Malignancy progression and treatment efficacy estimation of osteosarcoma patients based on in vitro cell culture model and analysis
Background: Osteosarcoma (OS) usually happens in patients under 20 years old and is notorious for its low survivorship and limb loss. Personalized medicine is a viable approach to increase the efficiency of chemotherapy which is the main prognostic factor for survivorship after surgical treatment. M...
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Elsevier
2025-07-01
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| Series: | Journal of the Formosan Medical Association |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664624003073 |
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| author | Hsi-Chieh Lin Kuei-Hsiang Hsu Jir-You Wang Wei-Ming Chen Yi-Chung Tung Yu-Ping Su |
| author_facet | Hsi-Chieh Lin Kuei-Hsiang Hsu Jir-You Wang Wei-Ming Chen Yi-Chung Tung Yu-Ping Su |
| author_sort | Hsi-Chieh Lin |
| collection | DOAJ |
| description | Background: Osteosarcoma (OS) usually happens in patients under 20 years old and is notorious for its low survivorship and limb loss. Personalized medicine is a viable approach to increase the efficiency of chemotherapy which is the main prognostic factor for survivorship after surgical treatment. Methods: In this five-year prospective observational study, we collected primary cells of osteosarcoma from 15 patients, and examined the correlation between clinical characters of patients and cell properties characterized using various in vitro assays. The properties including genes expression, pro-angiogenic capability and anti-cancer drug response are characterized respectively by using RT-PCR, tube formation assay, osteogenesis assay and drug testing on 3D tumor spheroid model. Result: The results suggest that OS patients with higher MMP9 expression levels have higher probability to develop skip metastasis (p = 0.041). The 3D tumor spheroid test based on the median lethal dose from 2D culture provides some prognostic value. Patients do not response well to methotrexate (MTX) show higher percentage of high pathology grade (p = 0.009) and lung metastasis (p = 0.044). Also, patients respond well to ifosfamide (IFO) have higher probability to achieve high tumor necrosis rate (p = 0.007). Conclusion: The association between cell properties and clinical characters of patients provided by our data can act as potential prognostic factors to help physicians to develop effective personalized chemotherapy for osteosarcoma treatments. |
| format | Article |
| id | doaj-art-8a5eaa79b1b84db3aef4eaf7d7e0be00 |
| institution | OA Journals |
| issn | 0929-6646 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj-art-8a5eaa79b1b84db3aef4eaf7d7e0be002025-08-20T02:20:37ZengElsevierJournal of the Formosan Medical Association0929-66462025-07-01124760761510.1016/j.jfma.2024.07.004Malignancy progression and treatment efficacy estimation of osteosarcoma patients based on in vitro cell culture model and analysisHsi-Chieh Lin0Kuei-Hsiang Hsu1Jir-You Wang2Wei-Ming Chen3Yi-Chung Tung4Yu-Ping Su5Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, TaiwanDepartment of Orthopedic and Traumatology, Taipei Veterans General Hospital, Taipei, 11217, Taiwan; Department of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, TaiwanDepartment of Orthopedic and Traumatology, Taipei Veterans General Hospital, Taipei, 11217, TaiwanDepartment of Orthopedic and Traumatology, Taipei Veterans General Hospital, Taipei, 11217, Taiwan; Department of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, TaiwanResearch Center for Applied Sciences, Academia Sinica, Taipei, 11529, TaiwanDepartment of Orthopedic and Traumatology, Taipei Veterans General Hospital, Taipei, 11217, Taiwan; Department of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan; Corresponding author. Department of Orthopedic and Traumatology, Taipei Veterans General Hospital, Taipei, 11217, Taiwan.Background: Osteosarcoma (OS) usually happens in patients under 20 years old and is notorious for its low survivorship and limb loss. Personalized medicine is a viable approach to increase the efficiency of chemotherapy which is the main prognostic factor for survivorship after surgical treatment. Methods: In this five-year prospective observational study, we collected primary cells of osteosarcoma from 15 patients, and examined the correlation between clinical characters of patients and cell properties characterized using various in vitro assays. The properties including genes expression, pro-angiogenic capability and anti-cancer drug response are characterized respectively by using RT-PCR, tube formation assay, osteogenesis assay and drug testing on 3D tumor spheroid model. Result: The results suggest that OS patients with higher MMP9 expression levels have higher probability to develop skip metastasis (p = 0.041). The 3D tumor spheroid test based on the median lethal dose from 2D culture provides some prognostic value. Patients do not response well to methotrexate (MTX) show higher percentage of high pathology grade (p = 0.009) and lung metastasis (p = 0.044). Also, patients respond well to ifosfamide (IFO) have higher probability to achieve high tumor necrosis rate (p = 0.007). Conclusion: The association between cell properties and clinical characters of patients provided by our data can act as potential prognostic factors to help physicians to develop effective personalized chemotherapy for osteosarcoma treatments.http://www.sciencedirect.com/science/article/pii/S0929664624003073OsteosarcomaChemotherapyAngiogenesisOsteogenesisSpheroid modelDrug efficacy |
| spellingShingle | Hsi-Chieh Lin Kuei-Hsiang Hsu Jir-You Wang Wei-Ming Chen Yi-Chung Tung Yu-Ping Su Malignancy progression and treatment efficacy estimation of osteosarcoma patients based on in vitro cell culture model and analysis Journal of the Formosan Medical Association Osteosarcoma Chemotherapy Angiogenesis Osteogenesis Spheroid model Drug efficacy |
| title | Malignancy progression and treatment efficacy estimation of osteosarcoma patients based on in vitro cell culture model and analysis |
| title_full | Malignancy progression and treatment efficacy estimation of osteosarcoma patients based on in vitro cell culture model and analysis |
| title_fullStr | Malignancy progression and treatment efficacy estimation of osteosarcoma patients based on in vitro cell culture model and analysis |
| title_full_unstemmed | Malignancy progression and treatment efficacy estimation of osteosarcoma patients based on in vitro cell culture model and analysis |
| title_short | Malignancy progression and treatment efficacy estimation of osteosarcoma patients based on in vitro cell culture model and analysis |
| title_sort | malignancy progression and treatment efficacy estimation of osteosarcoma patients based on in vitro cell culture model and analysis |
| topic | Osteosarcoma Chemotherapy Angiogenesis Osteogenesis Spheroid model Drug efficacy |
| url | http://www.sciencedirect.com/science/article/pii/S0929664624003073 |
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