |
| url | https://www.mdpi.com/2227-9059/13/1/142 |
| work_keys_str_mv | AT mujungchou investigatingthepulmonaryhostresponseofiacinetobacterbaumanniiiinfectionassociatedpneumoniabymetagenomicnextgenerationsequencing AT chihhungcheng investigatingthepulmonaryhostresponseofiacinetobacterbaumanniiiinfectionassociatedpneumoniabymetagenomicnextgenerationsequencing AT huichingwang investigatingthepulmonaryhostresponseofiacinetobacterbaumanniiiinfectionassociatedpneumoniabymetagenomicnextgenerationsequencing AT mingjutsai investigatingthepulmonaryhostresponseofiacinetobacterbaumanniiiinfectionassociatedpneumoniabymetagenomicnextgenerationsequencing AT chauchyunsheu investigatingthepulmonaryhostresponseofiacinetobacterbaumanniiiinfectionassociatedpneumoniabymetagenomicnextgenerationsequencing AT weianchang investigatingthepulmonaryhostresponseofiacinetobacterbaumanniiiinfectionassociatedpneumoniabymetagenomicnextgenerationsequencing |