IL1RN and KRT13 Expression in Bladder Cancer: Association with Pathologic Characteristics and Smoking Status
Purpose. To validate microarray data on cytokeratin 13 (KRT13) and interleukin-1 receptor antagonist (IL1RN) expression in urothelial carcinoma of the urinary bladder (UCB) and to correlate our findings with pathologic characteristics and tobacco smoking. Methods. UCB tissue samples (n=109) and con...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Advances in Urology |
| Online Access: | http://dx.doi.org/10.1155/2014/184602 |
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| author | Thomas S. Worst Verena Reiner Ute Gabriel Christel Weiß Philipp Erben Thomas Martini Christian Bolenz |
| author_facet | Thomas S. Worst Verena Reiner Ute Gabriel Christel Weiß Philipp Erben Thomas Martini Christian Bolenz |
| author_sort | Thomas S. Worst |
| collection | DOAJ |
| description | Purpose. To validate microarray data on cytokeratin 13 (KRT13) and interleukin-1 receptor antagonist (IL1RN) expression in urothelial carcinoma of the urinary bladder (UCB) and to correlate our findings with pathologic characteristics and tobacco smoking.
Methods. UCB tissue samples (n=109) and control samples (n=14) were obtained from transurethral resection and radical cystectomy specimens. Immunohistochemical staining of KRT13 and IL1RN was performed and semiquantitative expression scores were assessed. Smoking status was evaluated using a standardized questionnaire. Expression scores were correlated with pathologic characteristics (tumor stage and grade) and with smoking status. Results. Loss of KRT13 and IL1RN expression was observed in UCB tissue samples when compared to controls (P=0.007, P=0.008) in which KRT13 and IL1RN expression were high. IL1RN expression was significantly reduced in muscle-invasive tumors (P=0.003). In tissue samples of current smokers, a significant downregulation of IL1RN was found when compared to never smokers (P=0.013). Conclusion. Decreased expressions of KRT13 and IL1RN are common features of UCB and are associated with aggressive disease. Tobacco smoking may enhance the loss of IL1RN, indicating an overweight of proinflammatory mediators involved in UCB progression. Further validation of the influence of smoking on IL1RN expression is warranted. |
| format | Article |
| id | doaj-art-8ab53006765d493c807a77e87eac9fd2 |
| institution | Kabale University |
| issn | 1687-6369 1687-6377 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Urology |
| spelling | doaj-art-8ab53006765d493c807a77e87eac9fd22025-02-03T07:26:18ZengWileyAdvances in Urology1687-63691687-63772014-01-01201410.1155/2014/184602184602IL1RN and KRT13 Expression in Bladder Cancer: Association with Pathologic Characteristics and Smoking StatusThomas S. Worst0Verena Reiner1Ute Gabriel2Christel Weiß3Philipp Erben4Thomas Martini5Christian Bolenz6Department of Urology, Mannheim Medical Center, University of Heidelberg, Theodor-Kutzer-Ufer 1–3, 68167 Mannheim, GermanyDepartment of Urology, Mannheim Medical Center, University of Heidelberg, Theodor-Kutzer-Ufer 1–3, 68167 Mannheim, GermanyDepartment of Urology, Mannheim Medical Center, University of Heidelberg, Theodor-Kutzer-Ufer 1–3, 68167 Mannheim, GermanyDepartment for Statistical Analysis, Mannheim Medical Center, University of Heidelberg, Mannheim, GermanyDepartment of Urology, Mannheim Medical Center, University of Heidelberg, Theodor-Kutzer-Ufer 1–3, 68167 Mannheim, GermanyDepartment of Urology, Mannheim Medical Center, University of Heidelberg, Theodor-Kutzer-Ufer 1–3, 68167 Mannheim, GermanyDepartment of Urology, Mannheim Medical Center, University of Heidelberg, Theodor-Kutzer-Ufer 1–3, 68167 Mannheim, GermanyPurpose. To validate microarray data on cytokeratin 13 (KRT13) and interleukin-1 receptor antagonist (IL1RN) expression in urothelial carcinoma of the urinary bladder (UCB) and to correlate our findings with pathologic characteristics and tobacco smoking. Methods. UCB tissue samples (n=109) and control samples (n=14) were obtained from transurethral resection and radical cystectomy specimens. Immunohistochemical staining of KRT13 and IL1RN was performed and semiquantitative expression scores were assessed. Smoking status was evaluated using a standardized questionnaire. Expression scores were correlated with pathologic characteristics (tumor stage and grade) and with smoking status. Results. Loss of KRT13 and IL1RN expression was observed in UCB tissue samples when compared to controls (P=0.007, P=0.008) in which KRT13 and IL1RN expression were high. IL1RN expression was significantly reduced in muscle-invasive tumors (P=0.003). In tissue samples of current smokers, a significant downregulation of IL1RN was found when compared to never smokers (P=0.013). Conclusion. Decreased expressions of KRT13 and IL1RN are common features of UCB and are associated with aggressive disease. Tobacco smoking may enhance the loss of IL1RN, indicating an overweight of proinflammatory mediators involved in UCB progression. Further validation of the influence of smoking on IL1RN expression is warranted.http://dx.doi.org/10.1155/2014/184602 |
| spellingShingle | Thomas S. Worst Verena Reiner Ute Gabriel Christel Weiß Philipp Erben Thomas Martini Christian Bolenz IL1RN and KRT13 Expression in Bladder Cancer: Association with Pathologic Characteristics and Smoking Status Advances in Urology |
| title | IL1RN and KRT13 Expression in Bladder Cancer: Association with Pathologic Characteristics and Smoking Status |
| title_full | IL1RN and KRT13 Expression in Bladder Cancer: Association with Pathologic Characteristics and Smoking Status |
| title_fullStr | IL1RN and KRT13 Expression in Bladder Cancer: Association with Pathologic Characteristics and Smoking Status |
| title_full_unstemmed | IL1RN and KRT13 Expression in Bladder Cancer: Association with Pathologic Characteristics and Smoking Status |
| title_short | IL1RN and KRT13 Expression in Bladder Cancer: Association with Pathologic Characteristics and Smoking Status |
| title_sort | il1rn and krt13 expression in bladder cancer association with pathologic characteristics and smoking status |
| url | http://dx.doi.org/10.1155/2014/184602 |
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