<i>Microdochium majus</i> Isolated from Grapevine Is a Mycoparasite of <i>Botrytis cinerea</i>
The best known <i>Microdochium</i> spp. are important pathogens of small-grain cereals and/or endophytes of diverse monocot hosts. This study is the first report of <i>M. majus</i> isolated from asymptomatic grapevine tissues. It was hypothesised that this <i>M. majus&l...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/1/31 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588213347155968 |
|---|---|
| author | Kálmán Zoltán Váczy Dóra Szabó Nikolett Molnár Tibor Kiss Levente Kiss Yu Pei Tan Ádám Novák Xénia Pálfi Adrienn Gomba-Tóth Zoltán Karácsony |
| author_facet | Kálmán Zoltán Váczy Dóra Szabó Nikolett Molnár Tibor Kiss Levente Kiss Yu Pei Tan Ádám Novák Xénia Pálfi Adrienn Gomba-Tóth Zoltán Karácsony |
| author_sort | Kálmán Zoltán Váczy |
| collection | DOAJ |
| description | The best known <i>Microdochium</i> spp. are important pathogens of small-grain cereals and/or endophytes of diverse monocot hosts. This study is the first report of <i>M. majus</i> isolated from asymptomatic grapevine tissues. It was hypothesised that this <i>M. majus</i> strain, CBS 152328, was an endophyte and an antagonist of some fungal pathogens of grapevine. Microscopic examinations revealed that this strain was a necrotrophic mycoparasite of <i>Botrytis cinerea</i>. This was demonstrated in the confrontation zones of dual cultures of <i>M. majus</i> and <i>B. cinerea</i>, and also on the surface of co-inoculated grape leaf discs and germinated wheat grains. Pathogenicity tests indicated that <i>M. majus</i> can colonise both grape leaf discs and germinated wheat, but it only damaged wheat. When co-inoculated with <i>B. cinerea</i> onto grape leaf discs, the <i>M. majus</i> strain CBS 152328 suppressed its mycohost on grape tissues and prevented leaf necrosis caused by <i>B. cinerea</i>. In addition to the parasitism, <i>M. majus</i> also showed mild antibiosis against <i>B. cinerea</i>, as well as a defence elicitor effect on grape leaf discs. This work is the first report of the mycoparasitic behaviour of <i>M. majus</i>, in addition to its first isolation from a dicot host. |
| format | Article |
| id | doaj-art-8a90bdc3217c4a429a786c8b85c2bb44 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-8a90bdc3217c4a429a786c8b85c2bb442025-01-24T13:37:17ZengMDPI AGJournal of Fungi2309-608X2025-01-011113110.3390/jof11010031<i>Microdochium majus</i> Isolated from Grapevine Is a Mycoparasite of <i>Botrytis cinerea</i>Kálmán Zoltán Váczy0Dóra Szabó1Nikolett Molnár2Tibor Kiss3Levente Kiss4Yu Pei Tan5Ádám Novák6Xénia Pálfi7Adrienn Gomba-Tóth8Zoltán Karácsony9Food and Wine Research Institute, Eszterházy Károly Catholic University, Leányka utca 8/G, H-3300 Eger, HungaryFood and Wine Research Institute, Eszterházy Károly Catholic University, Leányka utca 8/G, H-3300 Eger, HungaryFood and Wine Research Institute, Eszterházy Károly Catholic University, Leányka utca 8/G, H-3300 Eger, HungaryFood and Wine Research Institute, Eszterházy Károly Catholic University, Leányka utca 8/G, H-3300 Eger, HungaryFood and Wine Research Institute, Eszterházy Károly Catholic University, Leányka utca 8/G, H-3300 Eger, HungaryCentre for Crop Health, University of Southern Queensland, Toowoomba, QLD 4350, AustraliaFood and Wine Research Institute, Eszterházy Károly Catholic University, Leányka utca 8/G, H-3300 Eger, HungaryFood and Wine Research Institute, Eszterházy Károly Catholic University, Leányka utca 8/G, H-3300 Eger, HungaryFood and Wine Research Institute, Eszterházy Károly