Sympatric non-biting flies serve as potential vectors of zoonotic protozoan parasites on pig farms in China

Abstract Background Cryptosporidium spp., Enterocytozoon bieneusi, and Blastocystis sp. are common enteric parasites in humans and pigs. Ascertaining whether non-biting flies (NBFs) serve as potential vectors of these parasites on pig farms is a crucial aspect of disease control. Methods Non-biting...

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Main Authors: Yufeng Liu, Pitambar Dhakal, Wenyan Hou, Fa Shan, Nanhao Wang, Bin Yang, Huikai Qin, Xiaoying Li, Rongjun Wang, Longxian Zhang, Sumei Zhang, Junqiang Li
Format: Article
Language:English
Published: BMC 2025-02-01
Series:Parasites & Vectors
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Online Access:https://doi.org/10.1186/s13071-025-06686-2
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author Yufeng Liu
Pitambar Dhakal
Wenyan Hou
Fa Shan
Nanhao Wang
Bin Yang
Huikai Qin
Xiaoying Li
Rongjun Wang
Longxian Zhang
Sumei Zhang
Junqiang Li
author_facet Yufeng Liu
Pitambar Dhakal
Wenyan Hou
Fa Shan
Nanhao Wang
Bin Yang
Huikai Qin
Xiaoying Li
Rongjun Wang
Longxian Zhang
Sumei Zhang
Junqiang Li
author_sort Yufeng Liu
collection DOAJ
description Abstract Background Cryptosporidium spp., Enterocytozoon bieneusi, and Blastocystis sp. are common enteric parasites in humans and pigs. Ascertaining whether non-biting flies (NBFs) serve as potential vectors of these parasites on pig farms is a crucial aspect of disease control. Methods Non-biting flies were collected and identified by morphology analysis together with sequence analysis of the mitochondrial cytochrome c oxidase I (CO1) gene as confirmation. In a cross-sectional study, the small-subunit ribosomal RNA (SSU rRNA) gene of Cryptosporidium spp., the internal transcribed spacer region (ITS) region of E. bieneusi and the SSU rRNA gene of Blastocystis sp. were investigated in fresh pig fecal samples and sympatric NBFs. Results The results revealed the occurrence of five species of NBFs (Musca domestica, 91.2%; Lucilia sericata, 5.8%; Chrysomya megacephala, 1.7%; Aldrichina grahami, 0.6%; Helicophagella melanura, 0.6%) in the collected pig fecal samples. The prevalence of Cryptosporidium spp., E. bieneusi and Blastocystis sp. on the body surface of NBFs was 0.6% (2/342), 4.4% (15/342) and 20.8% (71/342), respectively. Similarly, the prevalence of these parasites in the lysates of NBFs (= in vivo carriage) was 0% (0/342), 2.7% (9/342) and 10.5% (36/342), respectively. The prevalence of Cryptosporidium spp., E. bieneusi and Blastocystis sp. in pigs from which fly samples were collected was 2.3% (41/1794), 12.6% (226/1794) and 30.8% (553/1794), respectively. The zoonotic Cryposporidium suis/C. scrofarum, E. bieneusi ITS genotypes EbpA/EbpC and Blastocystis sp. subtypes ST1/ST3/ST5 were identified in both NBFs and pig feces. NBFs were found to carry E. bieneusi and Blastocystis sp. on their body surface as well as in the lysates. Conclusions These findings demonstrate the role of NBFs as potential vectors in the dissemination of these zoonotic parasites in pig farms, and also highlight the possibility of their transmission to humans. Graphical Abstract
