In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion Model
A poly-70L/30DL-lactide (PLA70)–β-tricalcium phosphate (β-TCP) composite implant reinforced by continuous PLA-96L/4D-lactide (PLA96) fibers was designed for in vivo spinal fusion. The pilot study was performed with four sheep, using titanium cage implants as controls. The composite implants failed t...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2011/109638 |
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| author | Janek Frantzén Aliisa Pälli Esa Kotilainen Harri Heino Bettina Mannerström Heini Huhtala Hannu Kuokkanen George K. Sándor Kari Leino Matias Röyttä Riitta Parkkola Riitta Suuronen Susanna Miettinen Hannu T. Aro Suvi Haimi |
| author_facet | Janek Frantzén Aliisa Pälli Esa Kotilainen Harri Heino Bettina Mannerström Heini Huhtala Hannu Kuokkanen George K. Sándor Kari Leino Matias Röyttä Riitta Parkkola Riitta Suuronen Susanna Miettinen Hannu T. Aro Suvi Haimi |
| author_sort | Janek Frantzén |
| collection | DOAJ |
| description | A poly-70L/30DL-lactide (PLA70)–β-tricalcium phosphate (β-TCP) composite implant reinforced by continuous PLA-96L/4D-lactide (PLA96) fibers was designed for in vivo spinal fusion. The pilot study was performed with four sheep, using titanium cage implants as controls. The composite implants failed to direct bone growth as desired, whereas the bone contact and the proper integration were evident with controls 6 months after implantation. Therefore, the PLA70/β-TCP composite matrix material was further analyzed in the in vitro experiment by human and ovine adipose stem cells (hASCs and oASCs). The composites proved to be biocompatible as confirmed by live/dead assay. The proliferation rate of oASCs was higher than that of hASCs at all times during the 28 d culture period. Furthermore, the composites had only a minor osteogenic effect on oASCs, whereas the hASC osteogenesis on PLA70/β-TCP composites was evident. In conclusion, the composite implant material can be applied with hASCs for tissue engineering but not be evaluated in vivo with sheep. |
| format | Article |
| id | doaj-art-8aefe406b11c465e8e72493a5f0037d6 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-8aefe406b11c465e8e72493a5f0037d62025-02-03T06:48:28ZengWileyInternational Journal of Biomaterials1687-87871687-87952011-01-01201110.1155/2011/109638109638In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion ModelJanek Frantzén0Aliisa Pälli1Esa Kotilainen2Harri Heino3Bettina Mannerström4Heini Huhtala5Hannu Kuokkanen6George K. Sándor7Kari Leino8Matias Röyttä9Riitta Parkkola10Riitta Suuronen11Susanna Miettinen12Hannu T. Aro13Suvi Haimi14Neurosurgical Unit, Department of Surgery, Turku University Central Hospital, P.O. Box 52, 20521 Turku, FinlandInstitute of Biomedical Technology, University of Tampere, 33014 Tampere, FinlandNeurosurgical Unit, Department of Surgery, Turku University Central Hospital, P.O. Box 52, 20521 Turku, FinlandBioretec Ltd., Hermiankatu 22, 33720 Tampere, FinlandInstitute of Biomedical Technology, University of Tampere, 33014 Tampere, FinlandTampere School of Public Health, University of Tampere, 33014 Tampere, FinlandDepartment of Plastic Surgery, Tampere University Hospital, P.O. Box 2000, 33521 Tampere, FinlandInstitute of Biomedical Technology, University of Tampere, 33014 Tampere, FinlandDepartment of Anaesthesiology, Intensive Care, Emergency Care and Pain Medicine, Turku University Central Hospital, P.O. Box 52, 20521 Turku, FinlandDepartment of Pathology, Turku University Central Hospital, P.O. Box 52, 20521 Turku, FinlandDepartment of Radiology, Turku University Central Hospital, P.O. Box 52, 20521 Turku, FinlandInstitute of Biomedical Technology, University of Tampere, 33014 Tampere, FinlandInstitute of Biomedical Technology, University of Tampere, 33014 Tampere, FinlandOrthopedic Research Unit, Department of Orthopedic Surgery and Traumatology, University of Turku, Lemminkäisenkatu 2, 20520 Turku, FinlandInstitute of Biomedical Technology, University of Tampere, 33014 Tampere, FinlandA poly-70L/30DL-lactide (PLA70)–β-tricalcium phosphate (β-TCP) composite implant reinforced by continuous PLA-96L/4D-lactide (PLA96) fibers was designed for in vivo spinal fusion. The pilot study was performed with four sheep, using titanium cage implants as controls. The composite implants failed to direct bone growth as desired, whereas the bone contact and the proper integration were evident with controls 6 months after implantation. Therefore, the PLA70/β-TCP composite matrix material was further analyzed in the in vitro experiment by human and ovine adipose stem cells (hASCs and oASCs). The composites proved to be biocompatible as confirmed by live/dead assay. The proliferation rate of oASCs was higher than that of hASCs at all times during the 28 d culture period. Furthermore, the composites had only a minor osteogenic effect on oASCs, whereas the hASC osteogenesis on PLA70/β-TCP composites was evident. In conclusion, the composite implant material can be applied with hASCs for tissue engineering but not be evaluated in vivo with sheep.http://dx.doi.org/10.1155/2011/109638 |
| spellingShingle | Janek Frantzén Aliisa Pälli Esa Kotilainen Harri Heino Bettina Mannerström Heini Huhtala Hannu Kuokkanen George K. Sándor Kari Leino Matias Röyttä Riitta Parkkola Riitta Suuronen Susanna Miettinen Hannu T. Aro Suvi Haimi In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion Model International Journal of Biomaterials |
| title | In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion Model |
| title_full | In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion Model |
| title_fullStr | In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion Model |
| title_full_unstemmed | In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion Model |
| title_short | In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion Model |
| title_sort | in vivo and in vitro study of a polylactide fiber reinforced β tricalcium phosphate composite cage in an ovine anterior cervical intercorporal fusion model |
| url | http://dx.doi.org/10.1155/2011/109638 |
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