High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation
Hyperglycemia-induced hyperactivity of chemokine CX3CL1 (fractalkine) occurs in the human placenta. Anti-inflammatory/antioxidant activities of resveratrol (3,5,4′-trihydroxy-trans-stilbene) are related to the modulation of chemokine CX3CL1 and its receptor, CX3CR1, signaling pathways. We examined t...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/9853108 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567528004517888 |
|---|---|
| author | Dariusz Szukiewicz Michal Pyzlak Grzegorz Szewczyk Aleksandra Stangret Seweryn Trojanowski Michal Bachanek Wojciech Braksator Jaroslaw Wejman |
| author_facet | Dariusz Szukiewicz Michal Pyzlak Grzegorz Szewczyk Aleksandra Stangret Seweryn Trojanowski Michal Bachanek Wojciech Braksator Jaroslaw Wejman |
| author_sort | Dariusz Szukiewicz |
| collection | DOAJ |
| description | Hyperglycemia-induced hyperactivity of chemokine CX3CL1 (fractalkine) occurs in the human placenta. Anti-inflammatory/antioxidant activities of resveratrol (3,5,4′-trihydroxy-trans-stilbene) are related to the modulation of chemokine CX3CL1 and its receptor, CX3CR1, signaling pathways. We examined the influence of high glucose (25 mmol/L glucose; HG group; N=36) on resveratrol-mediated effects on CX3CL1 and TNF-α production by the placental lobule, CX3CR1 expression and contents of CX3CR1, TNF-α receptor 1 (TNFR1), and NF-κB proteins in placental tissue. The placental lobules perfused under normoglycemic conditions formed the control NG group (N=36). Resveratrol (50 and 100 μM; subgroups B and C) administered into the perfusion fluid lowered the production of both CX3CL1 and TNF-α. The reductions in CX3CL1 levels were more evident in the NG group. CX3CR1 expression was significantly higher in the NG subgroups B and C compared to the HG subgroups B and C (385.2 and 426.5% versus 199.3 and 282.4%, resp.). An increase in CX3CR1 protein content in placental lysates was observed in the NG subgroups B and C. Also, resveratrol significantly decreased NF-κBp65 protein content only in the NG group, not affecting hyperglycemia-elicited TNFR1 upregulation. In conclusion, euglycemia assures optimal effects of resveratrol pertaining to CX3CL1/CX3CR1 signaling in the placenta. Future studies on resveratrol are needed, especially those including maternal-fetal risk assessments. |
| format | Article |
| id | doaj-art-8abe309aa5fa4b02a533c3b9412a23af |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-8abe309aa5fa4b02a533c3b9412a23af2025-02-03T01:01:18ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/98531089853108High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental CirculationDariusz Szukiewicz0Michal Pyzlak1Grzegorz Szewczyk2Aleksandra Stangret3Seweryn Trojanowski4Michal Bachanek5Wojciech Braksator6Jaroslaw Wejman7Department of General & Experimental Pathology with Centre for Preclinical Research and Technology (CEPT), Second Faculty of Medicine, Medical University of Warsaw, ul. Pawinskiego 3C, 02-106 Warsaw, PolandDepartment of General & Experimental Pathology with Centre for Preclinical Research and Technology (CEPT), Second Faculty of Medicine, Medical University of Warsaw, ul. Pawinskiego 3C, 02-106 Warsaw, PolandDepartment of General & Experimental Pathology with Centre for Preclinical Research and Technology (CEPT), Second Faculty of Medicine, Medical University of Warsaw, ul. Pawinskiego 3C, 02-106 Warsaw, PolandDepartment of General & Experimental Pathology with Centre for Preclinical Research and Technology (CEPT), Second Faculty of Medicine, Medical University of Warsaw, ul. Pawinskiego 3C, 02-106 Warsaw, PolandDepartment of Obstetrics & Gynecology, Second Faculty of Medicine, Medical University of Warsaw, ul. Kondratowicza 8, 03-242 Warsaw, PolandDepartment of Obstetrics & Gynecology, Second Faculty of Medicine, Medical University of Warsaw, ul. Kondratowicza 8, 03-242 Warsaw, PolandDepartment of Cardiology, Hypertension, and Internal Medicine, Second Faculty of Medicine, Medical University of Warsaw, ul. Kondratowicza 8, 03-242 Warsaw, PolandDepartment of Pathology, Professor Witold Orlowski Public Clinical Hospital, Medical Center for Postgraduate Education, ul. Czerniakowska 231, 00-416 Warsaw, PolandHyperglycemia-induced hyperactivity of chemokine CX3CL1 (fractalkine) occurs in the human placenta. Anti-inflammatory/antioxidant activities of resveratrol (3,5,4′-trihydroxy-trans-stilbene) are related to the modulation of chemokine CX3CL1 and its receptor, CX3CR1, signaling pathways. We examined the influence of high glucose (25 mmol/L glucose; HG group; N=36) on resveratrol-mediated effects on CX3CL1 and TNF-α production by the placental lobule, CX3CR1 expression and contents of CX3CR1, TNF-α receptor 1 (TNFR1), and NF-κB proteins in placental tissue. The placental lobules perfused under normoglycemic conditions formed the control NG group (N=36). Resveratrol (50 and 100 μM; subgroups B and C) administered into the perfusion fluid lowered the production of both CX3CL1 and TNF-α. The reductions in CX3CL1 levels were more evident in the NG group. CX3CR1 expression was significantly higher in the NG subgroups B and C compared to the HG subgroups B and C (385.2 and 426.5% versus 199.3 and 282.4%, resp.). An increase in CX3CR1 protein content in placental lysates was observed in the NG subgroups B and C. Also, resveratrol significantly decreased NF-κBp65 protein content only in the NG group, not affecting hyperglycemia-elicited TNFR1 upregulation. In conclusion, euglycemia assures optimal effects of resveratrol pertaining to CX3CL1/CX3CR1 signaling in the placenta. Future studies on resveratrol are needed, especially those including maternal-fetal risk assessments.http://dx.doi.org/10.1155/2017/9853108 |
| spellingShingle | Dariusz Szukiewicz Michal Pyzlak Grzegorz Szewczyk Aleksandra Stangret Seweryn Trojanowski Michal Bachanek Wojciech Braksator Jaroslaw Wejman High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation Mediators of Inflammation |
| title | High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation |
| title_full | High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation |
| title_fullStr | High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation |
| title_full_unstemmed | High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation |
| title_short | High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation |
| title_sort | high glucose level disturbs the resveratrol evoked curtailment of cx3cl1 cx3cr1 signaling in human placental circulation |
| url | http://dx.doi.org/10.1155/2017/9853108 |
| work_keys_str_mv | AT dariuszszukiewicz highglucoseleveldisturbstheresveratrolevokedcurtailmentofcx3cl1cx3cr1signalinginhumanplacentalcirculation AT michalpyzlak highglucoseleveldisturbstheresveratrolevokedcurtailmentofcx3cl1cx3cr1signalinginhumanplacentalcirculation AT grzegorzszewczyk highglucoseleveldisturbstheresveratrolevokedcurtailmentofcx3cl1cx3cr1signalinginhumanplacentalcirculation AT aleksandrastangret highglucoseleveldisturbstheresveratrolevokedcurtailmentofcx3cl1cx3cr1signalinginhumanplacentalcirculation AT seweryntrojanowski highglucoseleveldisturbstheresveratrolevokedcurtailmentofcx3cl1cx3cr1signalinginhumanplacentalcirculation AT michalbachanek highglucoseleveldisturbstheresveratrolevokedcurtailmentofcx3cl1cx3cr1signalinginhumanplacentalcirculation AT wojciechbraksator highglucoseleveldisturbstheresveratrolevokedcurtailmentofcx3cl1cx3cr1signalinginhumanplacentalcirculation AT jaroslawwejman highglucoseleveldisturbstheresveratrolevokedcurtailmentofcx3cl1cx3cr1signalinginhumanplacentalcirculation |