Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells
Human Vγ9Vδ2 T cells recognise pyrophosphate-based antigens (phosphoantigens) and have multiple functions in innate and adaptive immunity, including a unique ability to activate other cells of the immune system. We used flow cytometry and ELISA to define the early cytokine profiles of Vγ9Vδ2 T cel...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2010/704941 |
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| author | Margaret R. Dunne Bozgana A. Mangan Laura Madrigal-Estebas Derek G. Doherty |
| author_facet | Margaret R. Dunne Bozgana A. Mangan Laura Madrigal-Estebas Derek G. Doherty |
| author_sort | Margaret R. Dunne |
| collection | DOAJ |
| description | Human Vγ9Vδ2 T cells recognise pyrophosphate-based antigens (phosphoantigens) and have multiple functions in innate and adaptive immunity, including a unique ability to activate other cells of the immune system. We used flow cytometry and ELISA to define the early cytokine profiles of Vγ9Vδ2 T cells stimulated in vitro with isopentenyl pyrophosphate (IPP) and (E)-4-hydroxy-3-methyl-but-2 enyl pyrophosphate (HMB-PP) in the absence and presence of IL-2 and IL-15. We show that fresh Vγ9Vδ2 T cells produce interferon-γ (IFN-γ) and tumour necrosis factor-α (TNF-α) within 4 hours of stimulation with phosphoantigen, but neither IL-10, IL-13, nor IL-17 was detectable up to 72 hours under these
conditions. Cytokine production was not influenced by expression or lack, thereof, of CD4 or CD8. Addition of IL-2 or IL-15 caused expansion of IFN-γ-producing Vγ9Vδ2 T cells, but did not enhance IFN-γ secretion after 24–72 hours. Thus, phosphoantigen-stimulated Vγ9Vδ2 T cells have potential as Th1-biasing adjuvants for immunotherapy. |
| format | Article |
| id | doaj-art-8b0fba9783884a6486c46f29b7fee891 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-8b0fba9783884a6486c46f29b7fee8912025-02-03T06:01:40ZengWileyMediators of Inflammation0962-93511466-18612010-01-01201010.1155/2010/704941704941Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T CellsMargaret R. Dunne0Bozgana A. Mangan1Laura Madrigal-Estebas2Derek G. Doherty3Department of Immunology and Institute of Molecular Medicine, Trinity College Dublin, St. James's Hospital, Dublin 8, IrelandDepartment of Immunology and Institute of Molecular Medicine, Trinity College Dublin, St. James's Hospital, Dublin 8, IrelandInstitute of Immunology, National University of Ireland, Maynooth, Co. Kildare, IrelandDepartment of Immunology and Institute of Molecular Medicine, Trinity College Dublin, St. James's Hospital, Dublin 8, IrelandHuman Vγ9Vδ2 T cells recognise pyrophosphate-based antigens (phosphoantigens) and have multiple functions in innate and adaptive immunity, including a unique ability to activate other cells of the immune system. We used flow cytometry and ELISA to define the early cytokine profiles of Vγ9Vδ2 T cells stimulated in vitro with isopentenyl pyrophosphate (IPP) and (E)-4-hydroxy-3-methyl-but-2 enyl pyrophosphate (HMB-PP) in the absence and presence of IL-2 and IL-15. We show that fresh Vγ9Vδ2 T cells produce interferon-γ (IFN-γ) and tumour necrosis factor-α (TNF-α) within 4 hours of stimulation with phosphoantigen, but neither IL-10, IL-13, nor IL-17 was detectable up to 72 hours under these conditions. Cytokine production was not influenced by expression or lack, thereof, of CD4 or CD8. Addition of IL-2 or IL-15 caused expansion of IFN-γ-producing Vγ9Vδ2 T cells, but did not enhance IFN-γ secretion after 24–72 hours. Thus, phosphoantigen-stimulated Vγ9Vδ2 T cells have potential as Th1-biasing adjuvants for immunotherapy.http://dx.doi.org/10.1155/2010/704941 |
| spellingShingle | Margaret R. Dunne Bozgana A. Mangan Laura Madrigal-Estebas Derek G. Doherty Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells Mediators of Inflammation |
| title | Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells |
| title_full | Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells |
| title_fullStr | Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells |
| title_full_unstemmed | Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells |
| title_short | Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells |
| title_sort | preferential th1 cytokine profile of phosphoantigen stimulated human vγ9vδ2 t cells |
| url | http://dx.doi.org/10.1155/2010/704941 |
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