Effects of mti-2 Transgenic Potato Plants on the Aphid Myzus persicae (Sternorrhyncha: Aphididae)
Overexpressed in transgenic plants, protease inhibitors showed insecticidal effects against several insect taxa. We transformed potato internodes with the mustard trypsin inhibitor mti-2 gene. Among the 35 independent transgenic potato lines obtained via Agrobacterium tumefasciens transformation, fo...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Agronomy |
| Online Access: | http://dx.doi.org/10.1155/2010/653431 |
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| author | Julien Saguez Anas Cherqui Salima Lehraiki Charles Vincent Antony Beaujean Lise Jouanin Jean-Claude Laberche Philippe Giordanengo |
| author_facet | Julien Saguez Anas Cherqui Salima Lehraiki Charles Vincent Antony Beaujean Lise Jouanin Jean-Claude Laberche Philippe Giordanengo |
| author_sort | Julien Saguez |
| collection | DOAJ |
| description | Overexpressed in transgenic plants, protease inhibitors showed insecticidal effects against several insect taxa. We transformed potato internodes with the mustard trypsin inhibitor mti-2 gene. Among the 35 independent transgenic potato lines obtained via Agrobacterium tumefasciens transformation, four (DM6, DM7, DM11, and DM19) were selected for their high level of MTI-2 (at least to 30% of trypsin activity inhibition). Feeding assays were carried out to evaluate their effects on the green-peach aphid, Myzus persicae (Sternorrhyncha: Aphididae). Prereproductive period, nymphal mortality, adult fecundity, and doubling time of M. persicae populations were monitored on nontransformed potato plants (NT) and the four selected DM lines. Compared to NT plants, DM19 did not induce any effect on M. persicae. In contrast, DM7 and DM11 increased nymphal survival by approximately 20%. DM6 and DM11 lines slightly enhanced M. persicae daily fecundity and intrinsic rate of natural increase, leading to a reduction of the doubling time of the populations by 1 day. DM6 did not impact nymphal mortality, whereas with the DM11 almost all the nymphs survived. Potato plants transformed with the mti-2 gene variably affected the life history of M. persicae but did not show any insecticidal effect on the aphid. |
| format | Article |
| id | doaj-art-8ad01538972f44e1941a0daa783053e8 |
| institution | Kabale University |
| issn | 1687-8159 1687-8167 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Agronomy |
| spelling | doaj-art-8ad01538972f44e1941a0daa783053e82025-02-03T01:00:21ZengWileyInternational Journal of Agronomy1687-81591687-81672010-01-01201010.1155/2010/653431653431Effects of mti-2 Transgenic Potato Plants on the Aphid Myzus persicae (Sternorrhyncha: Aphididae)Julien Saguez0Anas Cherqui1Salima Lehraiki2Charles Vincent3Antony Beaujean4Lise Jouanin5Jean-Claude Laberche6Philippe Giordanengo7Biologie des Plantes et Contrôle des Insectes Ravageurs (UPRES EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, FranceBiologie des Plantes et Contrôle des Insectes Ravageurs (UPRES EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, FranceBiologie des Plantes et Contrôle des Insectes Ravageurs (UPRES EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, FranceCentre de Recherche et de Développement en Horticulture, Agriculture et Agro-Alimentaire Canada, 430 Boul. Gouin, Saint-Jean-sur-Richelieu, QC, J3B 3E6, CanadaBiologie des Plantes et Contrôle des Insectes Ravageurs (UPRES EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, FranceBiologie Cellulaire, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, FranceBiologie des Plantes et Contrôle des Insectes Ravageurs (UPRES EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, FranceBiologie des Plantes et Contrôle des Insectes Ravageurs (UPRES EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, FranceOverexpressed in transgenic plants, protease inhibitors showed insecticidal effects against several insect taxa. We transformed potato internodes with the mustard trypsin inhibitor mti-2 gene. Among the 35 independent transgenic potato lines obtained via Agrobacterium tumefasciens transformation, four (DM6, DM7, DM11, and DM19) were selected for their high level of MTI-2 (at least to 30% of trypsin activity inhibition). Feeding assays were carried out to evaluate their effects on the green-peach aphid, Myzus persicae (Sternorrhyncha: Aphididae). Prereproductive period, nymphal mortality, adult fecundity, and doubling time of M. persicae populations were monitored on nontransformed potato plants (NT) and the four selected DM lines. Compared to NT plants, DM19 did not induce any effect on M. persicae. In contrast, DM7 and DM11 increased nymphal survival by approximately 20%. DM6 and DM11 lines slightly enhanced M. persicae daily fecundity and intrinsic rate of natural increase, leading to a reduction of the doubling time of the populations by 1 day. DM6 did not impact nymphal mortality, whereas with the DM11 almost all the nymphs survived. Potato plants transformed with the mti-2 gene variably affected the life history of M. persicae but did not show any insecticidal effect on the aphid.http://dx.doi.org/10.1155/2010/653431 |
| spellingShingle | Julien Saguez Anas Cherqui Salima Lehraiki Charles Vincent Antony Beaujean Lise Jouanin Jean-Claude Laberche Philippe Giordanengo Effects of mti-2 Transgenic Potato Plants on the Aphid Myzus persicae (Sternorrhyncha: Aphididae) International Journal of Agronomy |
| title | Effects of mti-2 Transgenic Potato Plants on the Aphid Myzus persicae (Sternorrhyncha: Aphididae) |
| title_full | Effects of mti-2 Transgenic Potato Plants on the Aphid Myzus persicae (Sternorrhyncha: Aphididae) |
| title_fullStr | Effects of mti-2 Transgenic Potato Plants on the Aphid Myzus persicae (Sternorrhyncha: Aphididae) |
| title_full_unstemmed | Effects of mti-2 Transgenic Potato Plants on the Aphid Myzus persicae (Sternorrhyncha: Aphididae) |
| title_short | Effects of mti-2 Transgenic Potato Plants on the Aphid Myzus persicae (Sternorrhyncha: Aphididae) |
| title_sort | effects of mti 2 transgenic potato plants on the aphid myzus persicae sternorrhyncha aphididae |
| url | http://dx.doi.org/10.1155/2010/653431 |
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