Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves
Transgene introgression is a major concern associated with transgenic plant-based vaccines. Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression. Here, we introduce a new vaccine modality in which Staphylococcal enterotoxin B (SEB) genes are agroinfilt...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/3710961 |
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| author | Pei-Feng Liu Yanhan Wang Robert G. Ulrich Christopher W. Simmons Jean S. VanderGheynst Richard L. Gallo Chun-Ming Huang |
| author_facet | Pei-Feng Liu Yanhan Wang Robert G. Ulrich Christopher W. Simmons Jean S. VanderGheynst Richard L. Gallo Chun-Ming Huang |
| author_sort | Pei-Feng Liu |
| collection | DOAJ |
| description | Transgene introgression is a major concern associated with transgenic plant-based vaccines. Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression. Here, we introduce a new vaccine modality in which Staphylococcal enterotoxin B (SEB) genes are agroinfiltrated into radishes (Raphanw sativus L.), resulting in transient expression and accumulation of SEB in planta. This approach can simultaneously express multiple antigens in a single leaf. Furthermore, the potential of high-throughput vaccine production was demonstrated by simultaneously agroinfiltrating multiple radish leaves using a multichannel pipette. The expression of SEB was detectable in two leaf cell types (epidermal and guard cells) in agroinfiltrated leaves. ICR mice intranasally immunized with homogenized leaves agroinfiltrated with SEB elicited detectable antibody to SEB and displayed protection against SEB-induced interferon-gamma (IFN-γ) production. The concept of encapsulating antigens in leaves rather than purifying them for immunization may facilitate rapid vaccine production during an epidemic disease. |
| format | Article |
| id | doaj-art-8b7d16b670fa4557bb875460ad610bb2 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-8b7d16b670fa4557bb875460ad610bb22025-02-03T05:52:09ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/37109613710961Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish LeavesPei-Feng Liu0Yanhan Wang1Robert G. Ulrich2Christopher W. Simmons3Jean S. VanderGheynst4Richard L. Gallo5Chun-Ming Huang6Department of Dermatology, University of California, San Diego, CA 92161, USADepartment of Dermatology, University of California, San Diego, CA 92161, USADepartment of Immunology, Army Medical Research Institute of Infectious Diseases, Frederick, MD 21703, USADepartment of Biological & Agricultural Engineering, University of California, Davis, CA 95616, USADepartment of Biological & Agricultural Engineering, University of California, Davis, CA 95616, USADepartment of Dermatology, University of California, San Diego, CA 92161, USADepartment of Dermatology, University of California, San Diego, CA 92161, USATransgene introgression is a major concern associated with transgenic plant-based vaccines. Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression. Here, we introduce a new vaccine modality in which Staphylococcal enterotoxin B (SEB) genes are agroinfiltrated into radishes (Raphanw sativus L.), resulting in transient expression and accumulation of SEB in planta. This approach can simultaneously express multiple antigens in a single leaf. Furthermore, the potential of high-throughput vaccine production was demonstrated by simultaneously agroinfiltrating multiple radish leaves using a multichannel pipette. The expression of SEB was detectable in two leaf cell types (epidermal and guard cells) in agroinfiltrated leaves. ICR mice intranasally immunized with homogenized leaves agroinfiltrated with SEB elicited detectable antibody to SEB and displayed protection against SEB-induced interferon-gamma (IFN-γ) production. The concept of encapsulating antigens in leaves rather than purifying them for immunization may facilitate rapid vaccine production during an epidemic disease.http://dx.doi.org/10.1155/2018/3710961 |
| spellingShingle | Pei-Feng Liu Yanhan Wang Robert G. Ulrich Christopher W. Simmons Jean S. VanderGheynst Richard L. Gallo Chun-Ming Huang Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves Journal of Immunology Research |
| title | Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves |
| title_full | Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves |
| title_fullStr | Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves |
| title_full_unstemmed | Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves |
| title_short | Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves |
| title_sort | leaf encapsulated vaccines agroinfiltration and transient expression of the antigen staphylococcal endotoxin b in radish leaves |
| url | http://dx.doi.org/10.1155/2018/3710961 |
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