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spelling doaj-art-8b322d5e35c84d6ea55a1ee6b075b3d02025-08-20T03:10:50ZengBMCParasites & Vectors1756-33052025-02-0118111210.1186/s13071-025-06686-2Sympatric non-biting flies serve as potential vectors of zoonotic protozoan parasites on pig farms in ChinaYufeng Liu0Pitambar Dhakal1Wenyan Hou2Fa Shan3Nanhao Wang4Bin Yang5Huikai Qin6Xiaoying Li7Rongjun Wang8Longxian Zhang9Sumei Zhang10Junqiang Li11College of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityRural Industry Development Center of Shangqiu CityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityAbstract Background Cryptosporidium spp., Enterocytozoon bieneusi, and Blastocystis sp. are common enteric parasites in humans and pigs. Ascertaining whether non-biting flies (NBFs) serve as potential vectors of these parasites on pig farms is a crucial aspect of disease control. Methods Non-biting flies were collected and identified by morphology analysis together with sequence analysis of the mitochondrial cytochrome c oxidase I (CO1) gene as confirmation. In a cross-sectional study, the small-subunit ribosomal RNA (SSU rRNA) gene of Cryptosporidium spp., the internal transcribed spacer region (ITS) region of E. bieneusi and the SSU rRNA gene of Blastocystis sp. were investigated in fresh pig fecal samples and sympatric NBFs. Results The results revealed the occurrence of five species of NBFs (Musca domestica, 91.2%; Lucilia sericata, 5.8%; Chrysomya megacephala, 1.7%; Aldrichina grahami, 0.6%; Helicophagella melanura, 0.6%) in the collected pig fecal samples. The prevalence of Cryptosporidium spp., E. bieneusi and Blastocystis sp. on the body surface of NBFs was 0.6% (2/342), 4.4% (15/342) and 20.8% (71/342), respectively. Similarly, the prevalence of these parasites in the lysates of NBFs (= in vivo carriage) was 0% (0/342), 2.7% (9/342) and 10.5% (36/342), respectively. The prevalence of Cryptosporidium spp., E. bieneusi and Blastocystis sp. in pigs from which fly samples were collected was 2.3% (41/1794), 12.6% (226/1794) and 30.8% (553/1794), respectively. The zoonotic Cryposporidium suis/C. scrofarum, E. bieneusi ITS genotypes EbpA/EbpC and Blastocystis sp. subtypes ST1/ST3/ST5 were identified in both NBFs and pig feces. NBFs were found to carry E. bieneusi and Blastocystis sp. on their body surface as well as in the lysates. Conclusions These findings demonstrate the role of NBFs as potential vectors in the dissemination of these zoonotic parasites in pig farms, and also highlight the possibility of their transmission to humans. Graphical Abstracthttps://doi.org/10.1186/s13071-025-06686-2Non-biting fliesPig farmsCryptosporidium spp.Enterocytozoon bieneusiBlastocystis sp.
spellingShingle Yufeng Liu
Pitambar Dhakal
Wenyan Hou
Fa Shan
Nanhao Wang
Bin Yang
Huikai Qin
Xiaoying Li
Rongjun Wang
Longxian Zhang
Sumei Zhang
Junqiang Li
Sympatric non-biting flies serve as potential vectors of zoonotic protozoan parasites on pig farms in China
Parasites & Vectors
Non-biting flies
Pig farms
Cryptosporidium spp.
Enterocytozoon bieneusi
Blastocystis sp.
title Sympatric non-biting flies serve as potential vectors of zoonotic protozoan parasites on pig farms in China
title_full Sympatric non-biting flies serve as potential vectors of zoonotic protozoan parasites on pig farms in China
title_fullStr Sympatric non-biting flies serve as potential vectors of zoonotic protozoan parasites on pig farms in China
title_full_unstemmed Sympatric non-biting flies serve as potential vectors of zoonotic protozoan parasites on pig farms in China
title_short Sympatric non-biting flies serve as potential vectors of zoonotic protozoan parasites on pig farms in China
title_sort sympatric non biting flies serve as potential vectors of zoonotic protozoan parasites on pig farms in china
topic Non-biting flies
Pig farms
Cryptosporidium spp.
Enterocytozoon bieneusi
Blastocystis sp.
url https://doi.org/10.1186/s13071-025-06686-2
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