Impact of Likelihood Ratios of Rheumatoid Factor and Anti-Cyclic Citrullinated Peptide Antibody in Clinical Diagnosis of Rheumatoid Arthritis by Two Available Platforms
<b>Background/Objectives</b>: Rheumatoid arthritis (RA) is one of the most prevalent autoimmune diseases, characterized by an articular and extra-articular involvement, where autoantibodies, such as rheumatoid factor (RF) and anti-cyclic citrullinated peptide antibodies (ACPAs), are impo...
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2025-01-01
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| author | Juan Irure-Ventura María Díaz-Toledo Noelia Palazuelos-Cayón Marcos López-Hoyos |
| author_facet | Juan Irure-Ventura María Díaz-Toledo Noelia Palazuelos-Cayón Marcos López-Hoyos |
| author_sort | Juan Irure-Ventura |
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| description | <b>Background/Objectives</b>: Rheumatoid arthritis (RA) is one of the most prevalent autoimmune diseases, characterized by an articular and extra-articular involvement, where autoantibodies, such as rheumatoid factor (RF) and anti-cyclic citrullinated peptide antibodies (ACPAs), are important biomarkers for the diagnosis. Autoantibody determination can be carried out using different assays. However, the results obtained are usually expressed in arbitrary units that are not comparable. Therefore, the aim of this study is to improve clinical interpretation of RF and ACPA test results using the likelihood ratio (LR). <b>Methods</b>: RF and ACPA titers were analyzed by turbidimetry and chemiluminescence using Optilite and BIO-FLASH systems, respectively, in 781 samples from patients with RA and in 1970 controls. <b>Results</b>: The higher the antibody titer of RF or ACPA, the higher the LR for RA. The definition of test result interval-specific LR based on predefined specificities for antibody levels provides more information than the use of the cut-off set by the manufacturer for each antibody. <b>Conclusions</b>: The LR for RA increased with an increasing antibody level. In addition, the use of test result interval-specific LR allows better clinical interpretation for RF and ACPA assays compared to the traditional idea of interpreting antibody results in a dichotomous manner, such as negative or positive. |
| format | Article |
| id | doaj-art-8b4cc47458ef458c99444fbf5bbf4e84 |
| institution | Kabale University |
| issn | 2075-4418 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Diagnostics |
| spelling | doaj-art-8b4cc47458ef458c99444fbf5bbf4e842025-01-24T13:28:51ZengMDPI AGDiagnostics2075-44182025-01-0115213510.3390/diagnostics15020135Impact of Likelihood Ratios of Rheumatoid Factor and Anti-Cyclic Citrullinated Peptide Antibody in Clinical Diagnosis of Rheumatoid Arthritis by Two Available PlatformsJuan Irure-Ventura0María Díaz-Toledo1Noelia Palazuelos-Cayón2Marcos López-Hoyos3Immunology Department, University Hospital Marqués de Valdecilla, 39008 Santander, SpainFaculty of Medicine, University of Cantabria, 39011 Santander, SpainFaculty of Medicine, University of Cantabria, 39011 Santander, SpainImmunology Department, University Hospital Marqués de Valdecilla, 39008 Santander, Spain<b>Background/Objectives</b>: Rheumatoid arthritis (RA) is one of the most prevalent autoimmune diseases, characterized by an articular and extra-articular involvement, where autoantibodies, such as rheumatoid factor (RF) and anti-cyclic citrullinated peptide antibodies (ACPAs), are important biomarkers for the diagnosis. Autoantibody determination can be carried out using different assays. However, the results obtained are usually expressed in arbitrary units that are not comparable. Therefore, the aim of this study is to improve clinical interpretation of RF and ACPA test results using the likelihood ratio (LR). <b>Methods</b>: RF and ACPA titers were analyzed by turbidimetry and chemiluminescence using Optilite and BIO-FLASH systems, respectively, in 781 samples from patients with RA and in 1970 controls. <b>Results</b>: The higher the antibody titer of RF or ACPA, the higher the LR for RA. The definition of test result interval-specific LR based on predefined specificities for antibody levels provides more information than the use of the cut-off set by the manufacturer for each antibody. <b>Conclusions</b>: The LR for RA increased with an increasing antibody level. In addition, the use of test result interval-specific LR allows better clinical interpretation for RF and ACPA assays compared to the traditional idea of interpreting antibody results in a dichotomous manner, such as negative or positive.https://www.mdpi.com/2075-4418/15/2/135rheumatoid arthritisrheumatoid factoranti-cyclic citrullinated peptide antibodieslikelihood ratio |
| spellingShingle | Juan Irure-Ventura María Díaz-Toledo Noelia Palazuelos-Cayón Marcos López-Hoyos Impact of Likelihood Ratios of Rheumatoid Factor and Anti-Cyclic Citrullinated Peptide Antibody in Clinical Diagnosis of Rheumatoid Arthritis by Two Available Platforms Diagnostics rheumatoid arthritis rheumatoid factor anti-cyclic citrullinated peptide antibodies likelihood ratio |
| title | Impact of Likelihood Ratios of Rheumatoid Factor and Anti-Cyclic Citrullinated Peptide Antibody in Clinical Diagnosis of Rheumatoid Arthritis by Two Available Platforms |
| title_full | Impact of Likelihood Ratios of Rheumatoid Factor and Anti-Cyclic Citrullinated Peptide Antibody in Clinical Diagnosis of Rheumatoid Arthritis by Two Available Platforms |
| title_fullStr | Impact of Likelihood Ratios of Rheumatoid Factor and Anti-Cyclic Citrullinated Peptide Antibody in Clinical Diagnosis of Rheumatoid Arthritis by Two Available Platforms |
| title_full_unstemmed | Impact of Likelihood Ratios of Rheumatoid Factor and Anti-Cyclic Citrullinated Peptide Antibody in Clinical Diagnosis of Rheumatoid Arthritis by Two Available Platforms |
| title_short | Impact of Likelihood Ratios of Rheumatoid Factor and Anti-Cyclic Citrullinated Peptide Antibody in Clinical Diagnosis of Rheumatoid Arthritis by Two Available Platforms |
| title_sort | impact of likelihood ratios of rheumatoid factor and anti cyclic citrullinated peptide antibody in clinical diagnosis of rheumatoid arthritis by two available platforms |
| topic | rheumatoid arthritis rheumatoid factor anti-cyclic citrullinated peptide antibodies likelihood ratio |
| url | https://www.mdpi.com/2075-4418/15/2/135 |
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