Molecular networking-assisted flavonoid profile of Gypsophila glomerata extract in relation to its protective effects on carbon tetrachloride-induced hepatorenal damage in rats
The aim of the study was to provide an in-depth characterization of the methanol-aqueous extract from the aerial parts of Gypsophila glomerata Pall. Ex Adams (Caryophyllaceae) (EGG) and to assess its protective potential on carbon tetrachloride (CCl4)-induced liver and kidney damage in male Wistar r...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2022-03-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2022-0009 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832573981360652288 |
|---|---|
| author | Dimitrova Bojana Vitanska Rumyana Gevrenova Reneta Zheleva-Dimitrova Dimitrina Balabanova Vessela Stoev Stoycho |
| author_facet | Dimitrova Bojana Vitanska Rumyana Gevrenova Reneta Zheleva-Dimitrova Dimitrina Balabanova Vessela Stoev Stoycho |
| author_sort | Dimitrova Bojana |
| collection | DOAJ |
| description | The aim of the study was to provide an in-depth characterization of the methanol-aqueous extract from the aerial parts of Gypsophila glomerata Pall. Ex Adams (Caryophyllaceae) (EGG) and to assess its protective potential on carbon tetrachloride (CCl4)-induced liver and kidney damage in male Wistar rats. Twenty-two flavonoid C-, O- and C,O--glycosides in EGG were annotated by mass spectrometry--based molecular networking; nine of them are reported in this species for the first time. Fourteen-day oral administration of EGG at a dose 200 mg kg−1 bm prevented significantly CCl4-induced liver injury, discerned by an amelioration of the markers of oxidative stress (GSH and MDA) and transaminase activity. EGG decreased the serum level of urea and creatinine as well. The observed improvement of biochemical parameters was supported by histopathological observations. The protective hepatorenal effects of EGG, rich in 2“-О-pentosyl-6-С-hexosyl-apigenin/luteolin/ methylluteolin and their acetyl- and methoxycinnamoyl-derivatives, were comparable with the effects of the positive control silymarin. |
| format | Article |
| id | doaj-art-8a87e3da7841483bb673ec60e0582089 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-8a87e3da7841483bb673ec60e05820892025-02-02T01:41:29ZengSciendoActa Pharmaceutica1846-95582022-03-01721597710.2478/acph-2022-0009Molecular networking-assisted flavonoid profile of Gypsophila glomerata extract in relation to its protective effects on carbon tetrachloride-induced hepatorenal damage in ratsDimitrova Bojana0Vitanska Rumyana1Gevrenova Reneta2Zheleva-Dimitrova Dimitrina3Balabanova Vessela4Stoev Stoycho5Department of Pharmacology Pharmacotherapy and Toxicology Faculty of Pharmacy, Medical University of Sofia, 1000 SofiaDepartment of Pharmacology Pharmacotherapy and Toxicology Faculty of Pharmacy, Medical University of Sofia, 1000 SofiaDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Sofia1000 SofiaDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Sofia1000 SofiaDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Sofia1000 SofiaDepartment of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Students Campus, 6000 Stara Zagora, BulgariaThe aim of the study was to provide an in-depth characterization of the methanol-aqueous extract from the aerial parts of Gypsophila glomerata Pall. Ex Adams (Caryophyllaceae) (EGG) and to assess its protective potential on carbon tetrachloride (CCl4)-induced liver and kidney damage in male Wistar rats. Twenty-two flavonoid C-, O- and C,O--glycosides in EGG were annotated by mass spectrometry--based molecular networking; nine of them are reported in this species for the first time. Fourteen-day oral administration of EGG at a dose 200 mg kg−1 bm prevented significantly CCl4-induced liver injury, discerned by an amelioration of the markers of oxidative stress (GSH and MDA) and transaminase activity. EGG decreased the serum level of urea and creatinine as well. The observed improvement of biochemical parameters was supported by histopathological observations. The protective hepatorenal effects of EGG, rich in 2“-О-pentosyl-6-С-hexosyl-apigenin/luteolin/ methylluteolin and their acetyl- and methoxycinnamoyl-derivatives, were comparable with the effects of the positive control silymarin.https://doi.org/10.2478/acph-2022-0009gypsophila glomerataccl4molecular networkhepatorenal toxicityflavonoids |
| spellingShingle | Dimitrova Bojana Vitanska Rumyana Gevrenova Reneta Zheleva-Dimitrova Dimitrina Balabanova Vessela Stoev Stoycho Molecular networking-assisted flavonoid profile of Gypsophila glomerata extract in relation to its protective effects on carbon tetrachloride-induced hepatorenal damage in rats Acta Pharmaceutica gypsophila glomerata ccl4 molecular network hepatorenal toxicity flavonoids |
| title | Molecular networking-assisted flavonoid profile of Gypsophila glomerata extract in relation to its protective effects on carbon tetrachloride-induced hepatorenal damage in rats |
| title_full | Molecular networking-assisted flavonoid profile of Gypsophila glomerata extract in relation to its protective effects on carbon tetrachloride-induced hepatorenal damage in rats |
| title_fullStr | Molecular networking-assisted flavonoid profile of Gypsophila glomerata extract in relation to its protective effects on carbon tetrachloride-induced hepatorenal damage in rats |
| title_full_unstemmed | Molecular networking-assisted flavonoid profile of Gypsophila glomerata extract in relation to its protective effects on carbon tetrachloride-induced hepatorenal damage in rats |
| title_short | Molecular networking-assisted flavonoid profile of Gypsophila glomerata extract in relation to its protective effects on carbon tetrachloride-induced hepatorenal damage in rats |
| title_sort | molecular networking assisted flavonoid profile of gypsophila glomerata extract in relation to its protective effects on carbon tetrachloride induced hepatorenal damage in rats |
| topic | gypsophila glomerata ccl4 molecular network hepatorenal toxicity flavonoids |
| url | https://doi.org/10.2478/acph-2022-0009 |
| work_keys_str_mv | AT dimitrovabojana molecularnetworkingassistedflavonoidprofileofgypsophilaglomerataextractinrelationtoitsprotectiveeffectsoncarbontetrachlorideinducedhepatorenaldamageinrats AT vitanskarumyana molecularnetworkingassistedflavonoidprofileofgypsophilaglomerataextractinrelationtoitsprotectiveeffectsoncarbontetrachlorideinducedhepatorenaldamageinrats AT gevrenovareneta molecularnetworkingassistedflavonoidprofileofgypsophilaglomerataextractinrelationtoitsprotectiveeffectsoncarbontetrachlorideinducedhepatorenaldamageinrats AT zhelevadimitrovadimitrina molecularnetworkingassistedflavonoidprofileofgypsophilaglomerataextractinrelationtoitsprotectiveeffectsoncarbontetrachlorideinducedhepatorenaldamageinrats AT balabanovavessela molecularnetworkingassistedflavonoidprofileofgypsophilaglomerataextractinrelationtoitsprotectiveeffectsoncarbontetrachlorideinducedhepatorenaldamageinrats AT stoevstoycho molecularnetworkingassistedflavonoidprofileofgypsophilaglomerataextractinrelationtoitsprotectiveeffectsoncarbontetrachlorideinducedhepatorenaldamageinrats |