Nanosensor-based imaging of realtime dopamine release in neurons derived from iPSCs of patients with Parkinson's disease
Dopamine (DA) is an essential neuromodulator that underlies critical aspects of cognitive processes, motor function, and reward systems. Disruptions in DA signaling contribute to various neurodegenerative diseases, including Parkinson's disease (PD). Despite its important role in neuronal funct...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425000432 |
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| author | Nayeon Lee Dakyeon Lee Jae Hyeok Lee Bo Seok Lee Sungjee Kim Jae Ho Kim Sanghwa Jeong |
| author_facet | Nayeon Lee Dakyeon Lee Jae Hyeok Lee Bo Seok Lee Sungjee Kim Jae Ho Kim Sanghwa Jeong |
| author_sort | Nayeon Lee |
| collection | DOAJ |
| description | Dopamine (DA) is an essential neuromodulator that underlies critical aspects of cognitive processes, motor function, and reward systems. Disruptions in DA signaling contribute to various neurodegenerative diseases, including Parkinson's disease (PD). Despite its important role in neuronal function, the impact of DA release/uptake on neurochemical imbalances during neuronal development remains unclear. We propose a novel application of near-infrared catecholamine nanosensor (NIRCat) for real-time visualization of DA neurotransmission among neurodegenerative disease cells. The near-infrared fluorescence (900–1400 nm) of NIRCat allows the semi-quantitative measurement of DA release in living neurons and offers insights into cellular dynamics and neuropathological development. In this study, we applied NIRCat to elucidate DA release in human induced pluripotent stem cells (hiPSCs)-derived dopaminergic neurons from both healthy control and PD patient carrying GBA1 mutations. We accurately quantified electrically stimulated DA release events, identifying distinct ‘hotspots’ of activity across DA neuronal cells. Our findings present a significantly enhanced spatial and temporal resolution of DA signaling, providing a deeper understanding of the role and balance of DA release in the progression of neurodegenerative disease. |
| format | Article |
| id | doaj-art-8bb54843c7454482a23a392a6808e80f |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-8bb54843c7454482a23a392a6808e80f2025-01-23T05:27:38ZengElsevierMaterials Today Bio2590-00642025-04-0131101485Nanosensor-based imaging of realtime dopamine release in neurons derived from iPSCs of patients with Parkinson's diseaseNayeon Lee0Dakyeon Lee1Jae Hyeok Lee2Bo Seok Lee3Sungjee Kim4Jae Ho Kim5Sanghwa Jeong6Convergence Stem Cell Research Center, Medical Research Institute, Pusan National University, Yangsan, 50612, Gyeongsangnam-do, Republic of Korea; Department of Physiology, Pusan National University School of Medicine, Yangsan, 50612, Gyeongsangnam-do, Republic of KoreaSchool of Biomedical Convergence Engineering, Pusan National University, Yangsan, 50612, Gyeongsangnam-do, Republic of Korea; Department of Chemistry, Pohang University of Science and Technology, Pohang, 37673, Republic of KoreaDepartment of Neurology, School of Medicine, Pusan National University Yangsan Hospital, Yangsan, 50612, Gyeongsangnam-do, Republic of KoreaConvergence Stem Cell Research Center, Medical Research Institute, Pusan National University, Yangsan, 50612, Gyeongsangnam-do, Republic of Korea; Department of Physiology, Pusan National University School of Medicine, Yangsan, 50612, Gyeongsangnam-do, Republic of KoreaDepartment of Chemistry, Pohang University of Science and Technology, Pohang, 37673, Republic of KoreaConvergence Stem Cell Research Center, Medical Research Institute, Pusan National University, Yangsan, 50612, Gyeongsangnam-do, Republic of Korea; Department of Physiology, Pusan National University School of Medicine, Yangsan, 50612, Gyeongsangnam-do, Republic of Korea; Corresponding author. Department of Physiology, Pusan National University School of Medicine, Yangsan, 50612, Gyeongsangnam-do, Republic of Korea.School of Biomedical Convergence Engineering, Pusan National University, Yangsan, 50612, Gyeongsangnam-do, Republic of Korea; Corresponding author. Department of Biomedical Convergence Engineering, Pusan National University, Yangsan, 50612, Gyeongsangnam-do, Republic of Korea.Dopamine (DA) is an essential neuromodulator that underlies critical aspects of cognitive processes, motor function, and reward systems. Disruptions in DA signaling contribute to various neurodegenerative diseases, including Parkinson's disease (PD). Despite its important role in neuronal function, the impact of DA release/uptake on neurochemical imbalances during neuronal development remains unclear. We propose a novel application of near-infrared catecholamine nanosensor (NIRCat) for real-time visualization of DA neurotransmission among neurodegenerative disease cells. The near-infrared fluorescence (900–1400 nm) of NIRCat allows the semi-quantitative measurement of DA release in living neurons and offers insights into cellular dynamics and neuropathological development. In this study, we applied NIRCat to elucidate DA release in human induced pluripotent stem cells (hiPSCs)-derived dopaminergic neurons from both healthy control and PD patient carrying GBA1 mutations. We accurately quantified electrically stimulated DA release events, identifying distinct ‘hotspots’ of activity across DA neuronal cells. Our findings present a significantly enhanced spatial and temporal resolution of DA signaling, providing a deeper understanding of the role and balance of DA release in the progression of neurodegenerative disease.http://www.sciencedirect.com/science/article/pii/S2590006425000432Near-infrared fluorescenceDopamine releaseCarbon nanotubeReal-time visualizationParkinson's disease |
| spellingShingle | Nayeon Lee Dakyeon Lee Jae Hyeok Lee Bo Seok Lee Sungjee Kim Jae Ho Kim Sanghwa Jeong Nanosensor-based imaging of realtime dopamine release in neurons derived from iPSCs of patients with Parkinson's disease Materials Today Bio Near-infrared fluorescence Dopamine release Carbon nanotube Real-time visualization Parkinson's disease |
| title | Nanosensor-based imaging of realtime dopamine release in neurons derived from iPSCs of patients with Parkinson's disease |
| title_full | Nanosensor-based imaging of realtime dopamine release in neurons derived from iPSCs of patients with Parkinson's disease |
| title_fullStr | Nanosensor-based imaging of realtime dopamine release in neurons derived from iPSCs of patients with Parkinson's disease |
| title_full_unstemmed | Nanosensor-based imaging of realtime dopamine release in neurons derived from iPSCs of patients with Parkinson's disease |
| title_short | Nanosensor-based imaging of realtime dopamine release in neurons derived from iPSCs of patients with Parkinson's disease |
| title_sort | nanosensor based imaging of realtime dopamine release in neurons derived from ipscs of patients with parkinson s disease |
| topic | Near-infrared fluorescence Dopamine release Carbon nanotube Real-time visualization Parkinson's disease |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425000432 |
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