Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes
Abstract Mycoplasma pneumonia, caused by Mycoplasma bovis (Mycoplasmopsis bovis; M. bovis), is linked with severe inflammatory reactions in the lungs and can be challenging to treat with antibiotics. Biofilms play a significant role in bacterial persistence and contribute to the development of chron...
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| Language: | English |
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BMC
2025-01-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-025-01468-1 |
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| author | Koji Nishi Satoshi Gondaira Yuki Hirano Masahide Ohashi Ayano Sato Kazuya Matsuda Tomohito Iwasaki Takuya Kanda Ryoko Uemura Hidetoshi Higuchi |
| author_facet | Koji Nishi Satoshi Gondaira Yuki Hirano Masahide Ohashi Ayano Sato Kazuya Matsuda Tomohito Iwasaki Takuya Kanda Ryoko Uemura Hidetoshi Higuchi |
| author_sort | Koji Nishi |
| collection | DOAJ |
| description | Abstract Mycoplasma pneumonia, caused by Mycoplasma bovis (Mycoplasmopsis bovis; M. bovis), is linked with severe inflammatory reactions in the lungs and can be challenging to treat with antibiotics. Biofilms play a significant role in bacterial persistence and contribute to the development of chronic lesions. A recent study has shown that polymicrobial interactions between species are an important factor in biofilm formation, yet the precise mechanism of biofilm formation in M. bovis remains unknown. By assuming multiple pathogen infections in the bovine respiratory disease complex (BRDC), this study examined the characterisation of the polymicrobial relationship between M. bovis and Trueperella pyogenes (T. pyogenes) during biofilm formation. Autopsies were performed on four Holstein calves (two chronic Mycoplasma pneumonia calves and two control calves). Bacterium-like aggregation structures (> 10 μm), which were assumed to be biofilms of M. bovis in vivo, were observed adhering to the cilia in calves with Mycoplasma pneumonia. M. bovis released an extracellular matrix to connect with neighbouring bacteria and form a mature biofilm on the plate. Biofilm formation in the co-culture of M. bovis and T. pyogenes (strain T1: 1 × 105 and 1 × 106 CFU/well) significantly increased (p < 0.05 and p < 0.01; 64.1% and 64.8% increase) compared to that in a single culture of these bacteria. Furthermore, some large aggregates (> 40 μm), composed of M. bovis and T. pyogenes, were observed. The morphological characteristics of this biofilm were similar to those observed in vivo compared to a single culture. In conclusion, the polymicrobial interaction between M. bovis and T. pyogenes induces biofilm formation, which is associated with increased resistance to antimicrobial agents, and this exacerbates the progression of chronic Mycoplasma pneumonia. |
| format | Article |
| id | doaj-art-8a655813cad44425992172f71ffe96bd |
| institution | Kabale University |
| issn | 1297-9716 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-8a655813cad44425992172f71ffe96bd2025-02-02T12:37:05ZengBMCVeterinary Research1297-97162025-01-0156111310.1186/s13567-025-01468-1Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenesKoji Nishi0Satoshi Gondaira1Yuki Hirano2Masahide Ohashi3Ayano Sato4Kazuya Matsuda5Tomohito Iwasaki6Takuya Kanda7Ryoko Uemura8Hidetoshi Higuchi9Animal Health Unit, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen UniversityAnimal Health Unit, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen UniversityAnimal Research Center, Agricultural Research Department, Hokkaido Research OrganizationAnimal Health Unit, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen UniversityLarge Animal Clinical Science, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen UniversityDepartment of Veterinary Pathology, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen UniversityDepartment of Food Science and Human Wellness, College of Agriculture, Food and Environment Science, Rakuno Gakuen UniversityFood Safety Laboratory, Faculty of Veterinary Medicine, Okayama University of ScienceAnimal Health, Faculty of Agriculture, University of MiyazakiAnimal Health Unit, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen UniversityAbstract Mycoplasma pneumonia, caused by Mycoplasma bovis (Mycoplasmopsis bovis; M. bovis), is linked with severe inflammatory reactions in the lungs and can be challenging to treat with antibiotics. Biofilms play a significant role in bacterial persistence and contribute to the development of chronic lesions. A recent study has shown that polymicrobial interactions between species are an important factor in biofilm formation, yet the precise mechanism of biofilm formation in M. bovis remains unknown. By assuming multiple pathogen infections in the bovine respiratory disease complex (BRDC), this study examined the characterisation of the polymicrobial relationship between M. bovis and Trueperella pyogenes (T. pyogenes) during biofilm formation. Autopsies were performed on four Holstein calves (two chronic Mycoplasma pneumonia calves and two control calves). Bacterium-like aggregation structures (> 10 μm), which were assumed to be biofilms of M. bovis in vivo, were observed adhering to the cilia in calves with Mycoplasma pneumonia. M. bovis released an extracellular matrix to connect with neighbouring bacteria and form a mature biofilm on the plate. Biofilm formation in the co-culture of M. bovis and T. pyogenes (strain T1: 1 × 105 and 1 × 106 CFU/well) significantly increased (p < 0.05 and p < 0.01; 64.1% and 64.8% increase) compared to that in a single culture of these bacteria. Furthermore, some large aggregates (> 40 μm), composed of M. bovis and T. pyogenes, were observed. The morphological characteristics of this biofilm were similar to those observed in vivo compared to a single culture. In conclusion, the polymicrobial interaction between M. bovis and T. pyogenes induces biofilm formation, which is associated with increased resistance to antimicrobial agents, and this exacerbates the progression of chronic Mycoplasma pneumonia.https://doi.org/10.1186/s13567-025-01468-1Antibioticsantimicrobial agentsbovine respiratory diseaseextracellular matrixtrachea |
| spellingShingle | Koji Nishi Satoshi Gondaira Yuki Hirano Masahide Ohashi Ayano Sato Kazuya Matsuda Tomohito Iwasaki Takuya Kanda Ryoko Uemura Hidetoshi Higuchi Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes Veterinary Research Antibiotics antimicrobial agents bovine respiratory disease extracellular matrix trachea |
| title | Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes |
| title_full | Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes |
| title_fullStr | Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes |
| title_full_unstemmed | Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes |
| title_short | Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes |
| title_sort | biofilm characterisation of mycoplasma bovis co cultured with trueperella pyogenes |
| topic | Antibiotics antimicrobial agents bovine respiratory disease extracellular matrix trachea |
| url | https://doi.org/10.1186/s13567-025-01468-1 |
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