Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes
Bone Morphogenetic Protein (BMP-4) was shown to down-regulate melanogenesis, in part, by decreasing the level of tyrosinase [Yaar et al. (2006) JBC:281]. Results presented here show that BMP-4 down-regulated the protein levels of TRP-1, PKC-β, and MCI-R. When paired cultures of human melanocytes wer...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2009/750482 |
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| author | Hee-Young Park Christina Wu Mina Yaar Christina M. Stachur Marita Kosmadaki Barbara A. Gilchrest |
| author_facet | Hee-Young Park Christina Wu Mina Yaar Christina M. Stachur Marita Kosmadaki Barbara A. Gilchrest |
| author_sort | Hee-Young Park |
| collection | DOAJ |
| description | Bone Morphogenetic Protein (BMP-4) was shown to down-regulate melanogenesis, in part, by decreasing the level of tyrosinase [Yaar et al. (2006) JBC:281]. Results presented here show that BMP-4 down-regulated the protein levels of TRP-1, PKC-β, and MCI-R. When paired cultures of human melanocytes were treated with vehicle or BMP-4 (25 ng/ml), MAPK/ERK were phosphorylated within one hour of BMP-4 treatment. Then the activated MAPK/ERK caused an acute phosphorylation of MITF, followed by proteosome-mediated degradation of MITF, the key transcription factor for melanogenic proteins [Wu et al. (2000) Gene & Development:14]. However, prolonged exposure of melanocytes to BMP-4 (up to 48 hours) caused a decrease in the level of MITF-M transcript. In addition, BMP-4 decreased the intracellular level of cAMP, the key regulator of MITF expression. These results demonstrate that BMP-4 activates MAPK/ERK signaling pathway to transiently activate MITF; however, chronic treatment of BMP-4 to melanocytes causes a down-regulation of the expression of MITF, possibly in a cAMP-dependent pathway. |
| format | Article |
| id | doaj-art-8b4f1aaf8b754c73b1ff1cdef4ea6b45 |
| institution | Kabale University |
| issn | 1687-8876 1687-8884 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Cell Biology |
| spelling | doaj-art-8b4f1aaf8b754c73b1ff1cdef4ea6b452025-02-03T01:27:11ZengWileyInternational Journal of Cell Biology1687-88761687-88842009-01-01200910.1155/2009/750482750482Role of BMP-4 and Its Signaling Pathways in Cultured Human MelanocytesHee-Young Park0Christina Wu1Mina Yaar2Christina M. Stachur3Marita Kosmadaki4Barbara A. Gilchrest5Department of Dermatology, School of Medicine, Boston University, Boston, MA 02118, USADepartment of Dermatology, School of Medicine, Boston University, Boston, MA 02118, USADepartment of Dermatology, School of Medicine, Boston University, Boston, MA 02118, USADepartment of Dermatology, School of Medicine, Boston University, Boston, MA 02118, USADepartment of Dermatology, School of Medicine, Boston University, Boston, MA 02118, USADepartment of Dermatology, School of Medicine, Boston University, Boston, MA 02118, USABone Morphogenetic Protein (BMP-4) was shown to down-regulate melanogenesis, in part, by decreasing the level of tyrosinase [Yaar et al. (2006) JBC:281]. Results presented here show that BMP-4 down-regulated the protein levels of TRP-1, PKC-β, and MCI-R. When paired cultures of human melanocytes were treated with vehicle or BMP-4 (25 ng/ml), MAPK/ERK were phosphorylated within one hour of BMP-4 treatment. Then the activated MAPK/ERK caused an acute phosphorylation of MITF, followed by proteosome-mediated degradation of MITF, the key transcription factor for melanogenic proteins [Wu et al. (2000) Gene & Development:14]. However, prolonged exposure of melanocytes to BMP-4 (up to 48 hours) caused a decrease in the level of MITF-M transcript. In addition, BMP-4 decreased the intracellular level of cAMP, the key regulator of MITF expression. These results demonstrate that BMP-4 activates MAPK/ERK signaling pathway to transiently activate MITF; however, chronic treatment of BMP-4 to melanocytes causes a down-regulation of the expression of MITF, possibly in a cAMP-dependent pathway.http://dx.doi.org/10.1155/2009/750482 |
| spellingShingle | Hee-Young Park Christina Wu Mina Yaar Christina M. Stachur Marita Kosmadaki Barbara A. Gilchrest Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes International Journal of Cell Biology |
| title | Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes |
| title_full | Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes |
| title_fullStr | Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes |
| title_full_unstemmed | Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes |
| title_short | Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes |
| title_sort | role of bmp 4 and its signaling pathways in cultured human melanocytes |
| url | http://dx.doi.org/10.1155/2009/750482 |
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