Acalypha Wilkesiana ‘Java White’: Identification of Some Bioactive Compounds by Gc-Ms and Their Effects on Key Enzymes Linked to Type 2 Diabete
In this study, we identified bioactive compounds from the ethanolic extracts of the leaves, stem bark and root bark of Acalypha wilkesiana through GC-MS analysis and investigated the effects of these extracts on some of the enzymes linked to type 2 diabetes. Plant parts were extracted sequentially w...
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2018-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2018-0037 |
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| author | Oyebode Olajumoke A. Erukainure Ochuko L. Koorbanally Neil A. Islam Md. Shahidul |
| author_facet | Oyebode Olajumoke A. Erukainure Ochuko L. Koorbanally Neil A. Islam Md. Shahidul |
| author_sort | Oyebode Olajumoke A. |
| collection | DOAJ |
| description | In this study, we identified bioactive compounds from the ethanolic extracts of the leaves, stem bark and root bark of Acalypha wilkesiana through GC-MS analysis and investigated the effects of these extracts on some of the enzymes linked to type 2 diabetes. Plant parts were extracted sequentially with ethyl acetate, ethanol and water. GC-MS analysis revealed the presence of long-chain alkyl acids, esters, ketones and alcohols including phytol and phytol acetate along with some secondary metabolites such as xanthone, vitamin E and various types of sterols including stigmasterol, campesterol and sitosterol. Ethanolic extracts of all the parts showed a dose- -dependent inhibition of α-glucosidase and α-amylase activity. The extracts also demonstrated anti-lipase activity. The ethanolic extract of root bark showed the highest inhibition of enzymes compared to other extracts. The EC50 values (concentrations for 50 % inhibition) of α-glucosidase, α-amylase and lipase inhibition were 35.75 ± 1.95, 6.25 ± 1.05 and 101.33 ± 5.21 μg mL-1, resp. The study suggests that A. wilkesiana ethanolic extracts have the ability to inhibit the activity of enzymes linked to type 2 diabetes. Further studies are needed to confirm the responsible bioactive compounds in this regard. |
| format | Article |
| id | doaj-art-8b5689a88f784eea80dc1a0e1b5e3212 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-8b5689a88f784eea80dc1a0e1b5e32122025-02-02T17:46:44ZengSciendoActa Pharmaceutica1846-95582018-12-0168442543910.2478/acph-2018-0037acph-2018-0037Acalypha Wilkesiana ‘Java White’: Identification of Some Bioactive Compounds by Gc-Ms and Their Effects on Key Enzymes Linked to Type 2 DiabeteOyebode Olajumoke A.0Erukainure Ochuko L.1Koorbanally Neil A.2Islam Md. Shahidul3Department of Biochemistry, School of Life Sciences, University of KwaZulu- Natal (Westville Campus), Durban 4000,Durban, South AfricaDepartment of Biochemistry, School of Life Sciences, University of KwaZulu- Natal (Westville Campus), Durban 4000,Durban, South AfricaSchool of Chemistry and Physics University of KwaZulu-Natal (Westville Campus), Durban 4000,Durban, South AfricaDepartment of Biochemistry, School of Life Sciences, University of KwaZulu- Natal (Westville Campus), Durban 4000,Durban, South AfricaIn this study, we identified bioactive compounds from the ethanolic extracts of the leaves, stem bark and root bark of Acalypha wilkesiana through GC-MS analysis and investigated the effects of these extracts on some of the enzymes linked to type 2 diabetes. Plant parts were extracted sequentially with ethyl acetate, ethanol and water. GC-MS analysis revealed the presence of long-chain alkyl acids, esters, ketones and alcohols including phytol and phytol acetate along with some secondary metabolites such as xanthone, vitamin E and various types of sterols including stigmasterol, campesterol and sitosterol. Ethanolic extracts of all the parts showed a dose- -dependent inhibition of α-glucosidase and α-amylase activity. The extracts also demonstrated anti-lipase activity. The ethanolic extract of root bark showed the highest inhibition of enzymes compared to other extracts. The EC50 values (concentrations for 50 % inhibition) of α-glucosidase, α-amylase and lipase inhibition were 35.75 ± 1.95, 6.25 ± 1.05 and 101.33 ± 5.21 μg mL-1, resp. The study suggests that A. wilkesiana ethanolic extracts have the ability to inhibit the activity of enzymes linked to type 2 diabetes. Further studies are needed to confirm the responsible bioactive compounds in this regard.https://doi.org/10.2478/acph-2018-0037acalypha wilkesianaα-glucosidaseα-amylaselipasetype 2 diabetesethanolic extract |
| spellingShingle | Oyebode Olajumoke A. Erukainure Ochuko L. Koorbanally Neil A. Islam Md. Shahidul Acalypha Wilkesiana ‘Java White’: Identification of Some Bioactive Compounds by Gc-Ms and Their Effects on Key Enzymes Linked to Type 2 Diabete Acta Pharmaceutica acalypha wilkesiana α-glucosidase α-amylase lipase type 2 diabetes ethanolic extract |
| title | Acalypha Wilkesiana ‘Java White’: Identification of Some Bioactive Compounds by Gc-Ms and Their Effects on Key Enzymes Linked to Type 2 Diabete |
| title_full | Acalypha Wilkesiana ‘Java White’: Identification of Some Bioactive Compounds by Gc-Ms and Their Effects on Key Enzymes Linked to Type 2 Diabete |
| title_fullStr | Acalypha Wilkesiana ‘Java White’: Identification of Some Bioactive Compounds by Gc-Ms and Their Effects on Key Enzymes Linked to Type 2 Diabete |
| title_full_unstemmed | Acalypha Wilkesiana ‘Java White’: Identification of Some Bioactive Compounds by Gc-Ms and Their Effects on Key Enzymes Linked to Type 2 Diabete |
| title_short | Acalypha Wilkesiana ‘Java White’: Identification of Some Bioactive Compounds by Gc-Ms and Their Effects on Key Enzymes Linked to Type 2 Diabete |
| title_sort | acalypha wilkesiana java white identification of some bioactive compounds by gc ms and their effects on key enzymes linked to type 2 diabete |
| topic | acalypha wilkesiana α-glucosidase α-amylase lipase type 2 diabetes ethanolic extract |
| url | https://doi.org/10.2478/acph-2018-0037 |
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