pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films
Bacteriorhodopsin (bR) multilayer films were successfully prepared by alternatingdepositions of cationic poly(diallyldimethylammonium chloride) (PDAC) polyelectrolyte and purple membrane (PM). The advantage of the method when compared to other thin film techniques is its simplicity to prepare large...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2000-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/S1110662X00000064 |
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| Summary: | Bacteriorhodopsin (bR) multilayer films were successfully prepared by alternatingdepositions
of cationic poly(diallyldimethylammonium chloride) (PDAC) polyelectrolyte and purple membrane (PM). The
advantage of the method when compared to other thin film techniques is its simplicity to prepare large selfassembled
films. The orientations of the bR molecules in the films were studied by a transient photovoltage
method. The polarity of the photoresponse signals showed that in basic conditions the cytoplasmic side of PM
was adsorbed onto the polyelectrolyte layer, whereas in acidic conditions the extracellular side was adsorbed
onto the polyelectrolyte layer. The photocycle of the bR in the films was studied by usingthe conventional
flash photolysis method. The photochemical behavior of bR in dry polycationic films was similar to that in
other types of dry films and the intermediate lifetimes were almost independent of the protein orientation. |
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| ISSN: | 1110-662X |