Suppressive Activity of a Macrolide Antibiotic, Roxithromycin, on Pro-Inflammatory Cytokine Production in Vitro and in Vivo
This study was designed to examine the influence of a macrolide antibiotic, roxithromycin (RXM), on the production of pro-inflammatory cytokines, interleukin (IL)-1β and tumor necrosis factor (TNF)-α. In the first experiments, we examined the effect of RXM on in vitro cytokine production from lipopo...
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| Format: | Article |
| Language: | English |
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Wiley
1999-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359990351 |
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| author | H. Suzaki K. Asano S. Ohki K. Kanai T. Mizutani T. Hisamitsu |
| author_facet | H. Suzaki K. Asano S. Ohki K. Kanai T. Mizutani T. Hisamitsu |
| author_sort | H. Suzaki |
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| description | This study was designed to examine the influence of a macrolide antibiotic, roxithromycin (RXM), on the production of pro-inflammatory cytokines, interleukin (IL)-1β and tumor necrosis factor (TNF)-α. In the first experiments, we examined the effect of RXM on in vitro cytokine production from lipopolysaccharide (LPS)-stimulated human peripheral blood monocytes. The monocytes were cultured in the presence of various doses of the agent. After 24 h, the culture supernatants were obtained and assayed for IL-1β and TNF-α contents by enzyme-linked immunosorbent assay. RXM suppressed the in vitro production of IL-1β and TNF-α in response to LPS stimulation. This was dose dependent and first noted at a concentration of as little as 0.05 μg/ml, which is much lower than therapeutic blood levels. In the second part of the experiments, we examined the influence of RXM on the appearance of IL-1β and TNF-α in mouse lung extract induced by LPS inhalation. RXM was administered orally into BALB/c mice at a single dose of 2.5 mg/kg once a day for 5-12 weeks. These mice were then instilled with LPS into the trachea and examined for the presence of cytokines in aqueous lung extracts. Pretreatment of mice with RXM for 5 weeks did not influence of the appearance of both IL-1β and TNF-α in aqueous lung extracts. However, pretreatment for more than 7 weeks dramatically suppressed the cytokine appearance in the extracts. |
| format | Article |
| id | doaj-art-8abab462f08f46a5b9f9882d7011198f |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-8abab462f08f46a5b9f9882d7011198f2025-02-03T01:11:21ZengWileyMediators of Inflammation0962-93511466-18611999-01-0184-519920410.1080/09629359990351Suppressive Activity of a Macrolide Antibiotic, Roxithromycin, on Pro-Inflammatory Cytokine Production in Vitro and in VivoH. Suzaki0K. Asano1S. Ohki2K. Kanai3T. Mizutani4T. Hisamitsu5Department of Otolaryngology, School of Medicine, Showa University, 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142–8555, JapanDepartment of Physiology, School of Medicine, Showa University, 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142–8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142–8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142–8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142–8555, JapanDepartment of Physiology, School of Medicine, Showa University, 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142–8555, JapanThis study was designed to examine the influence of a macrolide antibiotic, roxithromycin (RXM), on the production of pro-inflammatory cytokines, interleukin (IL)-1β and tumor necrosis factor (TNF)-α. In the first experiments, we examined the effect of RXM on in vitro cytokine production from lipopolysaccharide (LPS)-stimulated human peripheral blood monocytes. The monocytes were cultured in the presence of various doses of the agent. After 24 h, the culture supernatants were obtained and assayed for IL-1β and TNF-α contents by enzyme-linked immunosorbent assay. RXM suppressed the in vitro production of IL-1β and TNF-α in response to LPS stimulation. This was dose dependent and first noted at a concentration of as little as 0.05 μg/ml, which is much lower than therapeutic blood levels. In the second part of the experiments, we examined the influence of RXM on the appearance of IL-1β and TNF-α in mouse lung extract induced by LPS inhalation. RXM was administered orally into BALB/c mice at a single dose of 2.5 mg/kg once a day for 5-12 weeks. These mice were then instilled with LPS into the trachea and examined for the presence of cytokines in aqueous lung extracts. Pretreatment of mice with RXM for 5 weeks did not influence of the appearance of both IL-1β and TNF-α in aqueous lung extracts. However, pretreatment for more than 7 weeks dramatically suppressed the cytokine appearance in the extracts.http://dx.doi.org/10.1080/09629359990351RoxithromycinInterleukin-1βTumor necrosis factor-αInhibitionMouseIn vivoIn vitro. |
| spellingShingle | H. Suzaki K. Asano S. Ohki K. Kanai T. Mizutani T. Hisamitsu Suppressive Activity of a Macrolide Antibiotic, Roxithromycin, on Pro-Inflammatory Cytokine Production in Vitro and in Vivo Mediators of Inflammation Roxithromycin Interleukin-1β Tumor necrosis factor-α Inhibition Mouse In vivo In vitro. |
| title | Suppressive Activity of a Macrolide Antibiotic, Roxithromycin, on Pro-Inflammatory Cytokine Production in Vitro and in Vivo |
| title_full | Suppressive Activity of a Macrolide Antibiotic, Roxithromycin, on Pro-Inflammatory Cytokine Production in Vitro and in Vivo |
| title_fullStr | Suppressive Activity of a Macrolide Antibiotic, Roxithromycin, on Pro-Inflammatory Cytokine Production in Vitro and in Vivo |
| title_full_unstemmed | Suppressive Activity of a Macrolide Antibiotic, Roxithromycin, on Pro-Inflammatory Cytokine Production in Vitro and in Vivo |
| title_short | Suppressive Activity of a Macrolide Antibiotic, Roxithromycin, on Pro-Inflammatory Cytokine Production in Vitro and in Vivo |
| title_sort | suppressive activity of a macrolide antibiotic roxithromycin on pro inflammatory cytokine production in vitro and in vivo |
| topic | Roxithromycin Interleukin-1β Tumor necrosis factor-α Inhibition Mouse In vivo In vitro. |
| url | http://dx.doi.org/10.1080/09629359990351 |
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