Catholic University, Leányka utca 8/G, H-3300 Eger, HungaryFood and Wine Research Institute, Eszterházy Károly Catholic University, Leányka utca 8/G, H-3300 Eger, HungaryThe best known <i>Microdochium</i> spp. are important pathogens of small-grain cereals and/or endophytes of diverse monocot hosts. This study is the first report of <i>M. majus</i> isolated from asymptomatic grapevine tissues. It was hypothesised that this <i>M. majus</i> strain, CBS 152328, was an endophyte and an antagonist of some fungal pathogens of grapevine. Microscopic examinations revealed that this strain was a necrotrophic mycoparasite of <i>Botrytis cinerea</i>. This was demonstrated in the confrontation zones of dual cultures of <i>M. majus</i> and <i>B. cinerea</i>, and also on the surface of co-inoculated grape leaf discs and germinated wheat grains. Pathogenicity tests indicated that <i>M. majus</i> can colonise both grape leaf discs and germinated wheat, but it only damaged wheat. When co-inoculated with <i>B. cinerea</i> onto grape leaf discs, the <i>M. majus</i> strain CBS 152328 suppressed its mycohost on grape tissues and prevented leaf necrosis caused by <i>B. cinerea</i>. In addition to the parasitism, <i>M. majus</i> also showed mild antibiosis against <i>B. cinerea</i>, as well as a defence elicitor effect on grape leaf discs. This work is the first report of the mycoparasitic behaviour of <i>M. majus</i>, in addition to its first isolation from a dicot host.https://www.mdpi.com/2309-608X/11/1/31biocontrolendophytefungal parasitehost preference |
| spellingShingle | Kálmán Zoltán Váczy Dóra Szabó Nikolett Molnár Tibor Kiss Levente Kiss Yu Pei Tan Ádám Novák Xénia Pálfi Adrienn Gomba-Tóth Zoltán Karácsony <i>Microdochium majus</i> Isolated from Grapevine Is a Mycoparasite of <i>Botrytis cinerea</i> Journal of Fungi biocontrol endophyte fungal parasite host preference |
| title | <i>Microdochium majus</i> Isolated from Grapevine Is a Mycoparasite of <i>Botrytis cinerea</i> |
| title_full | <i>Microdochium majus</i> Isolated from Grapevine Is a Mycoparasite of <i>Botrytis cinerea</i> |
| title_fullStr | <i>Microdochium majus</i> Isolated from Grapevine Is a Mycoparasite of <i>Botrytis cinerea</i> |
| title_full_unstemmed | <i>Microdochium majus</i> Isolated from Grapevine Is a Mycoparasite of <i>Botrytis cinerea</i> |
| title_short | <i>Microdochium majus</i> Isolated from Grapevine Is a Mycoparasite of <i>Botrytis cinerea</i> |
| title_sort | i microdochium majus i isolated from grapevine is a mycoparasite of i botrytis cinerea i |
| topic | biocontrol endophyte fungal parasite host preference |
| url | https://www.mdpi.com/2309-608X/11/1/31 |
| work_keys_str_mv | AT kalmanzoltanvaczy imicrodochiummajusiisolatedfromgrapevineisamycoparasiteofibotrytiscinereai AT doraszabo imicrodochiummajusiisolatedfromgrapevineisamycoparasiteofibotrytiscinereai AT nikolettmolnar imicrodochiummajusiisolatedfromgrapevineisamycoparasiteofibotrytiscinereai AT tiborkiss imicrodochiummajusiisolatedfromgrapevineisamycoparasiteofibotrytiscinereai AT leventekiss imicrodochiummajusiisolatedfromgrapevineisamycoparasiteofibotrytiscinereai AT yupeitan imicrodochiummajusiisolatedfromgrapevineisamycoparasiteofibotrytiscinereai AT adamnovak imicrodochiummajusiisolatedfromgrapevineisamycoparasiteofibotrytiscinereai AT xeniapalfi imicrodochiummajusiisolatedfromgrapevineisamycoparasiteofibotrytiscinereai AT adrienngombatoth imicrodochiummajusiisolatedfromgrapevineisamycoparasiteofibotrytiscinereai AT zoltankaracsony imicrodochiummajusiisolatedfromgrapevineisamycoparasiteofibotrytiscinereai |