Isolation, purification and characterization of an anaerobic methane oxidizer
An anaerobic bacterial isolate capable of oxidizing methane anaerobically was isolated from a rice soil developed from coastal saline soil and was named Strain AMOB3. The cells of this strain were rod-shaped with a size of (0.3-0.5) µm × (0.7-0.8) μm, without flagella, without mobility, and Gram-neg...
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Zhejiang University Press
2002-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2002.06.0619 |
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| author | MIN Hang TAN Yu-long WU Wei-xiang CHEN Zhong-yun CHEN Mei-ci |
| author_facet | MIN Hang TAN Yu-long WU Wei-xiang CHEN Zhong-yun CHEN Mei-ci |
| author_sort | MIN Hang |
| collection | DOAJ |
| description | An anaerobic bacterial isolate capable of oxidizing methane anaerobically was isolated from a rice soil developed from coastal saline soil and was named Strain AMOB3. The cells of this strain were rod-shaped with a size of (0.3-0.5) µm × (0.7-0.8) μm, without flagella, without mobility, and Gram-negative. The colonies of the strain formed on anaerobic agar medium were very small, with diameter of 0.2 mm. The strain could utilize methane, methanol fairly well but use formate ineffectively. Glucose, sucrose, malatose, lactose, methylamine, dimethylamine and trimethylamine etc. was not metabolized; NH<sub>4</sub><sup>+</sup>-N, but not NO<sub>3</sub><sup>-</sup>, could be used as the nitrogen source. The optimum pH of culture medium was 6.5 with an effective range from 5.5 to 7.5 and the optimum temperature was 30 C ranged from 25 C to 35 C. The cell of the strain possessed a membrane running around peripheral cytoplasm, which was similar to structure of the type Ⅱ membrane in aerobic methane oxidizers. The methane oxidizing ability of the strain was confirmed by gas chromatographic analysis of the gas phase from the culture tube. It was considered that Strain AMOB3 might be a new species of methane oxidizer which could anaerobically utilize methane, based on comparison of its bio-characteristics and other aerobic methane oxidizers. |
| format | Article |
| id | doaj-art-8a6736a5eec34ca79a024ea48e39236a |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2002-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-8a6736a5eec34ca79a024ea48e39236a2025-08-20T02:54:50ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552002-11-012861962410.3785/1008-9209.2002.06.061910089209Isolation, purification and characterization of an anaerobic methane oxidizerMIN HangTAN Yu-longWU Wei-xiangCHEN Zhong-yunCHEN Mei-ciAn anaerobic bacterial isolate capable of oxidizing methane anaerobically was isolated from a rice soil developed from coastal saline soil and was named Strain AMOB3. The cells of this strain were rod-shaped with a size of (0.3-0.5) µm × (0.7-0.8) μm, without flagella, without mobility, and Gram-negative. The colonies of the strain formed on anaerobic agar medium were very small, with diameter of 0.2 mm. The strain could utilize methane, methanol fairly well but use formate ineffectively. Glucose, sucrose, malatose, lactose, methylamine, dimethylamine and trimethylamine etc. was not metabolized; NH<sub>4</sub><sup>+</sup>-N, but not NO<sub>3</sub><sup>-</sup>, could be used as the nitrogen source. The optimum pH of culture medium was 6.5 with an effective range from 5.5 to 7.5 and the optimum temperature was 30 C ranged from 25 C to 35 C. The cell of the strain possessed a membrane running around peripheral cytoplasm, which was similar to structure of the type Ⅱ membrane in aerobic methane oxidizers. The methane oxidizing ability of the strain was confirmed by gas chromatographic analysis of the gas phase from the culture tube. It was considered that Strain AMOB3 might be a new species of methane oxidizer which could anaerobically utilize methane, based on comparison of its bio-characteristics and other aerobic methane oxidizers.https://www.academax.com/doi/10.3785/1008-9209.2002.06.0619methane oxidationanaerobic methane oxidationmethane oxidizeranaerobic methane oxidizer |
| spellingShingle | MIN Hang TAN Yu-long WU Wei-xiang CHEN Zhong-yun CHEN Mei-ci Isolation, purification and characterization of an anaerobic methane oxidizer 浙江大学学报. 农业与生命科学版 methane oxidation anaerobic methane oxidation methane oxidizer anaerobic methane oxidizer |
| title | Isolation, purification and characterization of an anaerobic methane oxidizer |
| title_full | Isolation, purification and characterization of an anaerobic methane oxidizer |
| title_fullStr | Isolation, purification and characterization of an anaerobic methane oxidizer |
| title_full_unstemmed | Isolation, purification and characterization of an anaerobic methane oxidizer |
| title_short | Isolation, purification and characterization of an anaerobic methane oxidizer |
| title_sort | isolation purification and characterization of an anaerobic methane oxidizer |
| topic | methane oxidation anaerobic methane oxidation methane oxidizer anaerobic methane oxidizer |
| url | https://www.academax.com/doi/10.3785/1008-9209.2002.06.0619 |
| work_keys_str_mv | AT minhang isolationpurificationandcharacterizationofananaerobicmethaneoxidizer AT tanyulong isolationpurificationandcharacterizationofananaerobicmethaneoxidizer AT wuweixiang isolationpurificationandcharacterizationofananaerobicmethaneoxidizer AT chenzhongyun isolationpurificationandcharacterizationofananaerobicmethaneoxidizer AT chenmeici isolationpurificationandcharacterizationofananaerobicmethaneoxidizer |