Investigation of potential Shiga toxin producing Escherichia coli (STEC) associated with a local foodborne outbreak using multidisciplinary approaches
Shiga toxin producing Escherichia coli (STEC) outbreak is a public health concern as it can potentially cause a variety of clinical manifestations including diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). However E. coli are generally innocuous commensal organisms, and there is a...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2016-09-01
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| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345301630091X |
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| Summary: | Shiga toxin producing Escherichia coli (STEC) outbreak is a public health concern as it can potentially cause a variety of clinical manifestations including diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). However E. coli are generally innocuous commensal organisms, and there is a need to discriminate pathogenic from non-pathogenic isolates rapidly and accurately. In this study, we have used standard culture based methods and advanced molecular approaches to characterize E. coli in food in a local outbreak investigation. We show that the application of DNA based detection methods including real-time PCR and DNA microarray along with a traditional culture method can identify the organism implicated in an outbreak at the strain level for pathogenic potential. |
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| ISSN: | 2213-4530 |