Proteomic, metabolomic and lipidomic profiles in community acquired pneumonia for differentiating viral and bacterial infections
Abstract Community-acquired pneumonia (CAP) has a significant impact on public health, especially in light of the recent SARS-CoV-2 pandemic. To enhance disease characterization and improve understanding of the underlying mechanisms, a comprehensive analysis of the plasma lipidome, metabolome and pr...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-85229-2 |
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| author | Samuel Rischke Robert Gurke Ann-Sophie Zielbauer Nicole Ziegler Lisa Hahnefeld Michaela Köhm Aimo Kannt Maria JGT Vehreschild Gerd Geisslinger Gernot Rohde Carla Bellinghausen Frank Behrens CAPNETZ Study group |
| author_facet | Samuel Rischke Robert Gurke Ann-Sophie Zielbauer Nicole Ziegler Lisa Hahnefeld Michaela Köhm Aimo Kannt Maria JGT Vehreschild Gerd Geisslinger Gernot Rohde Carla Bellinghausen Frank Behrens CAPNETZ Study group |
| author_sort | Samuel Rischke |
| collection | DOAJ |
| description | Abstract Community-acquired pneumonia (CAP) has a significant impact on public health, especially in light of the recent SARS-CoV-2 pandemic. To enhance disease characterization and improve understanding of the underlying mechanisms, a comprehensive analysis of the plasma lipidome, metabolome and proteome was conducted in patients with viral and bacterial CAP infections, including those induced by SARS-CoV-2. Lipidomic, metabolomic and proteomic profiling were conducted on plasma samples of 69 patients suffering either from viral or bacterial CAP. Lipid and metabolite analyses were LC-MS-based, while proteomic analyses were performed using multiple panels of the Olink platform. Statistical methods, machine learning and pathway analyses were conducted investigating differences between the infection types. Through comparison of the bacterial and viral pathogen groups, distinct signatures were observed in the plasma profiles. Notably, linoleic acid-derived inflammation signaling metabolites (EpOME and DiHOME) were increased in viral CAP compared to bacterial CAP. Similarly, proteins involved in cellular immune response and apoptosis (LAG-3 and TRAIL) showed elevated levels in viral CAP, while bacterial CAP exhibited notable elevation in pattern-recognizing receptors (CLEC4D and EN-RAGE). Additionally, within the lipidomic profile at baseline, several lipids displayed notable differences between viral and bacterial pneumonia, including bile acids (GCA, TCA, TCDCA), various tri- and diglycerides (TGs and DGs), and several phosphatidylcholines (PCs). These findings hold promise for facilitating the differential diagnosis of viral and bacterial pulmonary infections based on the systemic lipidome, metabolome and proteome, enabling timely treatment decisions. Additionally, they highlight potential targets for drug research, advancing therapeutic interventions in CAP. By providing valuable insights into the molecular characterization of CAP, this study contributes to the improvement of understanding the disease and, ultimately, the development of effective treatment strategies. |
| format | Article |
| id | doaj-art-8a6f3155dc274a1aacc69ced840a86f2 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-8a6f3155dc274a1aacc69ced840a86f22025-01-19T12:19:44ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-85229-2Proteomic, metabolomic and lipidomic profiles in community acquired pneumonia for differentiating viral and bacterial infectionsSamuel Rischke0Robert Gurke1Ann-Sophie Zielbauer2Nicole Ziegler3Lisa Hahnefeld4Michaela Köhm5Aimo Kannt6Maria JGT Vehreschild7Gerd Geisslinger8Gernot Rohde9Carla Bellinghausen10Frank Behrens11CAPNETZ Study groupInstitute of Clinical Pharmacology, Faculty of Medicine, Goethe University FrankfurtInstitute of Clinical Pharmacology, Faculty of Medicine, Goethe University FrankfurtDepartment of Internal Medicine, Infectious Diseases, University Hospital, Goethe University FrankfurtFraunhofer Institute for Translational Medicine and Pharmacology (ITMP)Institute of Clinical Pharmacology, Faculty of Medicine, Goethe University FrankfurtFraunhofer Institute for Translational Medicine and Pharmacology (ITMP)Institute of Clinical Pharmacology, Faculty of Medicine, Goethe University FrankfurtFraunhofer Institute for Translational Medicine and Pharmacology (ITMP)Institute of Clinical Pharmacology, Faculty of Medicine, Goethe University FrankfurtDepartment of Respiratory Medicine, Medical Clinic I, University Hospital, Goethe University FrankfurtDepartment of Respiratory Medicine, Medical Clinic I, University Hospital, Goethe University FrankfurtFraunhofer Institute for Translational Medicine and Pharmacology (ITMP)Abstract Community-acquired pneumonia (CAP) has a significant impact on public health, especially in light of the recent SARS-CoV-2 pandemic. To enhance disease characterization and improve understanding of the underlying mechanisms, a comprehensive analysis of the plasma lipidome, metabolome and proteome was conducted in patients with viral and bacterial CAP infections, including those induced by SARS-CoV-2. Lipidomic, metabolomic and proteomic profiling were conducted on plasma samples of 69 patients suffering either from viral or bacterial CAP. Lipid and metabolite analyses were LC-MS-based, while proteomic analyses were performed using multiple panels of the Olink platform. Statistical methods, machine learning and pathway analyses were conducted investigating differences between the infection types. Through comparison of the bacterial and viral pathogen groups, distinct signatures were observed in the plasma profiles. Notably, linoleic acid-derived inflammation signaling metabolites (EpOME and DiHOME) were increased in viral CAP compared to bacterial CAP. Similarly, proteins involved in cellular immune response and apoptosis (LAG-3 and TRAIL) showed elevated levels in viral CAP, while bacterial CAP exhibited notable elevation in pattern-recognizing receptors (CLEC4D and EN-RAGE). Additionally, within the lipidomic profile at baseline, several lipids displayed notable differences between viral and bacterial pneumonia, including bile acids (GCA, TCA, TCDCA), various tri- and diglycerides (TGs and DGs), and several phosphatidylcholines (PCs). These findings hold promise for facilitating the differential diagnosis of viral and bacterial pulmonary infections based on the systemic lipidome, metabolome and proteome, enabling timely treatment decisions. Additionally, they highlight potential targets for drug research, advancing therapeutic interventions in CAP. By providing valuable insights into the molecular characterization of CAP, this study contributes to the improvement of understanding the disease and, ultimately, the development of effective treatment strategies.https://doi.org/10.1038/s41598-025-85229-2 |
| spellingShingle | Samuel Rischke Robert Gurke Ann-Sophie Zielbauer Nicole Ziegler Lisa Hahnefeld Michaela Köhm Aimo Kannt Maria JGT Vehreschild Gerd Geisslinger Gernot Rohde Carla Bellinghausen Frank Behrens CAPNETZ Study group Proteomic, metabolomic and lipidomic profiles in community acquired pneumonia for differentiating viral and bacterial infections Scientific Reports |
| title | Proteomic, metabolomic and lipidomic profiles in community acquired pneumonia for differentiating viral and bacterial infections |
| title_full | Proteomic, metabolomic and lipidomic profiles in community acquired pneumonia for differentiating viral and bacterial infections |
| title_fullStr | Proteomic, metabolomic and lipidomic profiles in community acquired pneumonia for differentiating viral and bacterial infections |
| title_full_unstemmed | Proteomic, metabolomic and lipidomic profiles in community acquired pneumonia for differentiating viral and bacterial infections |
| title_short | Proteomic, metabolomic and lipidomic profiles in community acquired pneumonia for differentiating viral and bacterial infections |
| title_sort | proteomic metabolomic and lipidomic profiles in community acquired pneumonia for differentiating viral and bacterial infections |
| url | https://doi.org/10.1038/s41598-025-85229-2 |
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